GENETIC DISSECTION OF SEXUAL DIFFERENTIATION AND POLLINATION

• Project/Area Number: 07281102
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas (A)
• Allocation Type: Single-year Grants
• Research Institution: Tohoku University
• Principal Investigator: HINATA Kokichi 東北大学, 農学部, 名誉教授 (00005589)
• Co-Investigator(Kenkyū-buntansha): TORIYAMA Kinya 東北大学, 大学院・農学研究科, 助教授 (20183882) ; KOMEDA Yoshibumi 北海道大学, 大学院・理学研究科, 教授 (10124215) ; ISOGAI Akira 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (20011992) ; NAGATO Yasuo 東京大学, 大学院・農学生命科学, 教授 (10143413) ; KAMADA Hiroshi 筑波大学, 生物科学, 教授 (00169608)
• Project Period (FY): 1999
• Project Status: Completed (Fiscal Year 1999)
• Budget Amount: ¥85,300,000 (Direct Cost: ¥85,300,000); Fiscal Year 1999: ¥3,000,000 (Direct Cost: ¥3,000,000); Fiscal Year 1998: ¥20,000,000 (Direct Cost: ¥20,000,000); Fiscal Year 1997: ¥20,700,000 (Direct Cost: ¥20,700,000); Fiscal Year 1996: ¥19,500,000 (Direct Cost: ¥19,500,000); Fiscal Year 1995: ¥22,100,000 (Direct Cost: ¥22,100,000)
• Keywords: Higher plants / Sexual differentiation / Pollination / Fertilization / Pollen / Flower / Self-incompatibility / Embryo

Research Abstract

高等植物の多くは「両性花」をつけ、その中に雌性器官と雄性器官を分化させ、それらから形成された雌・雄配偶体が相互に情報を交換しながら受精し、胚発生につながる。これら雌・雄器官および配偶体の分化発達のスイッチ機構、受粉の際の自己・非自己の細胞間認識機構を分子生物学的手法を用いて解明し、有性生殖過程の基礎知見を得ることは、生命を理解する上で重要な研究領域である。本研究(平成7年度〜平成10年度)では、雌雄両性の分化・成熟過程と、受粉・受精過程の2つに焦点を絞り、それに関連する遺伝子を解剖吟味し、植物の生殖機構の解明を目指してきた。
平成11年度の本研究では、これまでの成果をとりまとめ、発表論文リスト、および、精選論文集を以下のように作製した。
(1)研究の概要(和文) 7ページ
(2)発表論文リスト 合計 528報
(3)精選論文別刷り集(ひとり5報以内)合計 172報収録
以上を2分冊(合計 1159ページ)として作製し、関係者に配布した。
また、研究成果を一般学術図書として出版するため、平成12年度研究成果公開申請費を申請中である。

Research Products

• [Publications] Takasaki T., Hatakeyama K., Suzuki G., Watanabe M., Isogai A., Hinata K.: "The S receptor kinase determines self-incompatibility in Brassica stigma"Nature. 403. 913-916 (2000)
• [Publications] Takayama S., Shiba H., Iwano M., Shimosato H., Che F-S., Kai N., Watanabe M., Suzuki G., Hinata K. and Isogai A.: "The pollen determinant of self-incompatibility in Brassica campestris"Proc. Nat. Acad. Sci., USA. 97. 1920-1925 (2000)
• [Publications] Takayama S., Shiba H., Iwano M., Asano K., Hara M., Che F-S., Watanabe M., Hinata K. and Isogai A.: "Isolation and characterization of pollen coat protein of Brassica campestris that interact with S-locus-related glycoprotein I involved in pollenstigma adhesion"Proc. Nat. Acad. Sci., USA. (in press). (2000)
• [Publications] Suzuki G, Kai N, Hirose T, Fukui K, Nishio T, Takayama S, Isogai A, Watanabe M, Hinata K.: "Genomic organization of the S locus. Identification and characterization of genes in SLG/SRK region of S9 haplotype of Brassica campestris (syn. rapa)"Geneties. 153. 391-400 (1999)
• [Publications] Takasaki T., Hatakeyama K., Watanabe M., Toriyama K., Isogai A. and Hinata K.: "Induction of SLG (S-locus glycoprotein) alters the phenotype of endogenous S-haplotype, but confers no new S-haplotype specificity in Brassica rapa L"Plant Mol. Biol.. 40. 659-668 (1999)
• [Publications] Kato A., M.Suzuki, A.Kuwah ara, H.Ooe, K.Higano-Inaba and Y.Komeda: "Isolation and analysis of cDNA within a 300 kb Arabidopsis thaliana genomic region located around the 100 map unit of chromosome 1. e 239:309-316"Geneties. 239. 309-316 (1999)
• [Publications] Akamatsu T., Y.Hanzawa, Y.Ohtake, T.Takahashi, K.Nishitani and Y.Komeda: "Expression of endoxyloglucan transferase genes in acaulis mutants of Arbidopsis"Plant Physiology. 121. 715-722 (1999)
