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Structure and Function of Novel Neuron-specific Calcium-binding Proteins

Research Project

Project/Area Number 07309013
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section総合
Research Field 広領域
Research InstitutionGraduate School of Science, Osaka University

Principal Investigator

KAWAMURA Satoru  Graduate School of Science, Osaka University, Professor, 大学院・理学研究科, 教授 (80138122)

Co-Investigator(Kenkyū-buntansha) HIDAKA Hiroyoshi  Medical School, Nagoya University, Professor, 医学部, 教授 (80100171)
MIKI Naomasa  Medical School, Osaka University, Professor, 大学院・医学部, 教授 (40094445)
TOKUNAGA Fumio  Graduate School of Science, Osaka University, professor, 大学院・理学研究科, 教授 (80025452)
TAKAMATSU Ken  Medical School, Toho University, Professor, 医学部, 教授 (90154898)
KUNO Takayoshi  Medical School, Kobe University, Professor, 医学部, 教授 (50144564)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 1996: ¥7,800,000 (Direct Cost: ¥7,800,000)
Fiscal Year 1995: ¥7,000,000 (Direct Cost: ¥7,000,000)
Keywordscalcium-binding proteins / Neuron / S-modulin / Hippocalcin / recoverin / neurocalcin / NVP / NVP / S-モジュリン / カルモジュリン / リン酸化 / 神経
Research Abstract

(1) Molecular cloning of new family members of neuron-specific calcium-binding proteins (NCaP) were performed : we obtained clones of frog rod photoreceptor S-modulin ; frog cone photoreceptor s26 ; rat hippocampal HLP3 and HLP4.
(2) Expression systems in E.Coli are now available in some of the NCaPs. They included Hippocalcim, S-modulin and s26.
(3) Hippocalcin genome gene was isolated and it was found that this gene is located on the 2nd human chromosome. The transcription mechansism of rat hippocalcin gene was investigated and the sites of enhancer and silencer were identified.
(3) The target proteins of S-modulin and neurocalcin were found. In the case of S-modulin, rhodopsin kinase was found to be the target protein. This was done by functional analysis and also structural analysis using a liker conjugated with S-modulin. The possible target of neurocalcin was found to be S100b and/or tublin b.
(4) The N-terminus of recoverin is acylated with myristic acid. The role of this lipid modification was examined. It was found that myristoylation facilitates the binding of recoverin to rhodopsin kinase. It was also demonstrated that the lipid modification induces facilitation of the targetting of rhodopsin kinase to disk membranes. The membrane binding is weaker in s26 than S-modulin. It was found that part of this difference is due to a lack of positive charges in s26 at amino acid residues close to C-terminus.
(5) Possible role of calmodulin on the phosphorylation of muscarinic acetylcholine receptor was investigated. The calcium-bound form of calmodulin inhibited the receptor phosphorylation by GRK2.
(6) The role of each domain of calmodulin was investigated using chimera calmodulin. To exert calmodulin function, co-ordinated calcium binding to a pair of calcium-binding site were required.
(7) Possible role of calcineulin were examined. It was found that calcineulin may inhibit long term potentiation, a possible machinery of memory.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (126 results)

All Other

All Publications (126 results)

