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Molecular mechanism of force generation in muscle fibers

Research Project

Project/Area Number 07308075
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section総合
Research Field 神経・脳内生理学
Research InstitutionOita Medical University

Principal Investigator

YAMADA Kazuhiro  Oita Med.Univ., Prof., 医学部, 教授 (20053027)

Co-Investigator(Kenkyū-buntansha) KODAMA Takao  Kyushu Institute of Technology, Prof., 情報工学部, 教授 (30034200)
YANAGIDA Toshio  Osaka Univ.Prof., 基礎工学部, 教授 (30089883)
IINO Masamitsu  Faculty of Medicine, Univ.Tokyo.Prof., 医学部, 教授 (50133939)
HOTTA Yoshiki  Univ.Tokyo.Prof., 大学院・理学研究科, 教授 (30010036)
SUGI Haruo  Teikyo Univ., Sch.Med., Prof., 医学部, 教授 (20082076)
八木 直人  東北大学, 医学部, 講師 (80133940)
平塚 寿章  旭川医科大学, 医学部, 助教授 (30041825)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1996: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordscaged ATP / muscle fibers / fluorescent ATP analogue / myosin head / electron microscopy / crossbridges / X-ray diffraction / ryanodine receptors / 筋肉 / 生体運動 / 蛍光プローブ / 構造変化 / 運動単分子
Research Abstract

Since the introduction of caged ATP and caged calcium demembranated skinned muscle fibers have become more important preparation for elucidating the molecular mechanism of muscle contraction. Yamada studied a novel fluorescence signal from muscle fibers labeled by using a fluorescent nucleotide analog. Sugi studied the efficiency of chemo-mechanical energy conversion and showed how the efficiency was determined due to the load against which muscle should work. Hiratsuka developed a new fluorescent ATP analogue useful for studying the chemo-mechanical energy conversion. Arata spin-labeled the base part of the myosin head in muscle fibers and showed that they were randomly oriented even during contraction. Hotta produced several useful mutants of Drosophila flight muscles in which actin molecules were mutated. T.Wakabayashi used cryo electron microscopy and Katayama used quick-freeze deep-etch replica images by electron microscopy to directly observe force-generating crossbridges. Ishiwata studied the mechanism of sliding of single motor molecules by using optical tweezers. K.Wakabayashi, Yagi and Umazume studied time-resolved X-ray diffraction of contracting muscle fibers. Yanagida was able to directly observe single myosin molecules to bind and cleave ATP molecules and to produce force. Iino and Kurebayashi studied subtypes of ryanodine receptors in relation to the excitation-contraction coupling. Ohtsuki studied the role playd by troponin T associated with the mechanism of calcium regulation of the contractile system. Tawada and Kodama worked on theoretical as well as thermodynamical approach to the problem.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (106 results)

All Other

All Publications (106 results)