• [Publications] Okada T., Z.Zhang, S.D.Russell and K.Toriyama: "Localization of the Ca2+-binding protein, Bra r 1, in anthers and pollen tubes"Plant Cell Physiol.. 40. 1243-1252 (1999)
• [Publications] Satoh N., S-K Hong, A.Nishimura, A.Matsuoka, H.Kitano and Y.Nagato: "Initiation of shoot apical meristem in rice : characterization of four SHOOTLESS genes"Development. 126. 3629-3636 (1999)
• [Publications] Kobayashi T., C.Eun, H.Hanai, Y.Matsubayashi, Y.Sakagami and H.Kameda: "Phytosulfokine-a, a peptidyl plant growth factor, stimulates cell divition that leads to somatic embryogenesis in carrot"J. Exp. Bot.. 50. 1123-1128 (1999)
• [Publications] Kobayasi T., K.Higashi, T.Saitou and H.Kameda: "Physiological properties of inhibitory conditioning factor(s) in high cell density cultures of carrot cells during somatic embryogenesis"Plant Sci.. 144. 69-75 (1999)
• [Publications] Hatakeyama, K., Watanabe, M., Takasaki, T., Ojima, K. and Hinata, K.: "Dominance relationships between S-alleles in self-incompatible Brassica campestris L."Heredity. 79. 241-247 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hatakeyama, K., Takasaki, T., Watanabe, M. and Hinata, K.: "Molecular characterization of S locus genes, SLG and SRK, in a pollen-recessive self-incompatibility haplotype of Brassica rapa L."Genetics. 194. 1587-1597 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yokoyama, R., Takahashi, T., Kato, A., Torii K.U. and Komeda Y.: "The Arabidopsis ERECTA gene is expressed in the shoot apical meristem and organ primordia"The Plant Journal. 15. 301-310 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Toriyama, K., Hanaoka, K., Okada, T., Watanabe, M.: "Molecular cloning of a cDNA encoding a pollen extracellular protein as a potential source of a pollen allergen in Brassica rapa"FEBS Let. 424. 234-238 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tachikawa,Y., Saitou,T., Kamada H. and Harada, H.: "Changes in protein pattern during stress-induction of carrot (Daucus carota L.) somatic embryogenesis"Plant Bioptechnology. 15. 17-22 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Takahashi, M., Nagasawa, N., Kitano, H. and Nagato, Y.: "Panicle phytomer 1 mutations affect the panicle architecture of rice"Theoret. Appl. Genet.. 96. 1050-1056 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Akashi, K., Takenaka, M., Yamaoka, S., Suyama, Y., Fukuzawa, H. and Ohyama, K.: "Coexisitence of nuclear DNA-encoded tRNAVal(AAC) and mitochondrial DNA-encoded tRNAVal(UAC) in mitochondria of liverwort Marchantia polymorpha"Nucl. Acids Res.. 26. 2168-2172 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Iwano, M., Sakamoto, K., Suzuki G., Watanabe, M., Takayama, S., Fukui, K., Hianata, K. and Isogai, A.: "Visualization of a self-incompatibility gene in Brassica campestris L. multicolor FISH"Theor. Appl. Genet. 751-757. 96. 751-757 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Natakeyama K, Watanabe M, Takasaki T, Ojima K, Hinata K: "Dominance relationship between S-alleles in self-incompatible Brassica campestris L"Heridity. 79. 241-247 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Harakeyama K, Takasaki T, Watanabe M, Hinata K: "Molecular characterzation of S locus genes, LG and SRK, in a pollen-recessive self-incompatibility haplotype of Brassica rapa L"Genetics. 194. 