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Molecular and Cellular Biology of Tobacco BY-2 Cell Line

Research Project

Project/Area Number 07304053
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section総合
Research Field 植物生理
Research InstitutionUniversity of Tokyo

Principal Investigator

NAGATA Toshiyuki  Graduate School of Science, Professor, 大学院・理学系研究科, 教授 (10012519)

Co-Investigator(Kenkyū-buntansha) NISHITANI Kazuhiko  College of Liberal Arts, Kagoshima University, Assoc.Professor, 教養部, 助教授 (60164555)
SONOBE Seiji  Faculty of Science, Himeji Institute of Technology, Assoc.Professor, 理学部, 助教授 (30197024)
HASEZAWA Seiichiro  Graduate School of Science, Assoc.Professor, 大学院・理学系研究科, 助教授 (40172902)
NAKAMURA Kenzo  Faculty of Agriculture, Nagoya University, Professor, 農学部, 教授 (80164292)
SHIBAOKA Hiroh  Faculty of Science, Osaka University, Professor, 理学部, 教授 (60087054)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥11,400,000 (Direct Cost: ¥11,400,000)
Fiscal Year 1996: ¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 1995: ¥6,000,000 (Direct Cost: ¥6,000,000)
Keywordstobacco BY-2 cells / cell synchronization / cytoskeletons / auxin / cytokinin / in vitro transcription system / miniprotoplast / 細胞増殖 / インビトロ翻訳系 / 植物ホルモン
Research Abstract