  • [Publications] Emoto,Y: "Tension relaxation induced by pulse photolysis of caged ATP in partially crosslinked fibers from rabbit psoas muscle." Proc.Natl Acad.Sci.USA. 92. 1461-1464 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kagawa,K: "BDM compared with Pi and low Ca^<2+> in the cross-bridge reaction initiated by flashphotolysis of caged ATP." Biophysical Joutnal. 68. 2590-2600 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiratsuka,T: "Monitoring the Myoshin ATPase Reaction Using a Sensitive Fluorescent Probe Pyrene-Labeled ATP" Biophys.J.(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 平塚寿章: "解説 蛍光標識ヌクレオチド-蛋白質の構造と機能の研究への活用-" 蛋白質・核酸・酵素. 142. 64-72 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yagi,N: "Small-angle X-ray Diffraction of Muscle Using Undulator Radiation from the Tristan Main Ring at KEK" Synchrotron Rad.3. 305-312 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Honda,H.: "Cross-bridge movement in rat slow skeletal muscle as a function of calcium concentration" Pflugers Arch.432. 797-802 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wada,H.: "Effect of inorganic phosphate and ADP on the myofilament sliding induced by laser flash photolysis of caged ATP." Biochim.Biophys.Acta. 1274. 89-93 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sugiura,S.: "Different cardiac myosin isofroms exhibit equal force-generating ability in vitro." Biochim.Biophys.Acta. 1275. 73-76 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] An,H.: "Isolation of 88F actin mutants of Drosophila melanogaster and possible alterations in the mutant actin structures." J.Mol.Biol.260. 492-505 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akiyama,Y.: "The gcm-motif,A nove DNA binding motif conserved in Drosophila and mammals" Proc.Natl.Acad.Sci.USA. 93. 14912-14916 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamamoto,K: "Myosin from alga Chara : Unique structure revealed by electron microscopy" J.Mol.Biol.254. 109-112 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Katayama,E.: "Mode of caldesmon binding to smooth muscle thin flilament : Possible projection of the amino-terminal domain of caldesmon from native thin filament." Biophys.J.68. 2419-2428 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Morimoto,S.: "Reduced positive freeback regulation between myosin crossbridge and cardiac troponin C in fast skeletal myofibrils." J.Biochem.119. 737-742 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kawashima,A.: "Troponin isoform depencent pH dependence of the Ca^<2+>-activated myofibrillar ATPase activity of avian slow and fast skeletal muscles." Biochem.Biophys.Res.Commun.207. 585-592 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamazawa,T.: "A region of the ryanodine receptor critical for excitation-contraction coupling in skeletal muscle." J.Biol.Chem.(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamazawa,T.: "Subtype specificity of ryanodine receptor for Ca^<2+> signal amplification in excitation-contraction coupling" EMBO J.15. 6172-6177 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Emoto,Y.: "Force production by chemically crosslinked myosin-actin cross-bridges in rabbit skinned fibers in response to MgATP depletion." Biophys.Chem.61. 85-92 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Imafuku,Y.: "Fluctuation in the microtubule sliding movement driven by kinesin in vitro." Biophys.J.70. 878-886 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Arata,T.: "A myosin head can interact with two chemically modified G-actin monomers at ATP-modulated multiple sites." Biochemistry. 35. 16061-16068 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Murai,S.: "Binding of myosin and its subfragment-1 with antibodies specific to the two heads of the myosin mulecule." J.Biochem.117. 974-979 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kurebayashi,N.: "Stimulating effect of polyols on Ca^<2+> release channel/ryanodine receptors of frog skeletal muscle." J.Muscle Res.Cell Motil.17. 283 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Temma,K.: "Doxorubicin alters Ca^<2+> transient but fails to change Ca^<2+> sensitivity of contractile proteins" Environment.Toxicol.Pharmacol.1. 131-139 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suzuki,M.i: "Coupling of protein surface hydrophobicity cahnge to ATP hydrolysis by myosin motor domain." Biophys.J.72. 18-23 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suzuki,M.: "Hydration of proteins studied by microwave dielectric analysis" J.Phys.chem.100. 7279-7282 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Iwamoto,H.: "Effect of stretch and release on equatorial X-ray diffraction during a twitch contraction of frog skeletal muscle." Biophys.J.68. 227-234 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fujita,H.: "Structural and functional reconstitution of thin filaments in the contractile apparatus of cardiac muscle." Biophys.J.71. 2307-2318 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nishizawa,T.: "Unbinding force of a single motor molecule of muscle measured using optical tweezers." Nature. 377. 251-254 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Emoto, Y., Tawada, K & Yamada, K.: "Tension relaxation induced by pulse photolysis of caged ATP in partially crosslinked fibers from rabbit psoas muscle." Proc.Natl Acad.Sci.USA. 92. 1461-1464 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kagawa, K., Horiuti, K.