1587-1597 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yakoyama R, Takasaki T, ato A, Toni K, Komeda Y: "The Arabidopsis ERECTA gene is expressed in the shoot apical meristem and organ primordia"Plant J. 15. 301-310 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Toriyama K, Hanaoka K, Okada T, Watanabe M: "Molecular cloning of a c DNA encoding a pollen extracelluar protein as a potential source of a pollen allergen in Brassica rapa"FEBS Let. 424. 234-238 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Tachikawa Y, Saitou T, Kamada H, Harada: "Changes in protein pattern during stress-induction of carrot (Daucus carota L.) somatic embryogenesis"Plant Biol. 15. 1050-1056 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Takahashi M, Nagasawa N, Kitano H, Nagato: "Panicle phytomer 1 mutations affect the panicle architecture of rice"Theoret Appl Genet. 96. 1050-1056 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Akashi K, Takenaka M, Yamaoka S, Suyama Y, Fukuzawa H, Ohyama K: "Coexistence of nuclear DNA-encoded tRNAVal (ACC) and mitochndrial DNA-encoded tRNAVal (UAC) uin mirochndria of liverwort Marchantia polymorpha"Nucl. Aciods Res. 26. 2168-2172 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Iwano M, Sakamoto K, Suzuki C, Watanabe M, Takayama S. Fukui K, Hinata K, Isogai A: "FVisualization of a self-incompatibility gene in Brassica campestris L. by multicolor FISH"Theoret Appl Genet. 96. 751-757 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hatakeyama,K.,Watanabe,M.,Takasaki,T.,Ojima,K.and Hinata,K.: "Dominance relationships between S-alleles in self-incompatible Brassica campestris L." Heredity. 79. 241-247 (1998)
• [Publications] Hatakeyama,K.,Takasaki,T.,Watanabe,M.and Hinata,K.: "Molecular characterization of S locus genes,SLG and SRK,in a pollen-recessive self-incompatibility haplotype of Brassica rapa L." Genetics. 194. 1587-1597 (1998)
• [Publications] Yokoyama,R.,Takahashi,T.,Kato,A.,Torii K.U.and Komeda Y.: "The Arabidopsis ERECTA gene is expressed in the shoot apical meristem and organ primordia." The Plant Journal. (1998)
• [Publications] Toriyama,K.,Hanaoka,K.,Okada,T.,Watanabe,M.: "Molecular cloning of a cDNA encoding a pollen extracellular protein as a potential source of a pollen allergen in Brassica rapa." FEBS Let. 424. 234-238 (1998)
• [Publications] Tachikawa,Y.,Saitou,T.,Kamada H.and Harada,H.: "Changes in protein pattern during stress-induction of carrot (Daucus carota L.)somatic embryogenesis." Plant Bioptechnology. 15. 17-22 (1998)
• [Publications] Takahashi,M.,Nagasawa,N.,Kitano,H.and Nagato,Y.: "Panicle phytomer 1 mutations affect the panicle architecture of rice." Theoret.Appl.Genet.96. (1998)
• [Publications] Akashi,K.,Takenaka,M.,Yamaoka,S.,Suyama,Y.,Fukuzawa,H.,and Ohyama,K.: "Coexistence of nuclear DNA-encoded tRNAVal(AAC) and mitochondrial DNA-encoded tRNAVal(UAC) in mitochondria of liverwort Marchantia polymorpha." Nucl.Acids Res.,. 26. 2168-2172 (1998)
• [Publications] Iwano,M.,Sakamoto,K.,Suzuk G.,Watanabe,M.,Takayama,S.,Fukui,K.,Hinata,K.and Isogai,A.: "Visualization of a self-incompatibi lity gene in Brassica camestris L.by multicolor FISH." Theor.Appl.Genet.96. 751-757 (1998)
• [Publications] Suzuki,G., Watanabe,M., Isogai,A. and Hinata,K.: "Highly conserved 5'-flanking regions of two self-incompatibility genes,SLG9 and SRK9." Gene. 191. 123-126 (1997)
• [Publications] Takasaki,T., Hatakeyama,K., Ojima,K., Watanabe,M., Toriyama,K. and Hinata,K.: "Factors influencing Agrobacterium-mediated transformation of Brassica rapa L." Breed.Sci.47. 127-134 (1997)
