| Project/Area Number |
07307023
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 総合 |
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| Research Field |
Biological pharmacy
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HORIKOSHI Masami The University of Tokyo, Institute of Molecular and Cellular Biosciences, Associate Professor, 分子細胞生物学研究所, 助教授 (70242089)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMADA Kazuo The University of Tokyo, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (70196476)
SATO Yoshinori The University of Tokyo, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (30150014)
NATORI Shunnji The University of Tokyo, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (50012662)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥23,200,000 (Direct Cost: ¥23,200,000)
Fiscal Year 1996: ¥9,700,000 (Direct Cost: ¥9,700,000)
Fiscal Year 1995: ¥13,500,000 (Direct Cost: ¥13,500,000)
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| Keywords | Transcriptional regulatory factor / Basal transcription factor / Interaction between DNA and transcription factors / Transcription elongation factors / Transcriptional regulation / domain structure / S-II / RT-PCR method / TFIIB |
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| Research Abstract |
In order to elucidate the significance of function and structure of tissue-specific transcription clongation factor S-II family, we tried to isolate the various S-II family and to analyze the structure-function relationships by several methods.
1) Isolation and characterization of tissue-specific S-II family from various organs.
Human testis-specific S-II was isolated through the interaction with TFIIB by two-hybrid systems and three types of tissue-specific S-II family are obtained from mouse and rat by RT-RCR method. It was found that expression of mouse testis-specific S-II is restricted to testicular germ cells during and after meiosis in the course of spermatogenesis.
2) Genetical analysis of structure and function of S-II family.
To elucidate the in vivo function of S-II,we tried to set up S.pombe system. Moreover, it was found that there are three other S-II family members from S.cerevisiae genomes.
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