  • [Publications] E. DE Castro: "Regulation rhodopsin phosphorylation by a family of neuronal calcium sensors." Biochem. Biophys. Res. Commun.216. 133-140 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S. Kawamura: "photorecoptor pretein s26, a cone homologue of S-modulin in frog reting." J. Biol. Chem.271. 21359-21364 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kajimoto, Y.: "Delayed changes in neural visinin-like calcium-binding protein gene expression caused by acute phencyclidine administration." J. Neural Transm.100. 257-262 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ikegami, S.: "A facilitatory effect on the induction of long-term potentiation in vivo by chronic administration of antissense oligodexynucleotides against catalytic subunits of calclneurin." Mol. Brain Res.41. 183-191 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Oishi, K. J.: "Cloning and characterization of hscl^+, a heat shock cognate gene of the fission yeast Schizasaccharomyces pombe." Gene. 181. 45-49 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Bastianelli, E.: "Hippocalcin in rat retina. Comparison with calbindin-D28K, calretinin and neurocalcin." Exp. Eye Res.60. 257-266 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shimohama, S.: "Changes of neurocalcin, a calcium-binding protein, in the brain ofpatients with Alzheimer′s disease." Brain Res.716. 233-236 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Porteros, A.: "Neurocalcin immunoreactivity in the rat accessory olfactory bulb." Brain Res.729. 82-89 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K. Sanada: "Role of heterogeneous N-terminal acylation of recoverin in rhodcpsin phcsphorylation." J. Biol. Chem.270. 15459-15462 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] D. Kojima: "Molecular properties of chimetical mutants of Gecko blue and bovine rhodopsin." Biochemistry. 35. 2625-2629 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S. Nishimura: "Structural changes in the peptide in complex formation between actlrated rhcdopsin and transducin studied by FTIR spectrossopy." Biochemistry. 35. 13267-13271 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tanaka, H.: "MEKA/Phosducin attenuates hydrophobicity of transducin br subunits without binding to farnesyl moiety." Biochim. Biophys. Res. Comm.233. 587-591 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takeda, Y.: "A norneuronal isoform of cell adhesion molecule LI : Tissue-specific expression and functional analysis." J. Neurochem.66. 2338-2349 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S. Kayada: "Cloning and expression of frog rhodcpsin cDNA" Comp. Biochem. Physiol.110B. 599-604 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] D. Kojima: "Moleular properties of chimerical mutants of gecko blue and bovine rhodopsin." Biochemistry. 35. 2625-2629 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M. Ishikawa: "Lectin cytochemical analysis of glycoconjugates in photoreceptor cell membranes of Lampetra japonica." Vision Res.36. 1513-1520 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sanada, K.: "Calcium-bourd recoverin tergets rhodopsin kinase to membranes to inhibit rhodopsin phosphorylation." FEBS Letters. 384. 227-230 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okazaki, K.: "S100b is a target protein of neurocalcind, an isoform adundant in glial cells." Biochem. J.306. 551-555 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Moriya, M.: "Immunohistochemical localization of the calcium/calmodulin-dependent protein phosphatase, calcineurin, in the mouse tetis : its unique accumulation in spermatid nuclei." Cell Tissue Res.281. 273-281 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshino, H.: "Solution X-ray Scattering Data Show Structural Differences between Yeast and Veretebrate Calmodulin : Implications for Structure/Function." Biochemistry. 35. 2388-2393 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakashima, K.: "Chimeras of Yeast and Chicken Calmodulin Demonstrate Differences in Activation Mechanisms of Target Enzymes." Biochemistry. 35. 5602-2610 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S. Kawamura: "Guidebook to the calcium binding-Proteins. 分担執筆" Oxford University Press., 238(115-117) (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takamatsu, K :"Hand book to the Calcium-binding Protein. 分担執筆" Oxford University Press., 238(100-102) (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] E.De Castro, S.Nef, H.Fiumelli, S.E.Lentz, S.Kawamura, and P.Nef.: "Regulation of rhodopsin phosphorylation by a family of neuronal calcium sensors" Biochem.Biophys.Res.Commun.216. 