• [Publications] Suzuki,G., Watanabe,M., Toriyama,K., Isogai,A. and Hinata,K.: "Direct cloning of the Brassica S locus by using a Pl-derived artificialchromosome (PAC) vector." Gene. 199. 133-137 (1997)
• [Publications] Suzuki,G., Watanabe,M., Kai,N., Matsuda,N., Toriyama,K., Takayama,S., Isogai,A. and Hinata,K.: "Three members of the S-multigene family are linked to the S locus of Bressica." Mol.Gen.Genet.256. 257-264 (1997)
• [Publications] Watana,M., Ono,T., Hatakeyama,K., Takayama,S., Isogai,A. and Hinata,K.: "Molecular characterization of SLG and S-related genes in a self-compatible Brassica campersris L.var.yellow sarson." Sex.Plant Reprod.10. 332-340 (1997)
• [Publications] Hanzawa,Y., Takahashi,T. and Komeda,Y.: "ACL5:an Arabidopsis gene required for the internodal elongation after flowering." The Plant Journal. 12(4). 863-874 (1997)
• [Publications] Yokoi,S, Tsuchiya T, Toriyama K, Hinata K: "Tapetum-specific expression of the Osg6B promoter-beta-glucuronidase gene in transgenic rice." Plant Cell Rep.16. 363-367 (1997)
• [Publications] Matuda N, Kishitani S, Toriyama K: "Antisense and sense CHS cDNA expressed by a tapetum-specific promoter causes partial male sterility in transgenic tobacco." Plant Biotechnology. 14. 157-161 (1997)
• [Publications] Abe F, Kitashiba H, Kishitani S, Toriyama K: "Isolation of a cDNA clone encoding the altemative oxidase expressed in rece anthers." Sex Plant Reprod. 10. 374-375 (1997)
• [Publications] Kitashiba H and Toriyama K: "Expression of a gene for a protein similar to HIV-1 Tat binding protien 1 (TBP1) in floral prgans of Brassica rapa." Plant Cell Physiol. 38. 966-969 (1997)
• [Publications] A.Ojima, H.Shiota, K.Higashi, H.Kmada, Y.Shinma, M.Wada and S.Satoh: "An extracellular insoluble inhibitor of cystein proteinases in cellcultures and seeds of carrot." Plant Mol.Biol.,. 34. 99-109 (1997)
• [Publications] K.Osuga, H.Kamada and A.Komamine: "Frequency improvement of somatic embryogensis at high embryo denstiy by partial replacement of medium in carrot suspension cultures." J.Fermentation and Bioengineering. 84. 275-278 (1997)
• [Publications] K.Sage-Ono, M.Ono, H.Harada and H.Kamada: "Dark inducible accumulation of mRAN for a translationally controlled trmor protein (TCTP) homolog in Pharbitis nil." Plant Cell Physiol.,. (accepted). (1998)
• [Publications] K.Sage-Ono, M.Ono, H.Harada and H.Kamada: "Accumulation of a clockregulated transcript during flower-inductive darkness in Pharbitis." Plant Physiol.,. (accepted). (1998)
• [Publications] Takahashi,M., Nagasawa,N., Kitano,H. and Nagato,Y.: "Panicle phytomes 1 mutations affect panicle architecture of rice." Theoret.Appl.Genet.(in press). (1998)
• [Publications] A.Estiati, T.Kubo, T.Mikami: "The ribosomal protein S7 gene is transcribed and edited in sugar beet mitochondria." Physiologia Plantaurum. (in press). (1998)
• [Publications] M.Senda, Y.Onodera, T.Mikami: "Recombination events across the atpA-associated repeated sequences in the mitochondrial genomes of beets." Theoretical and Applied Genetics. (in press). (1998)
• [Publications] M.Senda, Y.Onodera, T.Mikami: "Cytoplasmic diversity in leaf beet cultivars as revealed by mitochondrial DNA analysis." Hereditas. (in press). (1998)
• [Publications] Nishio, T., Kusaba, M.,Watanabe, M. and Hinata, K: "Registration of S alleles in Brassica campestris L. by the restriction fragments of SLGs" Theor. Appl. Genet. 92. 388-394 (1996)
