| Project/Area Number |
07308069
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 総合 |
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| Research Field |
Structural biochemistry
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| Research Institution | University of Tokyo |
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Principal Investigator |
TOKUDA Hajime University of Tokyo, Institute of Molecular and Cellular Biosciences, Professor, 分子細胞生物学研究所, 教授 (40125943)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Makoto Oita Medical University, Department of Biochemistry, Professor, 医学部, 教授 (20142408)
YAMANE Kunio University of Tsukuba, Institute of Biological Sciences, Professor, 生物科学系, 教授 (20013336)
HIRATA Hajime Institute of Technology, Faculty of Science, Professor, 理学部, 教授 (40049052)
ENDO Toshiya Nagoya University, Faculty of Science, Professor, 理学部, 教授 (70152014)
ITO Koreaki Kyoto University, Institute of Virus Research, Professor, ウィルス研究所, 教授 (90027334)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥9,600,000 (Direct Cost: ¥9,600,000)
Fiscal Year 1996: ¥9,600,000 (Direct Cost: ¥9,600,000)
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| Keywords | Protein translocation / Prokaryote / Reconstitution / SecY / SecE / SecG / SecA |
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| Research Abstract |
1. SecG was found to interact with SecA and facilitate the membrane insertion-deinsertion cycle of SecA.
2. Mode of interaction of SecY-SecE-SecG complex was revealed.
3. SecA was found to be involved in the import of cytochrome f into thylakoid.
4. SecY and SecE were reconstituted into a planar lipid bilayr membrane and found to form a ion-conducting channel.
5. Step-wise translocation of precursor protein was found to be caused by a hydrophobic region of the precursor.
6. Structure and function of Bacillus subtilis SRP homologue were revealed.
7. Highly active protein translocation system was reconstituted into proteoliposomes.
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