& Yamada, K.: "BDM compared with Pi and low Ca^<2+> in the crossbridge reaction initiated by flash photolysis of caged ATP." Biophysical Journal. 68. 2590-2600 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiratsuka, T.: "Monitoring the myoshin ATP ase reaction using a sensitive fluorescent probe pyrene-labeled ATP." Biophysical Journal. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yagi, N.: "Small-angle X-ray diffraction of muscle using undulator radiation from the tristan main ring at KEK" Syncrotron Red.3. 305-312 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Honda H.: "Cross-bridge movement in rat slow skeletal muscle as a function of calsium concentration." Pflugers Arch.432. 797-802 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wada, H.: "Effect of inorganic phosphate and ADP on the myofilament sliding induede by lser flash photolysis of caged ATP" Biochim.Biophys.Acta.59-93 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sugiura, S.: "Different cardiac myosin isoforms exhibit equal force-generating ability in vitro." Biochim.Biophys.Acta.1275. 73-76 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] An, H.: "Isolation of 88F actin mutants of Drosophila melanogaster and possible alterations in the mutant actin structures." J.Mol.Biol.260. 492-505 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akiyama, Y.: "The gcm-motif.A nove DNA binding motif conserved in Drosophila and mammals." Proc.Natl.Acad.Sci.USA. 93. 14912-14916 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamamoto, K.: "Myosin form alga Chara : Unique structure revealed by electron microscopy." J.Mol.Biol.254. 109-112 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Morimoti, S.: "Reduced positive freeback regulation between myosin crossbridge and cardiac troponin Cin fast skeletal myofibrils." J.Biochem.119. 737-742 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kawashima, A.: "Troponin isoform dependent pH dependence of the Ca^<2+>-activated myofibrillar ATPase activity of avian slow and fast skeletal muscles." Biochem.Biophys.Res.Commun.207. 585-592 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamazawa, T.: "A region of the ryanodine receptor critical for excitation-contraction coupling in skeletal muscle." J.Biol.Chem.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamazawa, T.: "Subtype specificity of ryanodine receptor for Ca^<2+> signal amplification in excitation-contraction coupling." EMBO J.15. 6172-6177 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Emoto, Y.: "Force production by chemically crosslinked myosin-actin cross-bridges in rabbit skinned fibers in response to MgATP depletion." Biophys.Chem.61. 85-92 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Imafuku, Y.: "Fluctuation in the microtubule sliding movement driven by kinesin in vitro." Biophys.J.70. 878-886 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Arata, T.: "A myosin head can interact with two chemically modified G-actin monomers at ATP-modulated multiple sites." Biochemistry. 35. 16061-16068 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Murai, S.: "Binding of myosin and its subfragment-1 with antibodies specific to the two heads of the myosin molecule." J.Biochem.117. 974-979 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kurebayashi, N.: "Stimulating effect of polyols on Ca^<2+> release channel/ryanodine receptors of frog skeletal muscle." J.Muscle Res.Cell Motil. 17. 283 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Temma, K.: "Doxorubicin alters Ca^<2+> transient but fails to change Ca^<2+> sensitivity of contractile proteins." Environment.Toxicol.Pharmacol.1. 131-139 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suzuki, M.: "coupling of protein surface hydrophobicity change to ATP hydrolysis by myosin motor domain." Biophys.J.72. 18-23 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suzuki, M.: "Hydration of proteins studied by microwave dielectric analysis." J.Phys.Chem.100. 7279-7282 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Iwamoto, H.: "Effect of stretch and release on equatorial X-ray diffraction during a twitch contraction of frog skeletal muscle." Biophys.J.68. 227-234 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fujita, H.: "Structural and functional reconstitution of thin filaments in the contractile apparatus of cardiac muscle." Biophys.J.71. 2307-2318 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nishizaka, T.: "Unbinding force of a single motor molecule of muscle measured using optical tweezers." Nature. 377. 251-254 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Emoto, Y: "Tension relaxation induced by pulse Photolysis of caged ATP in partially crosslinked fibers from rabbit psoas muscle." Proc.Natl Acad.Sci.USA. 92. 1461-1464 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kagawa,K: "BDM compared with Pi and low Ca ^<2+> in the cross-bridge reaction initiated by flashphotolysis of caged ATP." Biophysical Joutnal. 68. 2590-2600 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hiratsuka, T: "Monitoring the Myoshin ATPase Reaction Using a Sensitive Fluorescent Probe Pyrene-Labeled ATP" Biophys.J.in press. (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 平塚寿章: "解説 蛍光標識ヌクレオチド-蛋白質の構造と機能の研究への活用-" 蛋白質・核酸・酵素. 142. 64-72 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yagi, N.: "Small-angle X-ray Diffraction of Muscle Using Undulator Radiation from the Tristan Main Ring a KEK" Synchrotron Rad.3. 