This project has been intended and conducted to facilitate the acceleration of research progress and information change on the study of molecular and cellular biology of tobacco BY-2 cell line. This subject is quite unique, since this is only one available highly synchronized system in the world that has been developed by the principal investigator and has been distributed to more than 100 laboratories in at least 20 countries. This is indispensable in particular for the cell cycle studies and is being used in major laboratories in the world. Thus' it was intended in this study to facilitate the informational exchange and romote the progress of scientific studies using this cell line in this Country as well.
As aconsequence after two years' efforts, it was shown by using highly synchronized cell populations that molecular mechanism of the organization of cortical microtubules has been clearly demonstrated and several types of kinesin molecules oscillated differently during cell cycle pr … More ogression. It is also revealed that a novel auxin-signal transduction chain is involved in the maintanance of cell division activity in tobacco BY-2cells. Furthermore, the development of a novel in vitro transcription system by one of collaborators using BY-2 cells facilitated further the popularity of this cell line at the international level. It was also shown that there are two different transport systems of proteins into vacuoles that are dependent on unique sequence in the C-terminal and N-terminal sequences of proteins, respectively. Conversion of proplastids to amyloplasts upon the treatment by cytokinin has also been analyzed by one investigator. Thus, it may be said that even within the short frame of two years significant progress of the understanding of the cellular and molecular biology on tobacco BY-2 has been attained, resulting in understanding the basic phenomenon of plant cells in general.
In 1995, a public forum was held at the occasion of Annual Meeting of the Botanical Society of Japan to familiarize the topics of discussion in this group to much wider circles in this country. Responses from the audiences were quite significant, since some of these peoples would like to introduce this experimental system in their studies. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Kumagai,F.et al.: "The involvement of protein synthesis elongation factor 1 α in the organization of microtubules on the perinuclear region during the cell cycle transition from M phase to G_1 phase in tobacco BY-2 cells." Botanica Acta. 108. 467-473 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mitsui,H.et al.: "Cell-cycle dependent accumulation of a kinesin-like protein,KatB/C,in synchronized tobacco BY-2 cells." Plant Mol.Biol.30. 177-181 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kusaba,M.et al.: "A multiple-stimuli-responsive as-1-related element of parA gene confers responsiveness to cadmium but not copper." Plant Physiol.111. 1161-1167 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shibaoka,H et al.: "The use of model systems prepared from tobacco BY-2 cells for studies of the plant cytoskeleton." J.Microscopy. 181. 145-152 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuoka,K.et al.: "Different sensitivity to wortmannin of two vacuolar sorting signals indicated the presence of distinct sorting machineries in tobacco cells." J.Cell Biol.130. 1307-1318 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sakai,A.et al.: "Amyloplast formation in cultured tobacco cells ; effects of plant hormones on multiplication,size,starch content." Plant Cell Reports. 15. 601-605 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 長田敏行・内宮博文(編): "植物の遺伝子発現" 講談社サイエンティフィク, 238 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kumagai, F., Hasezawa, S., Takahashi, Y.and Nagata, T: "The involvement of protein synthesis elongation factor 1alpha in the organization of microtubules on the perinuclear region during the cell cycle transition from M phase to G_1 phase in tobacco BY-2 cells." Botanica Acta. 108. 467-473 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishida, T., Takahashi, Y.and Nagata, T: "The mode of expression and promoter analysis of the arcA gene, an auxin-regulated gene in tobacco BY-2 cells." Plant Cell Physiol.37. 439-448 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kusaba, M., Takahashi, Y.and Nagata, T: "A multiple-stimuli-responsive as-l-related element of parA gene confers responsiveness to cadmium but not copper." Plant Physiol. 111. 1161-1167 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mitsui, H., Hasezawa, S., Nagata, T.and Takahashi, H: "Cell cycle-dependent accumulation of a kinesin-like protein, Kat B/C,in synchronized tobacco BY-2 cells." Plant Mol.Biol.30. 177-181 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shibaoka, H., Asada, T., Yamamoto, S.and Sonobe, S.: "The use of model systems prepared from tobacco BY-2 cells for the studies of the cytoskeleton." J.Microscopy. 181. 14-12 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuoka, K., Bassham, D.C., Raikhel, N.V.and Nakamura, K.: "Different sensitivity ot wortmannin of two vacuolar sorting signals indicates the process of distinct sorting machineries in tobacco BY-2 cells." J.Cell Biol.130. 1307-1318 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasuhara, H., Sonobe, S.and Shibaoka, H.: "Effects of brefeldin A on the formation of the cell plate in tobacco BY-2 cells." Eur.J.Cell Biol.66. 274-281 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fan, H., Yakura, K., Miyanishi, M., Sugita, M.and Sugiura, M.: "In vitro transcription of plant RNA polymerase I-dependent rRNA genes is species-specific." Plant J. 8. 295-298 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishida,S.et al.: "The mode of expression and promoter analysis of the arcA gene,an auxin-regulated gene in tobacco BY-2 cells." Plant Cell Physiol.37. 439-448 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Asada,T.et al.: "TKRP125,a kinesin-related protein involved in the centrosome-independent organization of the cytokinetic appraratus in tobacco BY-2 cells." J.Cell Sci.110(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hanano,S.et al.: "Isolation of a novel RNA-binding protein and its association with a large ribonulceoprotein particle present in the nucleoplasm of tobacco cells." Plant Mol.Biol.31. 57-68 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sonobe S.et al.: "Studies on the plant cytoskeleton using miniprotoplasts of tobacco BY-2 cells." J.Plant Res.109. 437-448 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Suehara,K.et al.: "Optimal expression of GUS gene from methyl jasmonate-inducible promoter in high density culture of transformed tobacco cell line BY-2." J.Ferm.Bioengin.82. 51-55 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sakai,A.et al.: "Amyloplast formation in cultured tobacco cells;effects of plant hormones on multiplication,size,and starch content." Plant Cell Reports. 15. 601-605181 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takahashi, Y. et al.: "Identification of auxin-responsive elements of parB and their expression in apices and root." Proc. Natl. Acad. Sci. USA. 92. 6359-6363 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takahashi, Y. et al.: "Expression of the auxin-regulated parA gene in transgenic tobacco and nuclear localization of its gene products." Planta. 196. 111-117 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fan, H. et al.: "In vitro transcription of plant RNA polymerase I-dependent rRNA genes is speciesspecific." Plant J.8. 295-298 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kumagai, F. et al.: "The involvement of protein synthesis elongation factor 1α in the organization of microtubules on the perinuclear region during the cell cycle transition from M phase to G_1 phase in tobacco BY-2 cells." Bot. Acta. 108. 476-481 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yasuhara, H. et al.: "Effects of brefeldin A on the formation of the cell plate in tobacco BY-2 cells." Eur. J. Cell Biol.66. 274-281 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Mayumi, K. & Shibaoka, H.: "A possible double role for brassinolide in the reorientation of cortical microtubules in the epidermal cells of azuki bean epicotyls." Plant Cell Physiol.36. 173-181 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 長田敏行、内宮博文(編): "植物の遺伝子発現" 長田敏行、内宮博文(編), 238 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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