| Project/Area Number |
07307004
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 総合 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Niigata University |
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Principal Investigator |
MITSUYAMA Masao Niigata University, School of Medicine, Professor, 医学部, 教授 (10117260)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Haruo National Institute of Health, Department of Bacteriology, Head, 細菌部, 部長 (70142130)
YOSHIDA Shin-ichi University of Occupational and Environmental Health, Professor, 教授 (60128113)
YOSHIKAI Yasunobu Nagoya University, School of Medcine, Professor, 医学部, 教授 (90158402)
HIMENO Kunisuke Tokushima University, School of Medicine, Professor, 医学部, 教授 (50112339)
EZAKI Takayuki Gifu University School of Medicine, Professor, 医学部, 教授 (90151977)
矢野 明彦 長崎大学, 医学部, 教授 (20135122)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥8,300,000 (Direct Cost: ¥8,300,000)
Fiscal Year 1996: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1995: ¥4,600,000 (Direct Cost: ¥4,600,000)
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| Keywords | Intracellular parasitism / Bacteria / Protozoa / Macrophages / Infection / Host defense |
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| Research Abstract |
Several bacterial and protozoan parasites are capable of escaping the intracellular killing of phagocytes and able to multiply inside macrophages. Using intracellular parasites including Mycobacterium tuberculosis, Listeria monocytogenes, Salmonella spp., Legionella pneumophila, Yersinia spp., Toxoplasma gondii and Leishmania major, we have attempted to analyze the molecular mechanisms of intracellular prasitism and the immunological responses to those parasitic microorganisms with special reference to the expression of host defense against infection in the host.
In the present study, genes and proteins involved in the escape from phagocyte killing could be identified in some species of bacteria. Some kinds of endogenous cytokines were revealed to be important in the activation of macrophages and induction of antigen-specific T cell-dependent protective immunity. In a model of protozoan infection, analysis on the relationship between the induction of heat-shock protein and the expression of protective immunity resulted in the further understanding of the mechanism of defense.
The results obtained in this project will be useful in the up-to-date understanding of the host-parasite relationship and may pave the way to the development of novel vaccine against intracellular parasites in the future.
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