305-312 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Honda,H.: "Cross-bridge movement in rat slow skeletal muscle as a function of calcium concentration" Pflugers Arch.432. 797-802 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Wada, H.: "Effect of inorganic phosphate and ADP on the myofilament sliding induced by laser flash photolysis of caged ATP." Biochim.Biophys.Acta. 1274. 89-93 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sugiura,S.: "Different cardiac myosin isoforms exhibit equal force-generating ability in vitro." Biochim.Biophys.Acta. 1275. 73-76 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] An, H.: "Isolation of 88F actin mutants of Drosophila melanogaster and possible alterations in the mutant actin structures." J.Mol.Biol.260. 492-505 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Akiyama, Y.: "The gcm-motif, A nove DNA binding motif conserved in Drosophila and mammals" Proc.Natl.Acad.Sci.USA. 93. 14912-14916 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamamoto, K: "Myosin from alga Chara : Unique structure revealed by electron microscopy" J.Mol.Biol.254. 109-112 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Katayama, E.: "Mode of caldesmon binding to smooth muscle thin flilament : Possible projection of the amino-terminal domain of caldesmon from native thin ament." Biophys.J.68. 2419-2428 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Morimoto, S.: "Reduced positive freeback regulation between myosin crossbridge and cardiac troponin C in fast skeletal myofibrils." J.Biochem.119. 737-742 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kawashima, A.: "Troponin isoform depencent pH dependence of the Ca^<2+>-activated myofibrillar ATPase activity of avian slow and fast skeletal muscles." Biochem.Biophys.Res.Commun.207. 585-592 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamazawa, T.: "A region of the ryanodine receptor critical for excitation-contraction coupling in skeletal muscle." J.Biol.Chem.in press. (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamazawa, T.: "Subtype specificity of ryanodine receptor for Ca^<2+> signal amplificaiton in excitation-contraction coupling" EMBO J.15. 6172-6177 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Emoto, Y.: "Force production by chemically crosslinked myosin-actin cross-bridges in rabbit skinned fibers in response to MgATP depletion." Biophys.Chem.61. 85-92 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Imafuku, Y.: "Fluctuation in the microtubule sliding movement driven by kinesin in vitro." Biophys.J.70. 878-886 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Arata, T.: "A myosin head can interact with two chemically modified G-actin monomers at ATP-modulated multiple sites." Biochemistry. 35. 16061-16068 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Murai, S.: "Binding of myosin and its subfragment-1 with antibodies specific to the two heads of the myosin mulecule." J.Biochem.117. 974-979 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kurebayashi, N.: "Stimulating effect of polyols on Ca^<2+> release channel/ryanodine receptors of frog skeletal muscle." J.Muscle Res.Cell Motil.17. 283p- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Temma, K.: "Doxorubicin alters Ca^<2+> transient but fails to change Ca^<2+> sensitivity of contractile proteins" Environment.Toxicol.Pharmacol.1. 131-139 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Suzuki, M.i: "Coupling of protein surface hydrophobicity cahnge to ATP hydrolysis by myosin motor domain." Biophys.J.72. 18-23 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Suzuki, M.: "Hydration of proteins studied by microwave dielectric analysis" J.Phys.chem.100. 7279-7282 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Iwamoto, H.: "Effect of stretch and release on equatorial X-ray diffraction during a twitch contraction of frog skeletal muscle." Biophys.J.68. 227-234 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Fujita, H.: "Structural and functional reconstitution of thin filaments in the contractile apparatus of cardiac muscle." Biophys.J.71. 2307-2318 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nishizaka, T.: "Unbinding force of a single motor molecule of muscle measured using optical tweezers." Nature. 377. 251-254 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Emoto,Y.: "Tension relaxation induced by pulse photolysis of caged ATP in partially crosslinked fibers from rabbit psoas muscle." Proc.Natil.Acad.Sci.USA. 92. 1461-1464 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yamada,K.: "Labeling of myosin with a fluorescent analog of ATP and the fluorescent transients after photolysis of caged nucleotides in skeletal muscle fibers." Biophysical Journal. 68. 331s (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kagawa,K.: "BDM compared with Pi and low Ca2+ in the cross-bridge reaction initiated by flash photolysis of caged ATP." Biophysical Journal. 68. 2590-2600 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 平塚寿章: "[総説]ミオシンのATP結合ポケットの構造変化と筋収縮" 生物物理. 35. 2590-2600 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hiratsuka,T.: "Nucleotide-induced closure of the ATP-binding pocket in myosin subfragment-1" Journal of Biological Chemistry. 269. 27251-27257 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yagi,N.: "Structural changes in myosin cross-bridges during shortening of frog skeletal muscle." Journal of Muscle Research and Cell Motility. 16. 57-63 (1995)