133-140 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Kawamura.: "Unsolved issues in S-modulin/recoverin study." Behav.Brain Sci.18. 479-480 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Kawamura, O.Kuwata, M.Yamada, S.Matsuda, O.Hisatomi, and F.Tokunaga: "Photoreceptor protein s26, a cone homologue of S-modulin in frog retina" J.Biol.Chem.271. 21359-21364 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kajimoto, Y., Shirakawa, O., Kuno, T., Nishino, N.and Nakai, H.: "Delayd changes in neural visinin-like calcium-binding protein gene expression caused by acute phencyclidine administration" J.Neural Transm.100. 257-262 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Omay, S.B., Nakai, K., Kuno, T., Shiku, H.and Nishikawa, M.: "la, 25-dihydroxyvitamin D3-induced upregulation of calcineurin during leukemic HL-60 cell differentiation" Blood. 87. 2947-2955 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ikegami, S., Kato, A., Kudo, Y., Kuno, T., Ozawa, F.and Inokuchi, K.: "A facilitatory effect on the induction of long-term potentiation in vivo by chronic administration of antisense oligodeoxynucleotides against catalytic subunits of calcineurin." Mol.Brain Res.41. 183-191 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Oishi, K.-I., Sugiura, R., Shuntoh, H.and Kuno, T.: "Cloning and characterization of hsc1+, a heat shock cognate gene of the fission yeast Schizosaccharomyces pombe" Gene. 181. 45-49 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Toda, T., Dhut, S., Superti-Furga, G., Gotoh, Y., Nishida, E., Sugiura, R.and Kuno, T.: "The fission yeast pmk1+gene encodes a novel mitogen-activated protein kinase homolog which regulates cell integrity and functions coordinately with the protein kinase C pathway" Mol.Cell.Biol.16. 6752-6764 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Bastianelli, E., Takamatsu, K., Okazaki, K., Hidaka, H.and Pochet, R.: "Hippocalcin in rat retina. Comparison with calbindin-D28k, calretinin and neurocalcin" Exp.Eye Res.60. 257-266 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Saitoh, S., Kobayashi, M., Kuroki, T., Noguchi, T.and Takamatsu, K.: "The development of neural visinin-like Ca-binding protein 2 immunoreactivity in the rat neocortex and hippocampus" Neurosci.Res.23. 383-388 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takamatsu, K.: "A novel protein family of neuronal modulators." Behv.Brain Sci.18. 489-490 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takeda, Y., Asou, H., Murakami, Y., Miura, M., Kobayashi, M.and Uyemura, K.: "A nonneuronal isoform of cell adhesion molecule L1 : Tissue-specific expression and functional analysis" J.Neurochem.66. 2338-2349 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Kayada, O.Hisatomi and F.Tokunaga: "Cloning and expression of frog rhodopsin cDNA" Comp.Biochem.Physiol.110B. 599-604 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] I.Shimizu, M.Ueda, K.Sakamoto, O.Hisatomi and F.Tokunaga: "Comparison of DNA sequences of exon-4 or exson-5 in visual pigment genes between amphidromous and landlocked ayu-fish Plecoglossus altivelis" Proc.Jap.Acad. 71B. 84-86 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] F.Tokunaga and K.Yoshihara: "Structure and function ofretinochrome" Israel J.Chem.35. 357-364 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] O.Hisatomi, T.Satoh, L.Birthal, D.Stemkanp, P.Raymond and F.Tokunaga: "Molecular cloning and characterization of the putative ultraviolet-sensitive visual pigment of goldfish" Vision Res.36. 933-939 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] D.Kojima, T.Ohura, O.Hisatomi, F.Tokunaga, Y.Fukada, T.Yoshizawa and Y.Shichida: "Molecular properties of chimerical mutants of gecko blue and bovine rhodopsin" Biochemistry. 35. 2625-2629 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] D.l.Stemkamp, O.Hisatomi, K.L.Barthel, F.Tokunaga, and P.A.Raymond: "Temporal expression of rod and cone opsins in embryonic goldfish retina predicts the spacial organization to the cone mosaic" Invest.Ophthal.Vis.Sci.37. 363-376 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Kawamura, O.Kuwata, M.Yamada, S.Matsuda, O.Hisatomi and F.Tokunaga: "Photoreceptor protein s26, a cone homologue of S-modulin in frog retina" J.Biol.Chem.271. 21359-21364 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Ishikawa, A.Tonosaki, O.Hisatomi, F.Tokunaga, T.Koseki and S.Sakuragi: "Lectin cytochemical analysis of glycoconjugates in photoreceptor cell membranes of Lampetra japonica." Vision Res.36. 1513-1520 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sanada, K., Shimizu, F., Kameyama, K., Haga, K., Haga, T., and Fukada, Y.: "Calcium-bound recoverin targets rhodopsin kinase to membranes to inhibit rhodopsin phosphorylation" FEBS Letters. 