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Physiological studies on the mechanisms of synaptic transmission.

Research Project

Project/Area Number 07308054
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section総合
Research Field 神経・脳内生理学
Research InstitutionNagoya University (1996)
佐賀医科大学 (1995)

Principal Investigator

KUBA Kenji  Nagoya University, School of Medicine, Professor, 医学部, 教授 (60080561)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Tomoyuki  Tokyo University, School of Medicine, Professor, 医学部, 教授 (40092415)
HIGASHI Hideho  Kurume University, School of Medicine, Professor, 医学部, 教授 (10098907)
OZAWA Seiji  Gunma University, School of Medicine, Professor, 医学部, 教授 (40049044)
TAKASI Akasu  Kurume University, School of Medicine, Professor, 医学部, 教授 (60113213)
AKAIKE Norio  Kyushu University, School of Medicine, Professor, 医学部, 教授 (30040182)
平野 丈夫  京都大学, 医学部, 教授 (50181178)
木島 博正  名古屋大学, 理学部, 教授 (30012397)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥16,800,000 (Direct Cost: ¥16,800,000)
Fiscal Year 1996: ¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 1995: ¥8,800,000 (Direct Cost: ¥8,800,000)
KeywordsSynapses / Neurotransmitters / Intracellular Ca^<2+> / Plasticity / Glutamate receptors / Muscarinic receptors / Presynaptic terminals / Postnatal development
Research Abstract

This research project was organized to promote the understanding of the mechanism of synaptic transmission and composed of 14 physiologists studying synaptic transmission mechanism. New findings were presented and extensively discussed in the meetings held once a year term, leading to the new aspect of understanding of the mechanisms of synaptic transmission. The principal new findings obtained are as follows.
(1) The types of Ca^<2+> channels involved in the exocytosis of neurotransmitters in various types of neurons were identified. The evidence for the role of high Ca^<2+> concentration in Ca^<2+> channel domain in exocytosis was shown. Such a high Ca^<2+> was removed by not only Na/Ca exchange and Ca pumps, but also a new unknown fast process.
(2) The subunit structures of glutamate ionotropic receptors, their distributions and contributions to the generation of postsynaptic potentials and long-term synaptic plasticities, and their developmental changes were clarifiled in hippocampal and cerebellar neurons.
(3) The mechanisms of interactions between G-proteins and metabotropic receptors and between G-proteins and kinases and ion channels and the involvement of these interactions in presynaptic enhancement and inhibitions were demonstrated in various types of neurons.
(4) The evidence for some roles of glial cells in synaptic transmission was presented. A new gene involved in the development of synapses was cloned.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (57 results)

All Other

All Publications (57 results)