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      1995 Annual Research Report
  • [Publications] Hosoya,T.: "glial cells missing : A binary switch between neural and glial determination in Drosophila." Cell. 82. 1025-1036 (1995)

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      1995 Annual Research Report
  • [Publications] Chiba,A.: "Fasciclin III as a synaptic target recognition molecule in Drosophila" Nature. 374. 166-168 (1995)

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      1995 Annual Research Report
  • [Publications] Goto,S.: "Enhancer trapping of positional information corresponding to the polar coodinate system in the imaginal disc of Drosophila melanogaster." Roux's Archiv Dev.Biol.204. 378-391 (1995)

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      1995 Annual Research Report
  • [Publications] Ikemoto,T.: "Enhancing effect of calmodulin on Ca2+-induced Ca2+ release in the sarcoplasmic reticulum of rabbit skeletal muscle fibres" Journal of Physiology. 487. 573-582 (1995)

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      1995 Annual Research Report
  • [Publications] Takeshima,H.: "Ca2+ omdiced Ca2+ release in myocytes from dyspedic mice lacking type-1 ryanodine receptor." EMBO J.14. 1025-1036 (1995)

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      1995 Annual Research Report
  • [Publications] Yamamoto,K.: "Actin based motor protein from fresh water alga Chara corallina" Journal of Molecular Biology. (in press). (1995)

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      1995 Annual Research Report
  • [Publications] Katayama,E.: "Effect of caldesmon on the assamby of smooth muscle myosin." Journal of Biological Chemistry. 270(8). 3319-3925 (1995)

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      1995 Annual Research Report
  • [Publications] Katayama,E.: "Mode of caldesmon binding to smooth musle thin filament : Possible projection of the amino-terminal domain of caldesmon from native thin filament." Biophysical Journal. 68(6). 2419-2428 (1995)

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  • [Publications] Iwamoto,H.: "Effect of stretch and release on equatorial X-ray diffraction during a twitch contraction of frog skeletal muscle." Biophysical Journal. 68. 227-234 (1995)

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      1995 Annual Research Report
  • [Publications] Sugimoto,Y.: "Conformational changes of the myosin heads during hydrolysis of ATP as analyzed by X-ray solution scattering." Biophysical Journal. 68. 29s-34s (1995)

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      1995 Annual Research Report
  • [Publications] Yagi,N.: "A real-time observation of X-ray diffraction from frog skeletal muscle during and after slow length changes." Japan Journal of Physiology. 45. 583-606 (1995)

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      1995 Annual Research Report
  • [Publications] Kawashima,A.: "Troponin isoform dependent pH dependence of Ca2+-activated myofibrillar ATpase activity of avian slow fast skeletal muscles." Biochem.Biophys.Res.Commun.207. 585-592 (1995)

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  • [Publications] Yanagida,T.: "Force and steps generated by single myosin molecues." Biophys.J.68. 312-320 (1995)

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      1995 Annual Research Report
  • [Publications] Funatsu,T.: "Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution." Nature. 374. 555-559 (1995)

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      1995 Annual Research Report
  • [Publications] Higuchi,H.: "Gompliance of thin filaments in skinned fibers of rabbit skeletal muscle." Biophys.J.69. 1000-1010 (1995)

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  • [Publications] Ishijima,A: "Multiple-and single-molecule analysis of the actomyosin motor using nanometerpiconewton manipulation with a microneedle : Unitary step and force." Biophys.J.70. 383-400 (1996)

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  • [Publications] Vate,R.V.: "Direct observation of single kinesin molecules moving along microtubules." Nature. (in press). (1996)

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      1995 Annual Research Report
  • [Publications] Sugiura,S.: "Force-velocity relations of rat cardiac myosin isozymes sliding on algal cell actin cables in vitro." BBA (Biochemica et Biophysica Acta). 1231. 69-75 (1995)

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      1995 Annual Research Report
  • [Publications] Kobayashi,T.: "Effect of lateral forces on the movement of myosin-coated beads on actin cables studied using a centrifuge microscope." Japanese Journal of Physiology. 45. 177-186 (1995)

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      1995 Annual Research Report
  • [Publications] Chaen,S.: "The force-velocity relationship of the ATP-dependent actin-myosin sliding causing cytoplasmic streaming in algal cells,studied using a centrifuge microscope." The Journal of Experimental Biology. 198. 1021-1027 (1995)

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  • [Publications] Shirakawa,I.: "The mode of ATP-dependent microtubule-kinesin sliding in the auxotonic condition." The Journal of Experimental Biology. 198. 1809-1815 (1995)

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      1995 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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