• [Publications] Suzuki, G., Watanabe, M., Toriyama, K., Isogai, A., Hinata, K.: "Expression of SLG9 and SRK9 genes in transgenic tobacco." Plant Cell Physiol. 37. 866-869 (1996)
• [Publications] Matsushita, M., Watanabe, M., et al, Isogai, A., and Hinata, K.: "The SLG24 of the same S24-allele at different places are perfectly conserved in self-incompatible Brassica camprestris L" Genes Genet. Syst. 71. 255-259 (1996)
• [Publications] Higashi, K., Kamada, H. and Harada, H.: "The effects of reduced nitrogenous compounds suggests that glutamine synthetase activity is involved in the development of somatic embryos in carrot" Plant Cell Tissue Organ Culture. 45. 109-114 (1996)
• [Publications] Yang, H., Saitou, T., Komeda, Y., Harada, H. and Kamada, H.: "LEA protein in Arabidopsis thaliana homologous to carrot ECP31." Physiol. Plant.98. 661-666 (1996)
• [Publications] Yang, H., Saitou, T., Komeda, Y., Harada, H. and Kamada, H.: "AtECP63 cDNA that encodes a LEA protein belonging to group 3 is located on chromosome 4 of Arabidopsis thaliana (L) Heynh." Gene. 184. 83-88 (1996)
• [Publications] Torii et al, komeda: "The Arabidopsis ERECTA gene encodes a putative receptor protein kinase with extracellular leucine-rich repeats." Plant Cell. 8. 735-735 (1996)
• [Publications] Suzuki et al, Komeda: "Structural and functional characterization of the intergenic spacer region of the rDNA in Daucus carota." Plant Cell Physiol.37・2. 233-238 (1996)
• [Publications] Nagasawaetal, Nagato: "Mutations associated with floral organ number in rice." Planta. 198. 627-633 (1996)
• [Publications] Matsuda et al, Toriyama: "Partial male steriliWy in transgenic tobacco carrying antisense and sense PAL cDNA under the control of tapetum-specific promoter" Plant Cell Physiol. 37. 215-222 (1996)
• [Publications] Kitasiba et al, Toriyama: "Identification of genes expressed in the shoot apex of Brassica campestris during floral transition" Sex. Plant Reprod. 15. 727-730 (1996)
• [Publications] Kubo, T. and Mikami, T.: "A duplicated sequence in sugarbeet mitochondrial transcripts is differentially edited : analysis of orfB and its derivative orf324 mRNAs." Biochimica et Biophysica Acta. 1307. 259-262 (1996)
• [Publications] Hong, S-K., Kitano, H., Satoh, H. and Nagato, Y.: "How is embryo size genetically regulated in rice ?" Development. 122. 2051-2058 (1996)
• [Publications] Sato Y., Kitano, H., Nagato, Y. et al: "A rice homeobox gene, OSH1, is expressed prior to organ differentation in a specific region during early embryogenesis." Proc. Natl. Acad. Sci. USA. 93. 8117-8122 (1996)
• [Publications] Ono, M., Kamada, H. Harada, H. et al: "Transient increase in the level of mRWA for a germin-like protein in leaves of the short-day plant Pharbitis nil during the photoperiodic induction of flowering" Plant Cell Physiol. 37. 855-861 (1996)
• [Publications] Yamakawa,S.,Watanabe,M.,Hinata,K.,Suzuki,A.and Isogai,A.: "The sequences of S-receptor Kinase(SPK)involved in self-incompatibility and their homologies to S-locus glycoprotein of Brassica campestris." Biosci.Biotech.Biochem.59. 161-162 (1995)
• [Publications] Hinata,K.,Watanabe,M.,Yamakawa,S.,Satta,Y.and Isogai,A.: "Evolutionary aspects of the S-related genes of the Brassica self-incompatibility system:synonymous and nonsynonyomous base substitutions." Genetics. 140. 1099-1104 (1995)
