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Biochemical functions and metabolisms of D-amino acid

Research Project

Project/Area Number 07308048
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionKansai University (1996-1997)
Kyoto University (1995)

Principal Investigator

SODA Kenji  Kansai University, Faculty of Engineering, Professor, 工学部, 教授 (30027023)

Co-Investigator(Kenkyū-buntansha) MORIGUCHI Mitsuaki  Oita University, Faculty of Engineering, Professor, 工学部, 教授 (70026574)
MISONO Haruo  Kochi University, Faculty of Agriculture, Professor, 農学部, 教授 (30027073)
MIURA Retsu  Kumamoto University, Facukty of Medicine, Professor, 医学部, 教授 (70093466)
IMANAKA Tadayuki  Kyoto University, Faculty of Engineering, Professor, 工学部, 教授 (30029219)
ICHIYAMA Arata  Hamamatsu University School of Medicine, Professor, 医学部, 教授 (90025601)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥18,900,000 (Direct Cost: ¥18,900,000)
Fiscal Year 1997: ¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 1996: ¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1995: ¥6,900,000 (Direct Cost: ¥6,900,000)
KeywordsD-amino acid / alanine racemase / D-aspartate / D-glutamate / D-amino acid oxidase / D-serine / amino acid dehydrogenase / アルカリD-ペプチダーゼ / ヒダントイナーゼ / D-アミノアシラーゼ / D-アミノ酸代謝 / 5-置換ヒダントイン類 / バイオサーファクタント / D-アミノ酸脱水素酵素 / D-アミノペプチダーゼ
Research Abstract