384. 227-230 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Haga, K., Tsuga, H., and Haga, T.: "Ca, Q* {-dependent inhbition of G protein-coupled receptor kinase 2 by calmodulin." Biochemistry. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okazaki, K., Obata, N.H., Inoue, S.and Hidaka, H.: "S100b is a target protein of neurocalcin d, an isoform abundant in glial cells." Biochem.J.306. 551-555 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Junttila, T., Koistinaho, J., Rechardt, L., Hidaka, H., Okazaki, K.and P-Huikko, M.: "Localization of neurocalcin-like immunoreactivity in rat cranial motoneurons and spinal cord interneurons." Neurosci.Lett.183. 100-103 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okazaki, K., Iino, S., Kobayashi, S.and Hidaka, H.: "Effects on tubulin assembly of neurocalcin d, an isoform of 3EF hand calcium-binding protein in the mammalian nervous system" Life Sci.Adv.-Biochemistry. 14. 23-26 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Bastianelli, E., Polans, A.S., Hidaka, H.and Pochet, R.: "Differential distribution of six calcium-binding proteins in the rat olfactory epithelium during postnatal development and adulthood." J.Comp.Neurol.354. 395-409 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Bastianelli, E., Okazaki, K., Hidaka, H.and Pochet, R.: "Hippocalcin in rat retina. Comparison with calbindin-D28k, calretinin and neurocalcin." Exp.Eye Res.60. 257-266 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Iino, S., Kobayashi, S., Okazaki, K.and Hidaka, H.: "Neurocalcin-immunoreactive receptor cells in the rat olfactory epithelium and vomeronasal organ." Neurosci.Lett.191. 91-94 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Iino, S., Kobayashi, S., Okazaki, K.and Hidaka, H.: "Immunohistochemical localization of neurocalcin in the rat inner ear." Brain Res.680. 128-134 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Potts, B.C.M., Smith, J., Akke, M., Macke, T.J., Okazaki, K., Hidaka, H., Case, D.A.and Chazin, W.J.: "The structure of calcyclin reveals a novel homodimeric fold for S100 Ca-binding proteins." Natute Structural Biology. 2. 790-796 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shimohama, S., Chachin, M., Taniguchi, T., Hidaka, H.and Kimura, J.: "Changes of neurocalcin, a calcium-binding protein, in the brain of patients with Alzheimer's disease." Brain Res.716. 233-236 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Porteros, A., Brinon, J.G., Crespo, C., Okazaki, K., Hidaka, H., Aijon, J.and Alonso, J.R.: "Neurocalcin immunoreactivity in the rat accessory olfactory bulb." Brain Res.729. 82-89 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] D.Kojima, H., Imai, T.Okano, Y.Fukada, F.Crescitelli, T.Yoshizawa and Y.Shichida: "Purification and low temperature spectroscopy of gecko visual pigments green and blue." Biochemistry. 34. 1096-1106 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Sanada, T.Takao, Y.Shimonishi, T.Yoshizawa and Y.Fukada.: "Role of heterogeneous N-terminal acylation of recoverin in rhodopsin phosphorylation." J.Biol.Chem.270. 15459-15462 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Okano, Y.Fukada and T.Yoshizawa.: "Molecular basis for tetrachromatic color vision." Comp.Biochem.Physiol.112B. 405-414 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] R.Morishita, H.Nakayama, T.Isobe, T.Matsuda, Y.Hashimoto, T.Okano, Y.Fukada, K.Mizuno, S.Ohno, O.Kozawa, K.Kato and T.Asano: "Primary structure of gamma subunit of G protein, gamma12, and its phosphorylation by protein kinase C." J.Biol.Chem.270. 29469-29475 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Sanada and Y.Fukada.: "Unique lipids and unique properties of retinal proteins." Behavioral and Brain Sciences. 18. 486-487 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Ohguro, K.Kitamura, K.Konari, H.Sohma, Y.Fukada and T.Akino.: "The differences in the expression of visual pigments and transducin in photoreceptor cell differentiation." Tohoku J.Exp.Med.178. 233-240 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] O.Shouno, K.Kokame, M.Araki, T.Takao, Y.Shimonishi, M.Murata, T.Yoshizawa and Y.Fukada.: "Preparation and characterization of monoclonal antibodies specific for lauroylated isoform of bovine transducin alpha-subunit : Immunohistochemical analysis of bovine retinas." J.Neurochem.66. 2188-2196 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] D.Kojima, T.Oura, O.Hisatomi, F.Tokunaga, Y.Fukuda, T.Yoshizawa and Y.Shichida.: "Molecular properties of chimerical mutants of Gecko blue and bovine rhodopsin." Biochemistry. 