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MOLECULAR MECHANISMS OF FISH REPRODUCTION

Research Project

Project/Area Number 07306021
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section総合
Research Field General fisheries
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

YAMAUCHI Kohei  Hokkaido Univ., Fac.of Fish., Pro., 水産学部, 教授 (10109514)

Co-Investigator(Kenkyū-buntansha) MATSUYAMA Michiya  Kyusyu Univ., Fac.of Agr., Ass.Pro., 農学部, 助教授 (00183955)
NAGAHAMA Yoshitaka  Natl.Ins.Basic Biol., Pro., 基礎生物学研究所, 教授 (50113428)
TAKASHIMA Fumio  Tokyo Univ.of Fish., Pro., 資源育成学科, 教授 (60041703)
KAWAUCHI Kohji  Kitazato Univ., Fac.of Fish., Pro., 水産学部, 教授 (70050523)
KANEKO Toyoji  Tokyo Univ., Oc.Res.Ins., Instr., 海洋研究所, 助手 (70221190)
AIDA Katsumi  Tokyo Univ., Fac.of Agr., Pro., 農学部, 教授 (50012034)
ANDO Hironori  Hokkaido Univ., Fac.of Sci., Instr., 理学部, 助手 (60221743)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1996: ¥3,300,000 (Direct Cost: ¥3,300,000)
KeywordsGnRH / GTH / somatolactin / connexin / 20beta-HSD / P450c17 / estrogen receptor / molecular mechanism
Research Abstract

(Hypothalamus) The two salmon gonadotropin-releasing horman genes (sGnRH-I and sGnRH-II) and their upstream regions were characterized in masu salmon. In the upstream regions, only the sGnRH-II gene has a large palindromic sequence. In contrast, the sGnRH-I gene is missing the large palindromic sequence. Changes in sGnRH synthetic activity in the brain during gonadal maturation were also examined by in situ hybridization in masu salmon. During gonadal maturation, the numbers of neurons expressing sGnRH mRNA increased in the preoptic area and the ventral telencephalon.
(Pituitary) cDNA of II beta-subunit of gonadotropin (GTH) in Rusian stergeon was cloned. In addition, the possible involvement of somatolactin in acid-base regulation was suggested in rainbow trout.
(Gonad) The two types of cDNA encoding P-450c17 and one type of 20 beta-IISD were isolated from rainbow trout ovary. Furthermore, cDNAs encoding several types of connexins which has the important role for the acquisition of oocyte maturational competence were also isolated from the atlantic croker.
(Liver) Changes in levels of estrogen receptor gene expression in the liver were also examined by RT-PCR during gonadal maturation in eel.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (35 results)

All Other

All Publications (35 results)