  • [Publications] 久場 健司: "細胞内Ca^<2+>の調節機構" Clinical Neuroscience. 14. 138-142 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 久場 健司: "記憶の神経回路-シナプスでの細胞内Ca^<2+>調節とその役割-" Clinical Calcium. 5. 9-13 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshizaki,K.: "Ca^<2+>-induced Ca^<2+> release and its activation in response to a single action potential in rabbit otic ganglion cells." J. Physiology. 486. 177-187 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ebihara,S.: "Gramicidin-perforated patch recording : GABA response in mammalian neurones with intact intracellular chloride." J.Physiology. 484. 77-86 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suzuki,T.: "MAO inhibitors clorgyline and lazabemide, prevent hydroxyl radical generation caused by brain ischemia/reperfusion in mice." Pharmacology. 50. 357-362 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamamoto,T.: "Caffeine rapidly decreases potassium conductance of dissociated outer hair cells of guinea-pig cochlea." Brain Research. 677. 89-96 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shimizu.T.: "Role of A S afferent fibers in modulation of primary afferent input to the adult rat spinal cord." Brain Research. 691. 92-98 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 東 英穂: "脳虚血とイオンチャネル" 日本蘇生学会雑誌. 13. 100-103 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 東 英穂: "実験的脳虚血による海馬錐体細胞の不可逆的変化発生機序" Brain Hypoxia. 9. 131-154 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Silver R.A.: "Non-NMDA receptor occupancy during transmission at rat cerebellar mossy fibre-granule cell synapses with only one functional release site" Journal of Physiology (London). 489. 169 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Larkman P.M: "Adenosine 3':5'-cyclic monophosphate mediates a 5HF induced response in neonatal rat motoneurons." Pfliigers Arch. 430. 763-769 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takei,Y.: "Synapsin I deficiency results in the structural change in the presynatric teminals in the murine nervous system." Journal of cell Biology. 131. 1789-1800 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akasu,T.: "Synaptic transmission and function of parasympathetic ganglia." Progress in Neurobiology. 45. 459-522 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tokimasa,T.: "Inhibition by wortmannin of M-current in bullfrog sympathetic neurones." British Journal of Pharmacology. 114. 489-495 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hasuo,H.: "Muscarine increases a voltage-independent potassium conductance through an M4 receptor in rat dorsolateral septal nucleus neurons." Neuroscience Letters.189. 163-166 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Isa,T.: "Spermine mediates inward rectification of Ca^<2+>-permeable AMPA receptor channels." NeuroReport. 6. 2045-2048 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Isa,T.: "Distribution of neurones expressing inwardly rectifying and Ca^<2+>-permeable AMPA receptors in rat hippocampal slices." J. Physiol. (Lond). 491. 719-733 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Isa,T.: "Spermine blocks synaptic transmission mediated by Ca^<2+>-permeable AMPA receptors." NeuroReport. 7 (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 久場 健司: "シナプス伝達のダイナミクス" 培風館, 146 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshizaki, K., Hoshino, T., Sato, M., Koyano, H., Nohmi, M., Hua, S.-Y.& Kuba, K.: "Ca^<2+>-induced Ca^<2+> release and its activation in response to a single action ganglion cells." J.Physiol.Lond.486. 177-187 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Komatsu, H., Mori, I., Rhee, J.S., Akaike, N.& Oshima, Y.: "Mutations in a cyclic nucleoride-gated channel lead to abnormal thermosensation and chemosensation in C,elegans." Neuron. 17. 707-718 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Xu, T.L., Nabekura, J.& Akaike, N.: "Protein kinase C-mediated enhancement of glycine response in rat sacral dorsal commissural neurones by serotonin." J.Physiol.Lond.496. 491-501 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hasuo H,Akasu T & Gallagher JP: "Muscarine activates a nonselectivecation current through a M3 muscarinic receptor subtype in rat dorsolateral septal nucleus neurons." J Neurophysiol. 76. 2221-2230 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamada K & Akasu T: "Substance P suppresses GABAA receptor function via protein kinase C in primary sensory neurones of bullfrogs." J Physiol.496. 439-449 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Isa T,Iino M & Ozawa S: "Spermine blocks synaptic transmission mediated by Ca^<2+>-permeable AMPA receptors." NeuroReport. 7. 689-692 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Iino M,Koike M,Isa T & Ozawa S: "Voltage-dependent blockage of Ca^<2+>-permeable AMPA receptors by joro spider toxin in cultured rat hippocampal neurones." J.Physiol.(Lond.). 496. 431-437 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fujimura N,Tanaka E,Yamamoto S,Shigemori M,& Higashi H: "Contribution of ATP-sensitive potassium channels to hypoxic hyperpolarization in rat hippocampal CA1 neurons in vitro." J.Neurophysiol.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamamoto S,Tanaka E,& Higashi H: "Mediation by intracellular calciumdependent signals of hypoxic hyperpolarization in rat hippocampal CA1 neurons in vitro." J.Neurophysiol.