We aimed to clarify de novo synthesis of D-amino acids in higher plants and animals, distribution of D-amino acids in various tissues and organs, relationship between D-amino acids and peroxisome metabolism, dynamics of D-amino acids in D-amino acid oxidase-deficient mice, change in activities if D-amino acid synthesizing enzymes, relationship between the activities of these enzymes and distribution of D-amino acids, significance of D-amino acid components in lipopetides containing D-amino acids, mechanism of molecular recognition of D-amino acids by the enzymes participating in D-amino acid metabolism, methods of specific analysis of D-amino acids with the enzymes, mechanism of D-amino acid oxidase and D-aspartate oxidase. We obtained the following results. We have clarified the function of alanine racemase occurring in mitochondria of pea seedlings. Distribution of D-aspartate and D-glutamate in various tissues of mammals and birds was also clarified. The function of enzymes participating in the biosynthesis of D-aspartate in peptides was also studied. We demonstrated a route of biosynthesis of oxalic acid through thiazolidine-2-carboxylic acid and thiazolidine-2,4-dicarboxylic acid in rats. Mechanisms of formation of D-amino acid components of peptides were also clarified. We also demonstrated biochemical mechanisms of urinary secretion of D-serine by D-amino acid oxidase-deficient mice. We designed and synthesized new peptides acting as biosurfactants based on structure-function relationship of arthrofactin. We studied enzymological properties and application of enzymes catalyzing dehydrogenation of D-threonine and D-phenylserine, hydrolysis of 5-substituted hydantoins and N-acyl D-amino acids. The tertiary structure of D-amino acid oxidase was clarified.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] C.L.Soong et al.: "Distribution of Cyclic Imide-transforming Activity in Microorganisms" FEMS Microbiol.Lett.158. 51-55 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.Ogawa et al.: "Diversity and Versatility of Microbial Hydantoin-transforming Enzymes" Eur.J.Biochem.243. 322-327 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Wakayama et al.: "Overproduction of D-Aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 in Escherichia coli and its Purification" Protein Expression and Purification. 7. 395-399 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Oda et al.: "Induction by Perooxisome Proliferators and Triiodothyronine of Serine: Pyruvate/Alanine: Glyoxylate Aminotransferase of Rat Liver" FEBS Lett. 418. 265-268 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] C.Setoyama et al.: "Structural and Functional Characterization of the Human Brain D-Aspartate Oxidase" J.Biochem.121. 798-803 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] I.Hazekawa: "A Raman Study on the C(4)=O Stretching Mode of Flavins in Flavocoenzymes: Hydrogen Bonding at the C(4)=O Moiety" J.Biochem.121. 1147-1154 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] C.L.Soong et al.: "Distribution of Cyclic Imide-transf orming Activity in Microorganisms" FEMS Microbiol.Lett. 158. 51-55 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.Ogawa et al.: "Diversity and Versatility of Microbial Hydantoin-transforming Enzymes" Eur.J.Biochem.243. 322-327 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Wakayama et al.: "Overproduction of D-Aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 in Escherichia coli and its Purification" Protein Expression and Purification. 7. 395-399 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Oda et al.: "Induction by Perooxisome Proliferators and Triiodothyronine of Serine : Pyruvate/Alanine : Glyoxylate Aminotransferase of Rat Liver" FEBS Lett. 418. 265-268 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] C.Setoyama et al.: "Structural and Functional Characterization of the Human Brain D-Aspartate Oxidase" J.Biochem.121. 798-803 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] I.Hazekawa: "A Raman Study on the C (4) =O Stretching Mode of Flavins in Flavocoenzymes : Hydrogen Bonding at the C (4) =O Moiety" J.Biochem.121. 1147-1154 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] C.L.Soong et al.: "Distribution of Cyclic Imide-transforming Activity in Microorganisms" FEMS Microbiol.Lett.158. 51-55 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] J.Ogawa et al.: "Diversity and Versatility of Microbial Hydantoin-transforming Enzymes" Eur.J.Biochem.243. 322-327 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Wakayama et al.: "Overproduction of D-Aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 in Escherichia coli and its Purification" Protein Expression and Purification. 7. 395-399 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Oda et al.: "Induction by Perooxisome Proliferators and Triiodothyronine of Serine:Pyruvate/Alanine:Glyoxylate Aminotransferase of Rat Liver" FEBS Lett. 418. 265-268 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] C.Setoyama et al.: "Structural and Functional Characterization of the Human Brain D-Aspartate Oxidase" J.Biochem.121. 798-803 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] I.Hazekawa: "A Raman Study on the C(4)=O Stretching Mode of Flavins in Flavocoenzymes:Hydrogen Bonding at the C(4)=O Moiety" J.Biochem.121. 1147-1154 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Watanabe: "Purification and kinetic properties of a D-amino-acid peptide hydrolizing enzyme from pig kidney cortex and its tentative identification with renal membrane dipeptidase" Biochim.Biophys.Acta. 1298・1. 109-118 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] R.Konno: "D-Amino-acid oxidase is not present in the mouse liver" Biochim.Biophys.Acta. (印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Mizutani: "Three-dimensional structure of porcine kidney D-amino acid oxidase at 3.0Å resolution" J.Biochem. 120・1. 14-17 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Asano: "An alkaline D-stereospecific endopeptidase with β-lactamase activity from Bacillus cereus" J.Biol.Chem.271・47. 30256-30262 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] J.Ogawa: "Imidase,a dihydropyrimidinase-like enzyme involved in the metabolism of cyclic imides" Eur.I.Biochem.(印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Wakayama: "Overproduction of D-Aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 in Escherichia coli and Its Purification" Protein Expression and Purification. 7. 395-399 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Imanaka: "Molecular Biology of Pseudomonads" ASM Press Washington,DC,USA, 9 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Suzuki: "Advances in Experimental Medicine and Biology" Plenum Publishing Co.,New York & London, 4 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M. Morikawa et al.: "A New Lipopeptide Biosurfactant Produced by arthrobacter sp. Strain MIS38" J. Bacteriol.175. 6459-6466 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] M. Wakayama et al.: "Cloning Expression, and Nucleotide Sequence of the N-Acyl-D-Aspartate Amidohydrolase Gene from Alcaligenes xylosoxydans Subsp. xylosoxydans A-6" J. Ferment. Bioeng.80. 311-317 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y. Kera et al.: "Presence of Free D-Glutamate and D-Aspartate in Rat Tissues" Biochim. Biophys. Acta. 1243. 282-286 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y. Asano et al.: "Mutants of D-Aminopeptidase with Increased Thermal Stability" J. Ferment. Bioeng.79. 614-616 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] J. Ogawa et al.: "Purification and Characterization of an ATP-Dependent Amidohydrolase, N-Methylhydantoin Amidohydrolase, from Pseudomonas putida 77" Eur. J. Biochem.229. 284-290 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] J. Biochem.: "Structures of Charge-Transfer Complexes of Flavoenzyme D-Amino Acid Oxidase : A Study by Resonance Raman Spectroscopy and Extended Huckel Molecular Orbital Method" Y. Nishina et al.118. 614-620 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K. Soda et al.: "Biochemistry of Vitamin B6 and PQQ" Stereospecificity of Aminotransferases for C-4' Hydrogen Transfer and Enzyme Evolution., (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] T. Suzuki et al.: "Intracellular Protein Catabolism" Energy-dependent Degradation of a Mutant Serine : Pyruvate/alamine : Glyoxylate Aminotransferase in a Primary Hyperoxaluria Type 1 Case, (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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