35. 2625-2629 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Sanada, F.Shimizu, K.Kameyama, K.Haga, T.Haga and Y.Fukada.: "Calcium-bound recoverin targets rhodopsin kinase to membranes to inhibit rhodopsin phosphorylation." FEBS Lett.384. 227-230 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Tanaka, C.-H.Kuo, T.Matsuda, Y.Fukada, F.Hayashi, Y.Ding, Y.Irie and N.Miki.: "MEKA/Phosducin attenuates hydrophobicity of transducin beta/gamma-subunits without binding to farnesyl moiety." Biochem.Biophys.Res.Commun.223. 587-591 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Nishimura, J.Sasaki, H.Kandori, T.Matsuda, Y.Fukada and A.Maeda.: "Structural changes in the peptide backbone in complex formation between activated rhodopsin and transducin studied by FTIR spectroscopy." Biochemistry. 35. 13267-13271 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Okano, K.Yamazaki, T.Kasahara and Y.Fukada.: "Molecular cloning of heterotrimeric G-protein alpha-subunits in chicken pineal gland." J.Mol.Evol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Hirunagi, S.Ebihara, T.Okano, Y.Takanaka and Y.Fukada.: "Immuno-electron microscopic investigation for the subcellular localization of pinopsin in the pineal organ of the chicken." Cell Tissue Res.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Okano and Y.Fukada.: "Phototransduction cascade and circadian oscillator in chicken pineal gland." J.Pineal Res.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tanaka, H., Iwami, C., Kuo, C-H., Ding, Y., Do, Y., Irie, Y.and Miki, N.: "Analysis of Tbetagamma-binding domain of MEKA/phosducin." Neurochem.Int.(in press). (1977)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tanaka, H., Kuo, C-H., Matsuda, T., Fukada, Y., Hayashi, F., Ding, Y., Irie, Y.and Miki, N.: "MEKA/Phosducin attenuates hydrophobicity of transducin br subunits without binding to farnesyl moiety." Biochim.Biophys.Res.Comm.233. 587-591 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kimura, E., Matsuura, I., Tai, K., Nakashima, K.and Yazawa, M.: "The C-terminal Half-molecular Domain of Calmodulin is Responsible for High-affinity Interaction with Target Enzymes." Proc.Japan Acad.71B. 293-298 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Moriya, M., Fujinaga, K., Yazawa, M., and Katagiri, C.: "Immunohistochemical localization of the calcium/calmodulin-dependent protein phosphatase, calcineurin, in the mouse testis : its unique accumulation in spermatid nuclei." Cell Tissue Res.281. 273-281 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ohki, S., Miura, K., Saito, M., Nakashima, K., Maekawa, H., Yazawa, M., Tsuda, S.and Hikichi, K.: "Secondary Structure and Ca, Q* {-binding Property of the N-terminal Half Domain of Calmodulin from Yeast Saccharomyces cerevisiae as Studied by NMR." J.Biochem.119. 1045-1055 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Aramini, J.A., Hiraoki, T., Yazawa, M., Yuan, T., Zhang, M.and Vogel, H.J.: "Lead-207 NMR : a novel probe for the study of calcium-binding proteins." J.Bioinorg.Chem.1. 39-48 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshino, H., Izumi, Y., Sakai, K., Takezawa, H., Matsuura, I., Maekawa, H.and Yazawa, M.: "Solution X-ray Scattering Data Show Structural Differences between Yeast and Vertebrate Calmodulin : Implications for Structure/Function." Biochemistry. 35. 2388-2393 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakashima, K., Maekawa, H., and Yazawa, M.: "Chimeras of Yeast and Chicken Calmodulin Demonstrate Differences in Activation Mechanisms of Target Enzymes." Biochemistry. 35. 5602-5610 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Kawamura: Phototransduction, excitation and adaptation. in Neurobiology of the outer retina. ed.by Archer, S., Djamgoz, M.and Vallerga, S.Chapman & Hall, London, 105-131 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Kawamura: S-modulin. in Guidebook to the calcium binding-Proteins. ed.by Celio, M.R.Oxford University Press, 115-117 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takamatsu, K., Saitoh, S.and Kobayashi, M.: Hippocalcin. Handbook to the Calcium-binding Protein (Celio, M.ed).Oxford University Press., 100-102 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Fukada.: Prenylation and carboxylmethylation of G protein gamma subunit. in : "Methods in Enzymology" Vol.250, "Lipid Modifications of Proteins" (Casey and J.E.Buss, eds). Academic Press, 91-105 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Kawamura: "photoreceptor protein s26,a cone homo logue of S-modulin in-frog retina." J.Biol.Chem.271. 21359-21364 (1996)