• [Publications] Suzuki,G.,Watanabe,M.,Toriyama,K.,Isogai,A.,and Hinata,K.: "Molecular cloning of members of the S-multigene family in self-incompatible Brassica campestris L." Plant Cell Physiol.36. 1273-1280 (1995)
• [Publications] Shiba,H.,Hinata,K.,Suzuki,A.and Isogai,A.: "Breakdown of self-incompatibility in Brassica by the antisense RNA of SLG gene." Proc.Japan Academy. 71B. 81-83 (1995)
• [Publications] Torii,KU.,Komeda,Y.et al.: "The Arabidopsis ERECTA gene encodes a putative receptor Protein kinase with extracellular leucine-rich repeats." Plant cell. (印刷中). (1996)
• [Publications] Yoshida,K.,Komeda,Y.et al.: "Heat-inducible expression system for foreign gane in cultured tobacco cells with using the HSP18.2 promoter of Arabidopsis thaliana" Applied Microbial.Biotechnology. (印刷中). (1996)
• [Publications] Hirano,H.Komeda,Y.et al: "Tissue-dependent expression of the rice wx^+ gene promoter in transgenic rice and petunia." Plant Cell Physiol.36. 37-44 (1995)
• [Publications] Tsukaya,H.,Inaba-Higano,K.and Komeda,Y.: "Phenotypic characterization andmolecular mapping of an acaulis2 mutant of Arabidopsis thaliana with flower stalks of much reduced length" Plant Cell.Physiol.36. 239-246 (1995)
• [Publications] Naito,S.,Komeda,Y.et al.: "Expression of sybean aseed storage protein genes in transgenic plants and their response to sulfur nutritional conditions." J.Plant Physiol.145. 614-619 (1995)
• [Publications] Suzuki,A.,Tanifuji,S.,Komeda,Y.and Kato,A.: "three protein fraactions that bind to a narrow region of the promoter of the ribosomal RNA gene in Vicia faba" Plant Cell Physiol.36. 1051-1058 (1995)
• [Publications] Sakamoto,K.,Komeda,K.,Kamada,H. et al: "A male-associated DNA sequence in a dioecious plant,Cannabis sativa L." Plant Cell Physiol.36. 1549-1554 (1995)
• [Publications] Higashi,K.,Kamada,H.and Harada,H.: "The effects of reduced nitrogenous compounds suggests that glutamine synthetase isincolved in the development of somatic embryos in carrot" Plant Cell Tissue Organ Culture. (印刷中). (1996)
• [Publications] Kishima,Y.Mikami,T et al: "Chloroplast DNA analysis in buckwheat species: Phylogenetic relationships,origin of the reproductive systems and extended inverted repeats" Plant Science. 108. 173-179 (1995)
• [Publications] Kishima,Y.Yanai,Y.,Kinoshita,T.and Mikami,T.: "Physical mapping of differences between the chloroplast DNAs of Beta vulgaris and B.webbiana." Plant Breeding. 114. 361-362 (1995)
• [Publications] Nagasawa,N.,Nagato,Y.et al: "Mutations associated with Floral organ number in rice" Planta. (印刷中). (1996)
• [Publications] Toriyama,K.et al: "A cDNA clone encoding an IgE-binding protein from Brassica anther has significant sequence similarity to Ca2^<+->biding proteins" Plant Mol.Biol.(印刷中). (1996)
• [Publications] N.Matsuda,K.Toriyama et al: "Partial male sterility in transgenic tobacco carrying antisense and sense PAL cDNA under the control of tapetum-specific promoter" Plant cell Physiol. (印刷中). (1996)
• [Publications] Tsuchiya,T.,K.Toriyama,et al: "Tapetum-specific expression of the gene for an endo-β-1,3-glucanase causes male sterility in transgenic tobacco" Plant Cell Physiol. 36. 487-494 (1995)
• [Publications] Sage-Ono,K.,Kamada,H.et al: "Modification of Gene Exprression and Non-Mendelian Inheritance" Cloning and expression of cDNAs relating to photoperiodic floral induction in Pharbitis nil Choisy cv.Violet,
• [Publications] 三好正治・長戸康郎: "種子のバイオサイエンス" 花の形成, 5-9 (1995)