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Channel-Transporter Correlation

Research Project

Project/Area Number 07276103
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionOkazaki National Research Institutes

Principal Investigator

OKADA Yasunobu  National Institute for Physiological Sciences, Professor, 生理学研究所, 教授 (10025661)

Co-Investigator(Kenkyū-buntansha) MARUMO Fumiaki  Tokyo Med. Dent. Univ., Professor, 医学部, 教授 (00050443)
TSUCHIYA Tomofusa  Okayama University, Professor, 薬学部, 教授 (80012673)
UEDA Kazumitsu  Kyoto University, Research Associate, 農学部, 助手 (10151789)
IMAI Masashi  Jichi Medical University, Professor, 教授 (40049010)
TAKEGUCHI Noriaki  Toyama Med. Pharmac. Univ., Professor, 薬学部, 教授 (00019126)
Project Period (FY) 1995 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥87,800,000 (Direct Cost: ¥87,800,000)
Fiscal Year 1998: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1997: ¥26,800,000 (Direct Cost: ¥26,800,000)
Fiscal Year 1996: ¥28,500,000 (Direct Cost: ¥28,500,000)
Fiscal Year 1995: ¥29,500,000 (Direct Cost: ¥29,500,000)
Keywordsion channel / water channel / transporter / Ca pump / structure-function relation / cloning
Research Abstract

Structural and functional correlations of channels and/or transporters were investigated from physiological, molecular biological and biochemical points of view. During these three years in this study, new channels (over 29) and new transporters (over 42) were discovered, and their genes were cloned. Structure-function correlations were elucidated by identification of numbers of functional domains and amino acid sites. New types of functional or structural correlation between channels and transporters were also demonstrated (over 10). New diseases were identified to be due to mutations of genes of channels or transporters (over 10). Especially a big progress was achieved in the research of ATP-binding cassette (ABC) transporter proteins. For example, a novel ABC protein cMOAT was cloned and found to mediate the ATP-dependent transport of glutathion-conjugates. The expression of cMOAT was found to be defective in the Dubin-Johnson syndrome. The ATP-sensitive KィイD1+ィエD1 channel was revealed to be a complex of two subunits : SUR1 of the ABC protein family and Kir6.2 of the inward-rectifier KィイD1+ィエD1 channel family. Some of ABC proteins have been found to have more than one activities of transporter, channel and their regulator. For example, P-glycoprotein and CFTR were found to be a regulator for volume-sensitive ClィイD1-ィエD1 channel and ATP release, respectively.

Report

(5 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (47 results)

All Other

All Publications (47 results)

  • [Publications] Tsumura,Hazama & Okada: "Activation of cAMP-dependent CI corrents in guinea-pig Paneth cells without relevant evidence for CFTR expression."Journal of Physiology. 512・3. 765-777 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Zhou,Hazama & Okada: "Tyrosine kinase-independent extracellular action of genistein on the CFTR CI channel in guinea pig ventricular myocytes and CFTR-transfected mouse fibroblasts."Japanese Journal of Physiology. 48・5. 389-396 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sabirov,Morishima & Okada: "Probing the water permeability of ROMK1 and amphotericin B channels using Xenopus oocytes."Biochimica Biophysica Acta. 1368. 19-26 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 岡田泰伸: "CIチャネルと細胞容積調節および細胞死"Mebio. 15・6. 61-68 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 森島 繁、岡田泰伸: "イオンチャネルの構造と機能"Mebio. 15・6. 12-24 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 岡田泰伸、小島 至: "チャネルとトランスポータの構造と疾患"Molecular Medicine. 35. 4-17 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Okada: "Cell Volume Regulation:The Molecular Mechanism and Volume Sensing Machinery"Elsevier,Amsterdam. 214 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Okada: "Cell Volume Regulation(Ed.F.Lang)"Karger,Basel. 12

