| Project/Area Number |
07306019
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 総合 |
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| Research Field |
蚕糸・昆虫利用学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KOBAYASHI Masahiko The University of Tokyo, Graduate school of Agricultural and life Sciences, Professor, 大学院・農学生命科学研究科, 教授 (60162020)
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| Co-Investigator(Kenkyū-buntansha) |
AGUI Noriaki National Institute of Health, Department of Medical Entomology, Director of Depa, 昆虫医科学部, 部長 (30109962)
OZAKI Masataka The University of Tokyo, Graduate school of Agricultural and life Sciences, Assi, 大学院・農学生命科学研究科, 助手 (00270893)
SHIMADA Toru The University of Tokyo, Graduate school of Agricultural and life Sciences, Asso, 大学院・農学生命科学研究科, 助教授 (20202111)
OSHIKI Toshikazu Tokyo University of Agriculture and Technology, Faculty of Agriculture, Professo, 農学部, 教授 (80015081)
DOIRA Hiroshi Kyushu University, Institute of Genetic Resources, Faculty of Agriculture, Profe, 農学部, 教授 (30038210)
山下 興亜 名古屋大学, 農学部, 教授 (50023411)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1996: ¥5,700,000 (Direct Cost: ¥5,700,000)
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| Keywords | sex determining gene / RAPD / sex chromosome / vitellogenin / egg specific protein / gynandromorph / storage protein / polyploid / 雄雌モザイク / RAPD法 / 種間雑種 |
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| Research Abstract |
1. Homologous genes of the sex determining and expressing genes of Drosophila melanogaster, Sxl, tra and dsx, were searched from the genomes of Bombyx mori, Culex tritaeniorhyncus and Musca domestica. Candidate clones of Sxl homologue were found except from B.mori, however, whether they were actually concerned in sex determination or not could not be confirmed.
2. Linkage map of Random Amplified Polymorphic DNA (RAPD) of B.morigenome was constructed and a few sex-linked and sex-limited RAPDs were found. Nucleotide sequence of these RAPDs were analyzed in order to clarify the structure of sex chromosomes.
3. Structure of genes coding proteins which express differently between sexes, such as vitellogenin and vitellogenin receptor of M.domestica, vitellogenin, egg specific protein and storage proteins of B.mori, etc., was analyzed and regulation by endocrine factors or influence of environmental conditions were investigated.
4. To investigate the mechanism of sex determination in B.mori, sexual characters of fertility were examined in strains containing translocated sex chromosome and polyploid individuals such as triploid, tetraploid or hexaploid.
5. Sex mosaic individuals (gynandromorphs) were produced in a B.mori strain which contains a mutation affecting gonadal development and structure of gonads or developmental feature of germ cells of them were examined.
6.Inter specfic hybrids between Antheraea yamamai and A.pernyiwere raised and female sterility in F1 of the crossing A.y. female x A.p. male which caused by deficient egg development was confirmed.
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