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hori Y,Endo K & Takahashi T: "Long-lasting synaptic facilitation induced by serotonin in superficial dorsal hornneurones of rat spinal cord." Journal of Physiology (London). 492. 867-876 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sliver RA,Cull-Candy SG & Takahashi T: "Non-NMDA glutamate receptor occupancy and open probability at a rat cerebellar synapse with single with single and multiple release sites." Journal of Physiology (London). 494. 230-250 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshizaki,K: "Ca^<2+>-induced Ca^<2+> release and its activation in response to a single action potential in rabbit otic ganglion cells" J.Physiol.(Lond.). 486. 177-187 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Komatsu,H.: "Mutations in a cyclic nucleotide-gated channel lead to abnormal thermosensation and chemosensation in C.elegans." Neuron. 17. 707-718 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Iino,M.: "voltage-dependent blockage of Ca^<2+>-permeable AMPA receptors by joro spider toxin in cultured rat hippocampal neurones." J.Physiol.(Lond.). 496. 431-437 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takahashi,T.: "Presynaptic calcium current modulation by a glutamate receptor." Science. 274. 594-597 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Osanai,M.: "Effects of a trintrobenzene analogue on the transmitter release at the frog neuromuscular junction." J.Neurophysiol.76. 1735-1743 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kadotani,H.: "Motor discoordination results from combined gene disruption of the NMDA receptor NR_2A and NR2e subunits,but not from single disruption of the NR2A or NR2C subunits" J.Neurosci.16. 7859-7867 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 久場健司: "シナプス伝達のダイナミックス" 培風館, 146 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 久場健司: "新しい光学顕微鏡第2巻 第3章-5-(3) 「ニューロンのCa^<2+>動態」" 学際企画, 199 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 久場健司: "細胞内Ca^<2+>の調節機構" Clinical Neuroscience. 14. 138-142 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 久場健司: "記憶の神経回路-シナプスでの細胞内Ca^<2+>の調節とその役割-" Clinical Calcium. 5. 9-13 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoshizaki,K.: "Ca^<2+>-induced Ca^<2+>release and its activation in response to a single action potential in rabbit otic ganglion cells." J.Physiology. 486. 177-187 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ebihara,S.: "Gramicidin-perforated patch recording : GABA response in mammalian neurones with in tact intracellular chloride.," J.Physiology. 484. 77-86 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Suzuki,T.: "MAO inhibitors.clorgyline and lazabemide pre-vent hydroxyl radical generation caused by brain ischemia/reperfusion in mice." Pharmacology. 50. 357-362 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yamamoto,T.: "Caffeine rapidly decreases potassium conductance of dissociated outer hair cells of guinea-pig cochlea." Brain Research. 677. 89-96 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Akasu,T: "Synaptic transmission and function of parasympathetic ganglia." Progress in Neurobiology. 45. 459-522 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tokimasa,T.: "Inhibition by wortmannin of M-current in bullfrog Sympathetic neurones." British Journal of Pharmacology. 114. 489-495 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hasuo,H.: "Muscarine increases a voltage-independent potassium conductance through an M4 receptor in rat dorsolateral septal nucleus neurons." Neuroscience Lellers.189. 163-166 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Isa,T.: "Spermine mediates inward rectification of Ca^<2+>-permeable AMPA rece-ptor.channels." NeuroReport. 6. 2045-2048 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Isa,T.: "Distribution of neurones expressing inwardly rectifying and Ca^<2+>-permeable AMPA receptors in rat hippocampal slices." J.Physiol. (Lond.). 491. 719-733 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Isa,T.: "Spermine blocks synaptic transmission mediated by Ca^<2+>-permeable AMPA receptors." NeuroReport. 7 (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shimizu,T.: "Role of A δ afferent fibers in modulation of primary offerent input to the adult rat spinal cord." Brain Research. 691. 92-98 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 東 英穂: "脳虚血とイオンチャネル" 日本蘇生学会雑誌. 13. 100-103 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 東 英穂: "実験的脳虚血による海馬錐体細胞の不可逆的変化発生機序" Brain Hypoxia. 9. 131-154 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Silver R.A.: "Non-NMDA receptor occupancy during transmission at rat cerebellar mossy fibre-granule cell synapses with only one functional release site" Journal of Physiology (London). 489. 16 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Larkman P.M.: "Adenosine 3′ : 5′-cyclic monophosphate mediates a 5HT-induced response in neonatal rat motoneurons." Pflugers Arch. 430. 763-769 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takei,Y.: "Synapsin I deficiency results in the structural change in the Presynaptic terminals in the murine nervous system." Journal of Cell Biology. 131. 1789-1800 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 久場健司: "シナプス伝達のダイナミクス" 培風館, 146 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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