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      1996 Annual Research Report
  • [Publications] Ikegami,S.: "A focilitatory effect on the induction of long-term potentiation in vivo by chronic administration of antisense oligodeoxynucleotides against catalytic subunits of calcineurin." Mol.Brain Res.41. 183-191 (1996)

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      1996 Annual Research Report
  • [Publications] Oishi,K,I.: "Cloning and characterization of hsc 1^+,a heat shock cognate gene of the fission yeast Schizosaccharomyces pombe." Gene.181. 45-49 (1996)

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      1996 Annual Research Report
  • [Publications] Takeda,Y.: "A nonneuronal isoform of cell adhesion molecule L1:Tissue-specific expression and functional analysis." J.Neurochem.66. 2338-2349 (1996)

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      1996 Annual Research Report
  • [Publications] D.Kojima: "Molecular properties of chimerical mutants of gecko blue and bovine rhodopsin." Biochemistry. 35. 2625-2629 (1996)

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      1996 Annual Research Report
  • [Publications] M.Ishikawa: "Lectin cytochemical analysis of glycoconjugates in photoreceptor cell membranes of Lampetra japonica." Vision Res.36. 1513-1520 (1996)

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      1996 Annual Research Report
  • [Publications] Sanada,K.: "Calcium-bound recoverin targets rhodopsin kinase to membranes to inhibit rhodopsin phosphorylation." FEBS Letters. 384. 227-230 (1996)

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      1996 Annual Research Report
  • [Publications] Shimohama,S.: "Changes of neurocalcin,a calcium-binding protein,in the brain of patients with Alzheimer's disease." Brain Res.716. 233-236 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Porteros,A.: "Neurocalcin immunoreactivity in the rat accessory olfactory bulb." Brain Res.729. 82-89 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] D.Kojima: "Molecular properties of chinerical mutants of Gecko blue and bovine rhodopsin." Biochemistry. 35. 2625-2629 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] S.Nishimura: "Structural changes in the peptide backbone in complex formation between activated rhodopsin and transducin studied by FTIR spectroscopy." Biochemistry. 35. 13267-13271 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tanaka,H.: "MEKA/Phosducin attenuates hydrophobicity of transducin by subunits without binding to farnesyl moiety." Biochem.Biophys.Res,Comm.233. 587-591 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yoshino,H.: "Solution X-ray Scattering Data Show Structural Differences between Yeast and Vertebrate Calmodulin:Implications for Structure/Function." Biochemistry. 35. 2388-2393 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nakashima,K.: "Chimeras of Yeast and Chicken Calmodulin Demonstrate Differences in Activation Mechanisms of Target Enzymes." Biochemistry. 35. 5602-5610 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] S.Kawamura: "Guidebook to the calcium binding-Proteins(分担執筆pp115-pp117)" Oxford University Press., 238 (1996)

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      1996 Annual Research Report
  • [Publications] Takamatsu,K.: "Handbook to the Calcium-binding Protein(分担執筆pp100-pp102)" Oxford University Press., 238 (1996)

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      1996 Annual Research Report
  • [Publications] De Castro,E.: "Regulation of rhodopsin phosphorylation by a family of neuronal calcium sensors." Biochem.Biophys.Res.Commun.216. 133-140 (1995)

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      1995 Annual Research Report
  • [Publications] 河村 悟: "視覚中枢の分子機構-網膜-" 分子神経科学の最先端. 135-145 (1995)

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      1995 Annual Research Report
  • [Publications] Kawamura,S.: "Unsolved issues in S-modulin /vecoverin study." Behav.Brain Sci.18. 479-480 (1995)

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      1995 Annual Research Report
  • [Publications] Kajimoto,Y.: "Delayed changes in neural visinin-like calcium-binding protein gene expression caused by acute phencyclidineadministration." J.Neural Transm.100. 257-262 (1995)

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      1995 Annual Research Report
  • [Publications] Cmay,S.B.: "1α,25-dihydroxyvitamin D3-induced upregulation of calcineurin during leukemic HL-60 cell differentiation." Blood. 87(in press). (1996)

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      1995 Annual Research Report
  • [Publications] Kuno,T.: "Purification and activation of cyclic GMP-dependent protein Kinase." Methods in molecular biology. 41. 123-127 (1995)

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      1995 Annual Research Report
  • [Publications] Bastianelli,E.: "Hippocalcin in rat retina.Comparison with calbindin-D28K,calvetinin and neurocalcin." Experimental Eye Research. 60. 257-266 (1995)

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      1995 Annual Research Report
  • [Publications] Takamatsu,K.: "A novel protein family of nenronal modulators." Behavioral and Brain Sciences. 18. 489-490 (1995)

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      1995 Annual Research Report
  • [Publications] Saitoh,S.: "The development of neural visinin-like Ca^<2+>-binding protein Zimmunoreactivity in the rat neocortex and hippocampus." Neuroscience Research. 23. 383-388 (1995)

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      1995 Annual Research Report
  • [Publications] Seiya Kayada: "Cloning and Expression of Frog Rhodopsin c DNA" Comparative Biochemistry and Physiology. 110B. 599-604 (1995)

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      1995 Annual Research Report
  • [Publications] Osamu Hisatomi: "Molecular Cloning and Characterization of the Putative Ultraviolet-Sensitive Visual" Vision Research. 20(in press). (1995)

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      1995 Annual Research Report
  • [Publications] 徳永史生: "動物の色覚" 科学. 65. 485-492 (1995)

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      1995 Annual Research Report
  • [Publications] Kuo,c-H.: "Determination of a necdin cis-acting element required for neuron specific expression by using zebra fish." Biochem.Biophys.Res.Comm.211. 438-446 (1995)

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      1995 Annual Research Report
  • [Publications] Taira,E.: "Expression and functional analysis of a novel isoform of gicerin,an immunoglobulin superfamily cell adhesion molecule." J.Biol.Chem.270. 28681-28687 (1995)

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      1995 Annual Research Report
  • [Publications] Ikemoto,M.: "Decrease in CRE-binding activity by chronic morphine administration in mouse brain." Neuro Report. 6. 262-264 (1995)

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      1995 Annual Research Report
  • [Publications] K.Okazaki.: "Siooβ is a target protein of neurocalcin δ,an isoform abundant in glial cells." Biochemical Journal. 306. 551-555 (1995)

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      1995 Annual Research Report
  • [Publications] T.Junttila.: "Localization of neurocalcin-like immunoreactivity in rat cranial motoneurons and spinal cord interneurons." Neuroscience Letters. 183. 100-103 (1995)

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      1995 Annual Research Report
  • [Publications] B.C.M.Potts: "The structure of calcyclin reveals a novel homodimeric fold for S100 Ca^<2+>-binding proteins." Natute Structural Biology 2. 790-796 (1995)

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      1995 Annual Research Report
  • [Publications] Nakamura,F.: "Characterization of G9 family G proteins,GL1alpha(G14alpha),GL2alpha(G11alpha),and G9alpha expressed in the baculovirus-insect cell system." J.Biol.Chem.270. 6246-6253 (1995)

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      1995 Annual Research Report
  • [Publications] Shui,Z.: "Receptor kinase-dependent desensitization of the muscarinic K^+ current in rat atrial cells." J.Physiol.(Lond.). 487. 359-366 (1995)

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      1995 Annual Research Report
  • [Publications] Haga,K.: "Phosphorylation of human m1 muscarinic acetylcholine receptors by G proteincoupled receptor kinase 2 and protein kinase C." J.Biol.Chem.271. 2776-2782 (1996)

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      1995 Annual Research Report
  • [Publications] Kamon Sanada: "Unique lipids and unique properties of retinal proteins." Behavioral and Brain Sciences. 18. 486-487 (1995)

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      1995 Annual Research Report
  • [Publications] Kamon Sanada: "Role of heterogeneous N-terminal acylation of recoverin in rhodopsin phosphorylation." J.Biol.Chem.270. 15459-15462 (1995)

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      1995 Annual Research Report
  • [Publications] Osamu Shono: "Preparation and characterization of monoclonal antibodies specific for lauroylated isoform of bovine transducin α-subunit." J.Neurochem. (in press). (1996)

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      1995 Annual Research Report
  • [Publications] T.Katoh: "Evidence for Involvement of a 12-Residue Peptide Segment of the Heavy Chain in the Neck Region of Smooth Muscle Myosin in Formation of the 10S Conformation" J.Biochem.118. 428-434 (1995)

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      1995 Annual Research Report
  • [Publications] M.Moriya: "Immunohistochemical localization of the calcium/calmodulin-dependentprotein phosphatase,calcineurin,in the mouse testis:its unique accumulationin spermatid nuclei." Cell Tissue Res.281. 273-281 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Yoshino: "Solution X-ray Scattering Data Show Structural Differences between Yeast and Vertebrate Calmodulin:Implications for Structure/Function." Biochemistry. 35(in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Kawamura,S.: "Neurobiology of the outen retina,(分担執筆)" Chapman & Hall,London., 501(105-131) (1995)

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      1995 Annual Research Report
  • [Publications] Mukai,H.: "Guidebook to Molecular Chaperones and Protein Folding Catalysts" Oxford Univ.Press(in press), (1996)

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      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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