  • [Publications] Hiraoka et al.: "Effects of osmotic stimulation on expression of neurohypophysial hormone genes in pre-spawning chum salmon." Zool.Sci.13. 737-745 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kakizawa et al.: "Elevation of plasma somatolactin concentrations during acidosis in rainbow trout(Oncorhynchus mykiss)." J.Exp.Biol.199. 1043-1051 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kobayashi et al.: "Steroidogenesis in the ovarian follicles of the medaka(Oryzias latipes)during vitellogenesis and oocyte maturation." Zool.Sci.13. 921-927 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Todo et al.: "Molecular cloning and characterization of Japanese eel estrogen receptor cDNA." Mol.Cell.Endocrinol.119. 37-45 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kakizawa et al.: "Effects of hypothalamic factors on somatolactin secretion from the organ-cultured pituitary of rainbow trout." Gen.Comp.Endocrinol.105. 71-78 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Amano et al.: "Distribution and function of gonadotropin-releasing hormone(GnRH)in the teleost brain." Zool.Sci.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Amano et al.: "Activation of salmon GnRH mRNA expression prior to differentiation of precocious males in masu salmon." Gen.Comp.Endocrinol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Amano et al.: "The maturation of the salmon GnRH system and its regulation by gonadal steroids in masu salmon." Fish Physiol.Biochem.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiraoka et al.: "Changes in expression of neurohypophysial hormone genes during spawning migration in chum salmon.Oncorhynchus keta." J.Mol.Endocrinol.(in Press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiraoka et al.: "Effects of osmotic stimulation on expression of neurohypophysial hormone genes in pre-spawning chum salmon." Zool.Sci.13. 737-745 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kakizawa et al.: "Elevation of plasma somatolactin concentrations during acidosis in rainbow trout (Oncorhynchus mykiss)." J.Exp.Biol.199. 1043-1051 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kobayashi et al.: "Steroidogenesis in the ovarian follicles of the medaka (Oryzias latipes) during vitellogenesis and oocyte maturation." Zool.Sci.13. 921-927 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Todo et al.: "Molecular cloning and characterization of Japanese eel estrogen receptor cDNA." Mol.Cell.Endocrinol.119. 37-45 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kakizawa et al.: "Effects of hypothalamic factors on somatolactin secretion from the organ-cultured pituitary of rainbow trout." Gen.Comp.Endocrinol.105. 71-78 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Amano et al.: "Distribution and function of gonadotropin-releasing hormone (GnRH) in the teleost brain." Zool.Sci.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Amano et al.: "Activation of salmon GnRH mRNA expression prior to differentiation of precocious males in masu salmon." Gen.Comp.Endocrinol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Amano et al.: "The maturation of the salmon GnRH system and its regulation by gonadal steroids in masu salmon." Fish Physiol.Biochem.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiraoka et al.: "Changes in expression of neurohypophysial hormone genes during spawning migration in chum salmon, Oncorhynchus keta." J.Mol.Endocrinol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiraoka et al.: "Effects of osmotic stimulation on expression of neurohypophysial hormone genes in pre-spawning chum salmon." Zool. Sci.13. 737-745 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kakizawa et al.: "Elevation of plasma somatolactin concentrations during acidosis in rainbow trout (Oncorhynchus mykiss)." J. Exp. Biol.199. 1043-1051 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kobayashi et al.: "Steroidogenesis in the ovarian follicles of the medaka (Oryzias latipes) during vitellogenesis and oocyte maturation." Zool. Sci.13. 921-927 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Todo et al.: "Molecular cloning and characterization of Japanese eel estrogen receptor cDNA." Mol. Cell. Endocrinol.119. 37-45 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kakizawa et al.: "Effects of hypothalamic factors on somatolactin secretion from the organ-cultured pituitary of rainbow trout." Gen. Comp. Endocrinol.105. 71-78 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Amano et al.: "Distribution and function of gonadotropin-releasing hormone (GnRH) in the teleost brain." Zool. Sci.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Amano et al.: "Activation of salmon GnRH mRNA expression prior to differentiation of precocious males in masu salmon." Gen. Comp. Endocrinol.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Amano et al.: "The maturation of the salmon GnRH system and its regulation by gonadal steroids in masu salmon." Fish Physiol. Biochem.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Hiraoka et al.: "Changes in expression of neurohypophysial hormone genes during spawning migration in chum salmon, Oncorhynchus keta." J. Mol. Endocrinol.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Amano et al.: "Salmon GnRH synthesis in the preoptic area and the ventral telencephalon is activated during gonadal maturation in female masu salmon." Gen.Comp.Endocrinol.90. 13-24 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kim et al.: "Immunocytochemical localization of sGnRH and cGnRH-II in the brain of gold fish,Carasius auratus." J.Comp.Neurol.356. 72-82 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ashihara et al.: "Two differing precursor genes for the salmon-type gonadotropin-releasing hormone exist in salmonids." J.Mol.Endocrinol.15. 1-9 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kakizawa et al.: "Changes in plasma somatolactin levels during spawning migration of chum salmon(Oncorhynchus keta)." Fish Physiol.Biochem.14. 419-425 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nishll et al.: "Isolation and characterization of [Po^2]-somatostatin-14 from Russian sturgeon,Acipenser gueldenstaedtl." Gen.Comp.Endocrinol.99. 6-12 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tanaka et al.: "Structure and promoter analysis of the cytochrome P-450 aromatase gene of the teleost fish,Medaka(Oryzias latipes)." J.Biochem.117. 719-725 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Okumura et al.: "Development of sensitive sandwitch enzyme-linked immunosorbent assay(ELISA)for vitellogenin in the Japanese eel,Anguilla japonica." Fisheries Sci.61. 283-289 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Todo et al.: "Hepatic estrogen receptors in the Japanese eel,Anguilla japonica:Characterization and changes in binding capacity during artificially-induced sexual maturation." Zool.Sci.12. 789-794 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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