• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study on the mechanism of silkworm resistancea against diseases.

Research Project

Project/Area Number 07306004
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 蚕糸・昆虫利用学
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

HUKUHARA Toshiko  Tokyo University of Agriculture and Technology, Faculty of Agriculture, Professor, 農学部, 教授 (70011880)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Michihiro  Nagoya University Faculty of Agriculture, Professor, 農学部, 教授 (60111837)
SIMIZU Susumu  Kyoto University of Textile, Faculty of Textile, Professor, 繊維学部, 教授 (20187454)
SEKIJIMA Yasutaka  Saitama Junior College of Hygiene, 教授 (00104985)
IIZUKA Toshihiko  Hokkaido University, Faculty of Agriculture, Professor, 農学部, 教授 (50001441)
KAWARABATA Isamu  Kyushu University, Faculty of Agriculture, Professor, 農学部, 教授 (60038221)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥13,400,000 (Direct Cost: ¥13,400,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1995: ¥7,700,000 (Direct Cost: ¥7,700,000)
Keywordsentomopoxvirus / persitent infection / microsporidia / Bacillus thuringiensis / Paecilomyces fumoso-roseus / unclear polyhedrosis virus / Bombyx mori / Pseudaletia separata / 顆粒病ウイルス / 黒きょう病菌 / ウイルス / 細菌 / 真菌 / 補体 / 耐病性 / 免疫
Research Abstract

