| Project/Area Number |
07308051
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 総合 |
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| Research Field |
Developmental biology
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
KATAGIRI Chiaki GRADUATE SCHOOL OF SCIENCE,HOKKAIDO UNIVERSITY,PROFESSOR, 大学院・理学研究科, 教授 (90000827)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGAMI Kenjiro LIFE SCI.INST.SOPHIA UNIV., PROFESSOR, 理工学部, 教授 (50011474)
NOMURA Kohji TOKYO METROPOL.INST.GERONT,INVESTIGATOR, 主任研究員 (30073026)
NAKANO Minoru FAC.SCI., CHIBA UNIV,, PROFESSOR, 理学部, 教授 (80009604)
KUBO Hideo TOKYO METROPOL.INST.MED.SCI., INVESTIGATOR, 研究員 (50178034)
HOSHI Motonori FAC,BIOSCI,TOKYO INST.TECHNOL., PROFESSOR, 生命理工学部, 教授 (20012411)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥9,700,000 (Direct Cost: ¥9,700,000)
Fiscal Year 1996: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1995: ¥5,500,000 (Direct Cost: ¥5,500,000)
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| Keywords | VITELLINE ENVELOPE / SPERM-VE BINDING / CHORIION / ZONA PELUCIDA / SPERM RECEPTOR / VITELLINE COAT LYSIN / OVOPEROXIDASE / GLYCOPROTEIN / オビダクチン |
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| Research Abstract |
(a) The carbohydrate moieties in 36-39KDa glycoproteins of Bufo japonicus vitelline coat are involved in binding with fertilizing sperm ; (b) cDNA encoding the major glycoprotein gp41 of Xenopus laevis vitelline envelope indicated that it is a homologue of mammalian ZP3 ; (c) The sperm receptor activity of porcine PZP3 resides in beta-Gal residues in beta-Gal residues of N-linked glycoside chains consisting fo tri-, and tetra-antennary, complex-type neutral chain ; (d) Biotinylated sea urchin sperm surface proteins were co-incubated with fucose sulfate glycoconjugates isolated from the jelly coat and were chromatographically separated, with the result that only two sperm proteins were coeluted with the pertinent jelly coat molecules ; (e) Employing the soft fertilization envelope of sea urchin eggs, a 70kDa ovoperoxidase (OPO) was isolated, and its primary structure elucidated on the basis of cloned cDNA indicated the substrate requirement of hatching enzyme ; (f) Comparison of the cDNA-based primary structures of the vitelline coat lysins from 7 species of Archeogastropods revealed that the species-specific sequences reside in 40-100 amino acid residues in the central portion of each molecule ; (g) The jelly coat of the starfish eggs was differentially irradiated by high-energy electrons and the acrosome-reaction-inducing (ARI) activity was monitored, with the result that the minimum size exhibiting ARI activity was of 14kDa molecules containing a plenty amount of sugar residues ; (h) Cloning of cDNAs for the egg envelope proteins of Oryzias latipes revealed that a low molecular-weight subunit (ZI-3) is homologous with mammalian ZP3, and its gene is expressed exclusively in the liver but not in the ovary.
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