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] R. Z. Sabirov, S. Morishima & Y. Okada: "Probing the water permeability of ROMK1 and amphotericin B channels using Xenopus oocytes"Biochem. Biophys. Acta. 1368. 19-26 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T. Nakamura, S. Arii, K. Monden, M. Furutani, Y. Takeda, M. Imamura, M. Tominaga & Y. Okada: "Expression of the Na/Ca exchanger emerges in hepatic stellate cells after activation in association with liver fibrosis"Proc. Natl. Acad. Sci. USA. 95. 5389-5394 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y. Liu, S. Oiki, T. Tsumura, T. Shimizu & Y. Okada: "Glibenclamide blocks volume-sensitive ClィイD1-ィエD1 channels by dual mechanisms"Am. J. Physiol.. 275. C343-C351 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y. Okada, S. Oiki, A. Hazama & S. Morishima: "Criteria for the molecular identification of the volume-sensitive outwardly rectifying ClィイD1-ィエD1 channel"J. Gen. Physiol.. 112. 1-3 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T. Higuchi, S. Suga, T. Tsuchiya, H. Hisada, S. Morishima, Y. Okada & M. Maeshima: "Molecular cloning, water channel activity and tissue specific expression of two isomers of radish vacuolar aquaporin"Plant Cell Physiol.. 39. 905-913 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T. Tsumura, A. Hazama, T. Miyoshi, S Ueda & Y. Okada: "Activation of cAMP-dependent ClィイD1-ィエD1 currents in guinea-pig Paneth cells without relevant evidence for CFTR expression"J. Physiol. (London). 5212. 765-777 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A. Hazama, S. Hayashi & Y. Okada: "Cell surface measurements of ATP release from single pancreatic β cells using a novel biosensor technique"Pflugers Arch. Eur. J. Physiol.. 437. 31-35 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S. -S. Zhou, A. Hazama & Y. Okada: "Tyrosine kinase-independent extracellular action of genistein on the CFTR ClィイD1-ィエD1 channel in guinea pig ventricular myocytes and CFTR-transfected mouse fibroblasts"Jpn. J. Physiol.. 48. 389-396 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y. Okada: "Cell Volume regulation : The molecular Mechanism and Volume Sensing Machinery"Elsevier, Amsterdam. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y. Okada: "Cell volume-sensitive chloride channel. In, "Cell Volume Regulation" (ed. F. Lang)"Karger, Basel. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Okada et al.: "Criteria for the molecular identification of the volume-sensitive outwardly rectifying Cl^- channel." Journal of General Physiology. 112. 1-3 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tsumura,Hazama & Okada: "Activation of cAMP-dependent Cl^- currents in guinea-pig Paneth cells without relevant evidence for CFTR expression." Journal of Physiology. 512・3. 765-777 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 森島 繁・岡田泰伸: "イオンチャネルの構造と機能" Mebio. 15・6. 12-24 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 岡田泰伸: "Clチャネルと細胞容積調節および細胞死" Mebio. 15・6. 61-68 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 岡田泰伸: "Cl^-チャネルの機能構造と疾患" 神経研究の進歩. 42. 263-278 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 岡田泰伸・小島 至: "チャネルとトランスポータの構造と疾患" Molecular Medicine. 35. 4-17 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Okada: "Cell Volume Regulation:The Molecular Machanism and Volume Sensing Machinery" Elsevier, 1-214 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 岡田泰伸: "Volume expansion-sensing outward-rectifier Cl^- channel : fresh start to the molecular identity and volume sensor." Am.J.Physiol.273. C755-C789 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 植田和光, ら: "MgADP antagonism to the Mg-independent ATP binding of the sulfonylurea receptor SUR1." J.Biol.Chem.272. 22983-22986 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 土屋友房, ら: "Inhibition of thymine transport in dnaA mutants of Escherichia coli." J.Biol.Chem.272. 21195-21200 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 竹口紀晃, ら: "Mutational analysis of putative SCH 28080 binding sites of the gastric H^+,K^+-ATPase." J.Biol.Chem.272. 17668-17674 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 丸茂文昭, ら: "Phosphorylation of serine 256 is required for cAMP-dependent regulatory exocytosis of the aquaporin-2 water channel." J.Biol.Chem.272. 14800-14804 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 老木成稔、岡田泰伸, ら: "A conserved arginine residue in the pore region of an inward rectifier K channel (IRK1) as an external barrier for cationic blockers." J.Gen.Physiol.110. 665-677 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 岡田泰伸・清野 進: "チャネルとトランスポータ:その働きと病気" メジカルビュー社, 190 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 岡田泰伸ら: "Sensitivity of volume-sensitive Cl^- conductance in human epithelial cells to extracellular nucleotides." Am.J.Physiol.271. 27887-27893 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 植田和光ら: "Aureoblasidin A,an antifungal cyclic depsipeptide antibiotic,is a substrate for both human MDR1 and MDR2/P-glycoprotein." FEBS Lett.399. 29-32 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 土屋友房ら: "Cloning and sequencing of the gene for Na^+/H^+ antiporter of Vibrio parahaemolyticus." Biochem.Biophys.Res.Commun.222. 774-779 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 竹口紀晃ら: "Functional expression of gastric H^+,K^+-ATPase and site-directed mutagenesis of the putative cation binding site and the catalytic center." J.Biol.Chem.271. 2740-2745 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 丸茂文昭ら: "Identification of a new outwardly rectifying Cl channel that belongs to a subfamily of the ClC Cl channels." J.Biol.Chem.271. 10210-10216 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 老木成稔、岡田泰伸ら: "Two-sided action of protons on an inward rectifier K^+ channel (IRK1)." Pflugers Arch.433. 428-434 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 岡田泰伸: "最新内科学大系2.科学としての内科学 チャネルとトランスポータとその異常" 中山書店(井村裕夫ら編), 372(内12ページ分担) (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 岡田泰伸: "パッチクランプ実験技術法" 吉岡書店, 185 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 岡田泰伸ら: "チャネルとトランスポータ" 最新医学. 51. 5-7 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 植田和光ら: "Cepharanthin,a multidrug resistant modifier,is a substrate for P-glycoprotein" J.Pharmacol.Exp.Ther.275. 73-78 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 土屋友房ら: "Essential aspartic acid residues,Asp-133,Asp-163 and Asp-164,in the transmembrane helices of a Na/H antiporter" FEBS Lett.363. 264-268 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 丸茂文昭ら: "Localization and functional characterization of rat kidney-specific chloride channel,C1C-K1" J.Clin.Inv.95. 104-113 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 竹口紀晃ら: "A gastric housekeeping C1^- channel activated via prostaglandin EP3.receptor-urediated pathway." J.Biol.Chem.270. 18781-18785 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 今井 正ら: "Immunohistochemical localization of pH-sensitive K^+ channel,RACTK1" An.J.Physiol.269. C496-C503 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 富永真琴・岡田泰伸: "分子医科学シリーズ2 細胞:構造と機能" メディカル・ビュー社(月田承一郎編), 202(22) (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2019-02-15  

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