A Pseudaletia separata entomopoxvirus gene coding for a virus enhancing factor was cloned and sequaenced. It was highly homologous with fusolin genes. The factor promoted the fusion of the envelope of a nuclear polyhedrosis virus with the cell plasma membrane of an insect cell line that had been derived from the hemocyte of P.separata. When several establilshed lepidopteran cell lines that had been infected with microsporidia were passaged, persistantly infected cultures were obtained due to the production, by the infected cells, of a factor inhibitory for the growth of the sporoplasm. A strain of Bacillus thuringiensis from Indonesian soil produced three morphologically different toxic crystals. The toxic polypeptides were 130 and 65 kDa in molecular mass and caised death of lepidopteran insects. A factor which inhibited C3b activation in the alternate pathway of the human complement system was isolated from silkworm hemolymph. An entomogenous fungus, Paecilomyces fumoso-roseus, produced a protease which might play an important role in invasion via the integument. The midgut epithelium of the silkworm produced 45.5 and 44 kDa polypeptides when infected with a densonucleosis virus(type2). They appearaed to be a secondary product associated with cellular death because they were not coded by the virus genome.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Chaeychomsri, S.: "Temporal regulation of Bombyx mori(Lepidoptera:Bombycidae)nucleopolyhedrovirus UNA polymerase gene expression." Appl.Entomol.Zool.32. 418-421 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sotoshiro, H.: "Identification of a novel virus-specific in vitro translation product in the midgut of the silkworm,Bombyx mori,infected with B.mori densovirus type 2." J.Sericult.Sci.Jpn.66. 38-47 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tomita, M.: "Serological relationship between inclusion body proteins and a virus enhancing facor of an entomopoxvirus." Appl.Entomol.Zool.33(印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Wijonarko, A.: "Detection of a virus enhancing factor in the spheroid,spindle and virion of an entomopoxvirus." J.Invertebr.Pathol.97(印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 横田 仁子: "鱗翅目昆虫病原性微胞子虫Nosema mesnili NM-HC-A8801株のAntheraea eucalypti細胞系への接種と増殖." 応動昆. 41. 89-94 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 横田 仁子: "数種鱗翅目昆虫培養細胞系における微胞子虫Nosema mesnili NM-HC-A8801株の感染・増殖および持続感染系の樹立." 応動昆. 41. 147-152 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 福原 敏彦: "昆虫伝染病の科学史" サイエンスハウス, 151 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Chaeychomsri, S., Ikeda, M., and Kobayashi, M.: "Nucleptide sequence and transcriptional analysis of the DNA polymerase gene of Bombyx mori unclear polyhedrosis virus." Virology. 206. 435-447 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Chaeychomsri, S., Ikeda, M., and Kobayashi, M.: "Expression of viral DNA polymerase gene in the larvae of the silkworm, Bombyx mori(Lepidoptera : Bombycidae), during the infection with unclear polyhedrosis virus." Appl.Entomol.Zool.30. 451-465 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Chaeychomsri, S., Ikeda, M., and Kobayashi, M.: "Temporal regulation of Bombyx mori(Lepidoptera : Bombycidae)uncleopolyhedrovirus DNA polymerase gene expression." Appl.Entomol.Zool.32. 418-421 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hayakawa, T., Xu, J., and Hukuhara, T.: "Cloning and sequencing of the gene for an enhancing factor from Pseudaletia separata entomopoxvirus." Gene. 177. 269-270 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hukuhara, T., Yano, K., Xu, J., Tomita, M., and Miyajima, S.: "Detection of a virus enhancing factor in inclusion bodies of an entomopoxvirus by immunoelectron microscopy." J.Invertebr.Pathol.65. 315-317 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sotoshiro, H., Ikeada, M., and Kobayashi, M.: "Comparative analysis of Bombyx mori(Lepidoptera : Bombycidae)densovirus type 2 polypeptides synthesized in vivo and in vitro." Appl.Entomol.Zool.31. 154-159 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sotoshiro, H., Ikeda, M., and Kobayashi, M.: "Indentification of a novel virus-specific in vitro translation product in the midgut of the silkworm, Bombyx mori, infected with B.mori densovirus." J.Sericult.Sci.Jpn. 66. 38-47 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tomita, M., Takahashi, Y., Hukuhara, T., and Miyajima, S.: "Serological relationship between inclusion body proteins and a virus enhancing facor of an entomopoxvirus." Appl.Entomol.Zool.(in press.). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Wijonarko, A., and Hukuhara, T.: "Detection of a virus enhancing factor in the spheroid, spindle and virion of an entomopoxvirus." J.Invertebr.Pathol.(in press.). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yano, K., Tsunekawa, S., and Hukuhara, T.: "Replication of a small isometric virus in cultured Lymantria dispar (Lepidoptera : Lymantridae) cells." Appl.Entomol.Zool.31. 637-641 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Chaeychomsri, S.: "Temporal regulation of Bombyx mori (Lepidoptera : Bombycidae) nucleopolyhedrovirus DNA polymerase gene expression." Appl.Entomol.Zool. 32. 418-421 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sotoshiro, H.: "Identification of a novel virus-specific in vitro translation product in the midgut of the silkworm, Bombyx mori, infected with B.mori densovirus type 2." J.Sericult.Sci.Jpn. 66. 38-47 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tomita,M.: "Serological relationship between inclusion body proteins and a virus enhancing facor of an entomopoxvirus." Appl.Entomol.Zool.33(in press.). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Wijonarko, A.: "Detection of a virus enhancing factor in the spheroid, spindle and virion of an entomopoxvirus." J.Invertebr.Pathol.97(in press.). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 横田仁子: "鱗翅目昆虫病原性微胞子虫 Nosema mesnili NM-HC-A8801株のAntheraea eucalypti細胞系への接種と増殖" 応動昆. 41. 89-94 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 横田仁子: "数種鱗翅目昆虫培養細胞系における微胞子虫 Nosema mesnili NM-HC-A8801株の感染・増殖および持続感染系の樹立." 応動昆. 41. 147-152 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 福原 敏彦: "昆虫伝染病の科学史" サイエンスハウス, 151 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 時連根・清水進: "Metarhizium anisopliue var.majusからのプロトプラスト作出条件の検討" 日蚕雑. 65(4). 201-204 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hayakawa,T.: "Cloning and sequencing of the gene for an enhancing factor from Pseudaletia separata entomopoxvirus" Gene. 177. 269-270 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yano,K.: "Replication of a small isometric virus in cultured Lymantria dispar (Lepidoptera : Lymantriidae) cells." Appl.Entomol.Zool.31(4). 637-641 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sotoshiro,H.: "Comparative analysis of Bombyx mori (Lepidoptera : Bombycidae) densovirus type 2 polypeptides synthesized in vivo and in vitro." Appl.Entomol.Zool.31(1). 154-159 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 佐々木潤: "Bacillus thuringiensis分離株の有するcryV遺伝子の検索" 日蚕雑. 65(1). 56-61 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 橋本直樹: "CryIA(a)遺伝子プロモーター制御下におけるBacillus thuringiensis ICP遺伝子の発現" 日蚕雑. 65(3). 185-191 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Chaeychomsri S.: "Nucleotide sequence and transcriptional analysis of the DNA polymerase gene of Bombyx mori unclear polyhedrosis virus." Virology. 206. 435-447 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Chaeychomsri S.: "Expression of viral DNA polymerase gene in the larvae of the silkworm,Bombyx mori(Lepidoptera:Bombycidae),during the infection wiht unclear polyedrosis virus." Appl.Entomol.Zool.30. 451-465 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sotoshiro H.: "Identification of viral structural polypeptides in the midgut and feces of the silkworm,Bombyx mori,infected with Bombyx densovirus type2." J.Invertebr.Pathol.66. 60-67 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sotoshiro H.: "Comparative analysis of Bombyx mori(Lepidoptera:Bombycidae)densovirus type 2 polypeptides synthesized in vivo and in vitro." Appl.Entomol.Zool.31. 154-159 (1996)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi