• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular mechanism of synaptic plasticity

Research Project

Project/Area Number 07278103
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionThe University of Tokyo

Principal Investigator

SHIMIZU Takao  UNIVERSITY OF TOKYO, GRADUATh SCHOOL OF MIEDICINE, PROFESSOR, 大学院・医学系研究科, 教授 (80127092)

Co-Investigator(Kenkyū-buntansha) SHIBUKI Katsuei  NIIGATA UNIVERSITY, INSTITUTE OF BRAIN RESEARCH, PROFESSOR, 脳研究所, 教授 (40146163)
KAIBUCHI Kozo  Nara INSTLTUTE OF SCIENCE and TECHNOLOGY,DEPARTMENT OF CELL-BIOLOGY, PROFESSOR, バイオサイエンス研究科, 教授 (00169377)
KASAI Haruo  Okazaki, NATIONAL INSTITUTh OF PHYSIOLOGICAL SCIENCES, PROFESSOR, 生理学研究所, 教授 (60224375)
KUDO Ichoro  SHOWA UNIVERSITY, FACULTY OF PHARMACEUTICAL SCIENCES, PROFESSOR, 薬学部, 教授 (30134612)
MIYAZAKI Jyunichi  OSAKA UNIVERSITY, SCHOOL OF MIEDICINE, PROFESSOR, 大学院・医学研究科, 教授 (10200156)
後藤 由希子  京都大学, ウイルス研究所, 助手 (70252525)
成宮 周  京都大学, 医学部, 教授 (70144350)
Project Period (FY) 1995 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥224,100,000 (Direct Cost: ¥224,100,000)
Fiscal Year 1998: ¥47,300,000 (Direct Cost: ¥47,300,000)
Fiscal Year 1997: ¥51,000,000 (Direct Cost: ¥51,000,000)
Fiscal Year 1996: ¥61,800,000 (Direct Cost: ¥61,800,000)
Fiscal Year 1995: ¥64,000,000 (Direct Cost: ¥64,000,000)
Keywordslipid mediators / protein kinase C / phospholipases / 血小板活性化因子 / プロスタグランジン / ノックアウトマウス / 低分子量Gタンパク / 無脳回症 / シグナル伝達 / 開口放出 / ホスホリパーゼA_2 / Miller-Dieker / グリア細胞 / プロスタノイド / 神経可塑性 / 低分子量Gタンパク質
Research Abstract

Various enzymes and receptors for lipid niediators (prostaglandins, leukotrines, platelet-activating factor etc.) have been isolated and their knockout mice were established by the members of this project. By analyzing their phenotypes, it was found that these mediators arc involved in brain functions, synaptogeneses, and neuronal cell development, working in concert with glutamate and other neurotransmitters. we also identified regulations of various types of kinases (protein kinases C, calcium calmodulin-dependent kinases, MAP kinases etc) during synaptic transmission and plasticity. Several small sized GTP binding proteins, and related molecules (SNARE, CREB) are identified of their roles in the synaptic plasticity.

Report

(5 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Kimura, K. et al.: "Regulation of myosin phosphorylation by Rho and Rho-associated kinase(Rho-Kinase)"Science. 273. 245-248 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ho, YS et al.: "Brain acetylhydrolase that inactivates platelet-activating factor (PAF) is a G-protein-like trimer"Nature. 385. 89-93 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yokomizo, T. et al.: "A G-protein-coupled receptor for leukotriene B4 that mediates chemotaxis"Nature. 387. 620-624 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ushikubi, F. et al.: "Impaired febrile response in mice lacking the prostaglandin E receptor subtype EP^3"Nature. 395. 281-284 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Okuda-Ashitaka, E.: "Nocistatin : a peptide blocking nociceptin action in pain transmission"Nature. 392. 286-289 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kasai, H. et al.: "Multiple and diverse forms of regulates exocytosis revealed in wild-type and defective PC12 cells"Proc. Natl. Acad. Sci. U.S.A.. 96. 945-949 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kimura, K. et al.: "Regulation of myosin phosphorylation by Rho and Rho-associated kinase (Rho-kinase)"Science. 273. 245-248 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ho, YS et al.: "Brain acetyihydrolase that inactivates platelet-activating factor (PAF) is a G-protein-like trimer"Nature. 385. 89-93 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yokomizo, T. et al.: "A G-protein-coupled receptor for leukotriene B4 that mediates chemotaxis"Nature. 387. 620-624 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ushikubi, F. et al.: "Impaired febrile response in mice lacking the prostaglandin E receptor subypte EP3"Nature. 395. 281-284 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Okuda-Ashitaka, E. et al.: "Nocistatin : a peptide blocking nociceptin action in pain transmissioin"Nature. 392. 286-289 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kasai, H. et al.: "Multiple and diverse forms of regulates exocytosis revealed in wild-type and defective PC12 cells"Proc. Nati. Acad. Sci. U.S.A. 96. 945-949 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ishii,S et al: "Impaired anaphylactic responses with intact sensitivity to endotoxin in mice lacking a PAF reccptor" J.Exp.Med. 187. 1779-1788 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kasai,H.: "Comparative biology of Ca-dependent exocytosis:implications of Kinetic diversity for secretory function" Trends Neurosci,. 22. 88-93 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kuroda,S・et al.: "Role of IQGAP1,a target of the small GTPAues cdc42 and rac1,inregulation of F-cardherio mediated cell-allrahesim" Science. 281. 832-835 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kimura,S et al: "cAMP-dependent long-term potentiation of nitric oxide release from cerebellar parellelfibers in rats" J.Neurosci. 18. 8551-8558 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Miki,T.et al.: "Defective insulin scretion and enhanced insulin action in KATP Channel deficient mice" Proc.Natl.Acad.Sci.USA. 95. 10402-10406 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Manyu,H.et al: "Switching of platelet-activating factor acetylhydrolase catalytic subunits developing rat brain" J.Biol.Chem.273. 18567-18572 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ho,YS et al.: "Brain acetylhydrolase that inactivates plaeelet-activating factor is a G-protein-like trimer" Nature. 385. 89-93 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Murata,T et al.: "Altered pain perception and inflammatory response in mice lacking prostacyclin receptor." Nature. 388. 678-682 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sone,M.et al.: "Still-life a protein homologous to GDP-GTP exchangeser in synaptic leminal." Science. 275. 543-547 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ninomiya,A.et al.: "Kinetic diversity in the fusion of exocytotic vesicles." EMBO Journal. 16. 929-934 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yokomizo,T.et al.: "A G-protein-coupled receptor for leukotriene B4 that mediates chemotaxis." Nature. 390. 620-624 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shigemoto,R.et al.: "Metabotropic glutamate receptor subtypes in the rat hippocampus." J.Neuroscience.17. 7503-7522 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mori,M.,et al.: "Predominant expression of platelet-activating factor receptor in the rat brain microglia." J.Neurosci.16. 3590-3600 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kasai,H.,at al.: "Two components of exocytosis and endocytos is in phaeochromocytoma cells sutudied using caged-Ca2+compounds." J.Physiology. 494. 53-65 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kimura,K.,et al.: "Regulation of myosin phosphatase by Rho and Rho-associated kinase(Rho-kinase)." Science. 273. 245-248 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shibuki,K.,et al.: "Dynamic properties of nitric oxide release from parallel fibres in rat cerebellar slices." J.Physiology. 498. 443-452 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sunaga,S.,et al.: "Efficient reomval of loxP-flanked sequences in a gene targeted locus by transient expression of Cre recombinase in fertilized eggs." Mol. Reprod.& Dev.46. 109-113 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Murakami,M.,et al.: "Type II Secretory Phospholipase A2 Associated with Cell Surfaces via C-terminal Heparin-binding Lysine Residues Augments Stimulus-initiated Delayed Prostaglandin Generation." JBC. 271・47. 30041-30051 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Watanabe, G. et al.: "Proteinkinase N(PKN) and PKN-related protein rhophilin as targets of small GT Pase rho" Sciene. 271. 645-648 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Amano, M. et al.: "Identification of a putative target for rho as the serinethreonine kinase protein kinase N" Sciene. 271. 648-650 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kashiwabuchi, N. et al.: "Impairement of motor coordination, Purkinje cell synapse formation and cerebellar LTD in Glu R δ2 mutant mice" Cell. 81. 245-252 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fukuda, M. et al.: "Induction of neurite outgrowth by MAP kinase in PC12 cells" Oncogene. 11. 239-244 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Araki, K. et al.: "Site-specific recombination of a tramsgene in fertilized rggs by transient expression of Cre recombinase" Proc. Natl. Acad. Sci. USA. 92. 160-164 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Mutoh, H. et al.: "Tissue-specitic response of the human platelet-activating factor receptor gene to refinaic acid and thyroid hormone" Proc. Natl. Acad. Sci. USA. 93. 774-779 (1996)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2019-02-15  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi