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Study of signal transduction via an SH2 which recognizes phosphotyrosine residues of proteins

Research Project

Project/Area Number 07307003
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section総合
Research Field General medical chemistry
Research InstitutionKOBE UNIVERSITY

Principal Investigator

YAMAMURA Hirohei  Kobe University School of Medicine Professor, 医学部, 教授 (90030882)

Co-Investigator(Kenkyū-buntansha) OKADA Masato  Osaka University Insitute for protein Research Associate Professor, 蛋白質研究所, 助教授 (10177058)
NOZAWA Yoshinori  Gifu University School of Medicine Professor, 医学部, 教授 (10021362)
YAMAMOTO Tadashi  University of Tokyo Institute of Medical Science Professor, 医科学研究所, 教授 (40134621)
ONO Yoshitaka  Kobe University Faculty of Science Professor, 理学部, 教授 (10243297)
MATOZAKI Takashi  Kobe University School of Medicine Research Associate, 医学部付属病院, 助手 (80252782)
武藤 多津郎  福井医科大学, 医学部, 助手 (60190857)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 1996: ¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1995: ¥6,800,000 (Direct Cost: ¥6,800,000)
KeywordsSyk / protein-tyrosine kinase / Lyn / CD45 / DT-40 cell / SHP-2 / protein-tyrosine phosphatase / SHPS-1 / SHp-2 / チロシンホスファターゼ / パキシリン / SH-PTP2 / CSk / ZAP-70 / Trk
Research Abstract

SH2 (src homology region 2) is a region which specifically recognized the phosphotyrosine residues in various proteins and is believed to be an important structure in signal transduction. A non-receptor type protein-kinase Syk is expressed in almost all the hematopoietic cells. We tried to found out the importance of 2 SH2 of Syk in signal transduction. Both SH2 domains were required for BCR-mediated Syk and phospholipase C_<gamma>2 phosphorylation, inositol 1,4,5-triphosphate release and calcium mobilization. A possible explanation for this requirement was provided by findings that recruitment of Syk to tyrosine-phosphorylated immuno-globulin a and reqiures both Syk SH2 domains.
Protein tyrosine phosphatase, such as SHP-1 AND SHP-2, that contain SH2 domains play important roles in growth factor and cytokine signal transduction pathways. A protein of -115 kD that interacts with SHP-1 and SHP-2 was purified from v-src-transformed rat fibroblasts and the corresponding cDNA was cloned (SHP … More S-1). It has four YXX (L/V/I) motifs, potential tyrosine phosphorylation and SH2-domain binding sites, in its cytoplasmic region. SHP-1 may be a potential substrate for SHP-2 and may function in both growth factor-and cell adhesion-induced cell signaling.
Interaction of Fyn and Zap-70 in T cells was studied. Deletion of both the SH2 and SH3 domains of Fyn resulted in the decrease of the assciation with Zap-70. Consistently, Fyn-SH2 and SH3 fused to glutathione S-transferase were able to bind to Zap-70. Thus multiple sites of Fyn and Zap-70 are involved in the association. We propose that Fyn phosphorylates and activates Zap-70 and that both kinases cooperate in TCR signaling.
Tyrosine phosphorylation of paxillin was induced by nerve growth factor and epidermal growth factor and KCI.Various evidence suggested that tyrosine phosphorylation of paxillin may be involved in calcium-dependent events in neuronal and neuroendocrine cells.
We have succeeded in making a CD45-deficient DT-40 cells. Using this cell lines we have found that the dephosphorylation of tyrosine residues at both autophosphorylation and negative regulatory sites is mediated by CD45 in vivo and that dephosphorylation of C-terminal tyrosine is a prerequisite for participation of Lyn in B cell receptor signaling.
We have been searching the family of Syk/Zap-70 in brain and other tissues. We found novel kinases in brain and liver cells. Now we are purifying these kinases and cloning the cDNAs of these kinases. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Maruyama, S. et. al.: "Physical and functional association of cortactin with Syk in human leukemic cell line K562." J. Biol. Chem.271(12). 6631-6635 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T, Noguchi. et. al.: "Characterization of a 115-kDa protein that binds to SH-PTP2, a protein-tyrosine phosphatase with SRC homology 2 domains, in Chinese hamster ovary cells." J. Biol. Chem.271(44). 27645-27651 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mukai, H. et. al.: "Translocation of PKN from the cytosol to the nucleus induced by stresses." Proc. Natl. Acad. Sci. U. S. A.93. 7639-7643 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T, Tezuka. et. al.: "Physical and functional association of the Cbl proto-oncogene product with a Src-family protein-tyrosine kinase, p53/p561yn, in the B-cell antigen receptor-mediated signaling." J. Exp. Med.183. 675-680 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S. Yoshimura. et. al.: "Differential translocation of phospholipase C isozymes to integrin-mediated cytoskeletal complexes in thrombin-stimulated human platelets." Biochem. Biophys. Res. Commun.225. 494-499 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tamaru, T. et. al.: "Purification and Characterization of a p13suc1-Bound Serine/Threonine Kinase Expressed in Mature Rat Brain" Eur. J. Biochem.283. 152-159 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kurosaki, T., Johnson, S.A., Sada, K., Yamamura, H.and Cambier, J.C.: "Syk recruitment to Ig-a and its phosphorylation are essential for B cell antigen receptor signaling." J.Exp.Med.182. 1815-1823 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Maruyama, S., Kurosaki, T., Sada, K., Yamanashi, Y., Yamamoto, T., and Yamamura, H.: "Physical and functional association of cortactin with Syk in human leukemic cell line K562." J.Biol.Chem.271(12). 6631-6635 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Noguchi, T.Matozaki, Y.Fujioka, T.Yamao, M.Tsuda, T.Takada, M.Kasuga.: "Characterization of a 115kDa protein that binds to SH-PTP2, a protein-tyrosine phosphatase with SRC homology 2 domains, in Chinese hamster ovary cells." J.Biol.Chem.271. 27645-27651 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Fujioka, T.Matozaki, T.Noguchi, A.Iwamatsu, T.Yamao, N.Takahashi, M.Tsuda, T.Takada, M.Kasuga.: "A novel membrance glycoprotein, SHPS-1, that binds the SH2 domain-containing protein tyrosine phosphatase SHP-2 in response to mitogens and cell adhesion." Mol.Cell.Biol.16. 6887-6899 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Konishi, H., Matsuzaki, H., Tanaka, M., Ono, Y., Tokunaga, C., Kuroda, S., and Kikkawa, U.: "Activation of RAC-protein kinase by heat shock and hyperosmolarity stress through a pathway independent of phosphatidylinositol 3-kinase." Proc.Natl.Acad.Sci.U.S.A.93. 7639-7643 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mukai, H., Miyahara, M., Sunakawa, H., Shibata, H., Toshimori, M., Kitagawa, M., Shimakawa, M., Takanaga, H., and Ono, Y.: "Translocation of PKN from the cytosol to the nucleus induced by stresses." Proc.Natl.Acad.Sci.U.S.A.93. 10195-10199 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Tezuka, H.Umemori, N.Fusaki, T.Yagi, M.Takata, T.Kuroaki, and T.Yamamoto.: "Physical and functional association of the Cb1 proto-oncogene product with a Src-family protein-tyrosine kinase, p53/p56lyn, in the B-cell antigen receptor-mediated signaling." J.Exp.Med.183. 675-680 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Yamanashi, T.Fukuda, H.Nishizumi, T.Inazu, K.Higashi, D.Kitamura, T.Ishida, H.Yamamura, T.Watanabe, and T.Yamamoto.: "Role of tyrosine phosphorylation of HS1 in B-cell antigen receptor-mediated signaling." J.Exp.Med.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Yoshimura, S.Nakashima, K.Ohguchi, H.Sakai, J.Shinoda, N.Sakai and Y.Nozawa: "Differentail mRNA expression of phospholipase D(PLD) isozymes during cAMP-induced differentiation in C6 glioma cells." Biochem.Biophys.Res.Commun.225. 494-499 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Banno, S.Nakashima, M.Ohzawa and Y.Nozawa: "Differential translocation of phospholipase C isozymes to integrin-mediated cytoskeletal complexes in thrombin-stimulated human platelets." J.Biol.Chem.271. 14989-14994 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tamaru, T., Okada, M.: "Purification and Characterization of a p13suc1-Bound Serine/Threonine Kinase Expressed in Mature Rat Brain" Eur.J.Biochem.283. 152-159 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kanazawa, S., Ilic Dusko, Hashiyama, M., Okada, M., Nomura T., Aizawa, S., and Suda, T.: "Impaired development of CD4+CD8+ thymocytes by csk-"knock-in"into fyn locus" Oncogene. 13. 199-204 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Maruyama,S.et.al.: "Physical and functional association of cortactin with Syk in human leukemic cell line K562." J.Biol.Chem.271(12). 6631-6635 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T,Noguchi.et.al.: "Characterization of a 115-kDa protein that binds to SH-PTP2,a protein-tyrosine phosphatase with SRC homology 2 domains,in Chinese hamster ovary cells." J.Biol.Chem.271(44). 27645-27651 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mukai,H.et.al.: "Translocation of PKN from the cytosol to the nucleus induced by stresses." Proc.Natl.Acad.Sci.U.S.A.93. 7639-7643 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T,Tezuka.et.al.: "Physical and functional association of the Cbl proto-oncogene product with a Src-family protein-tyrosine kinase,p53/p56lyn,in the B-cell antigen receptor-mediated signaling." J.Exp.Med.183. 675-680 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] S.Yoshimura.et.al.: "Differential mRNA expression of phospholipase D (PLD) isozymes during cAMP-induced differentiation in C6 glioma cells." Biochem.Biophys.Res.Commun.225. 494-499 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tamaru,T.et.al.: "Purification and Characterization of a p13suc1-Bound Serine/Threonine Kinase Expressed in Mature Rat Brain" Eur.J.Biochem.283. 152-159 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nagai, K. et. al.: "Tyrosine phosphorylation of She in mediated through Lyn and Syk in B cell receptor signaling." J. Biol. Chem.270(12). 6824-6829 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yanagi, S. et. al.: "The structure and function of p72syk expressed in hematopoietic cells" Cellular Signalling. 7(3). 185-193 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Qin, S. et. al.: "Activation and tyrosine phosphorylation of p72syk as well as calcium mobilization after hydrogen peroxide stimulation in peripheral blood lymphocytes." Biochem. J.308(1). 347-352 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yamamura, H.: "Syk Spleen tyrosine kinase (vertebrates)" The Protein Kinase Factsbook. 2. 109-111 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yang, C. et. al.: "Syk and Lyn are involved in radiation - induced signaling, but inactivation of Syk or Lyn alone is not sufficient to prevent radiation - induced apoptosis." J. Biochem.118(1). 33-38 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Asahi, M. et. al.: "Cyclic AMP-elevating agents negatively regulate the activation of p72syk in N-formyl - methionyl - leucyl - phenylalanine receptor signaling." Biophys. Biochem. Res. Commun.212(3). 887-893 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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