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Molecular Ecological Studies for Bioremediation of Aquatic Environments by Killing Factors and/or Self-Death-Inducing Factors from Microorganisms

Research Project

Project/Area Number 07406010
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

NAKAHARA Hiroyuki (1996-1997)  Kyoto Univ., Graduate Sschool of Agriculture, Professor, 農学研究科, 教授 (80026567)

石田 祐三郎 (1995)  京都大学, 農学部, 教授 (20026488)

Co-Investigator(Kenkyū-buntansha) IMAI Ichiro  Kyoto Univ., Graduate Sschool of Agriculture, , Associate Professor, 農学研究科, 助教授 (80271013)
FURUSAWA Iwao  Kyoto Univ., Graduate Sschool of Agriculture, Professor, 農学研究科, 教授 (10026594)
YOSHINAGA Ikuo  Kyoto Univ., Graduate Sschool of Agriculture, Instructor, 農学研究科, 助手 (40230776)
SAKO Yoshihiko  Kyoto Univ., Graduate Sschool of Agriculture, Associate Professor, 農学研究科, 助教授 (60153970)
UCHIDA Aritsune  Kyoto Univ., Graduate Sschool of Agriculture, Professor, 農学研究科, 教授 (50027190)
本城 凡夫  水産庁南西海区水産研究所, 部長
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥32,100,000 (Direct Cost: ¥32,100,000)
Fiscal Year 1997: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1996: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1995: ¥22,300,000 (Direct Cost: ¥22,300,000)
Keywordsred tide / algicidal bacteria / marine virus / 16SrDNA / RFLP / Heterosigma akashiwa / Gymnodinium mikimotoi / Alexanbdrium tamarense / Heterosigama akashiwa / Gymnodinium mikimotoi / 海洋性ウィルス / Heterosigma akashiwo / Gymnodinium属 / Heterosigma属 / 海洋ウィルス / 16SrRNA
Research Abstract

We enumerated the microorganisms which killed the noxious red tide phytoplankton Heterosigma akashiwo (raphidophyceae) and Gymnodinium mikimotoi, (dinophyceae) ハ during their blooms which occurred in Hiroshima Bay and Uranouchi Bay or Tanabe Bay, Japan, in 1995 and 1996, using the MPN method.
At every sampling site in Hiroshima Bay in both years, the rapid increase of the algicidal microorganisms in the seawater fractions less than 0.8mm was observed at the termination period of the blooms. Because the seawater fraction less than 0.2mm fluctuated poorly related to the extinction of H.akashiwo blooms, the algicidal bacteria may play the leading part in the termination of the bloom. We could isolate many strains of H.akashiwo-killing bacteria (HAKB) during the blooms. The number of algicidal bacteria against Chattonella antiqua were one or two order of magnitude lower than that of HAKB.Therefore it is suggested that the population dynamics of algicidal bacteria may have a close relationsh … More ip to the blooms of the phytoplankton. In marine ecosystem, algicidal bacteria against specific phytoplankton seem to regulate the change of structure of phytoplankton communities.
Several strains of the marine bacteria which killed G.mikimotoi were silated during the occurrenece of the huge red tide of G.mikimotoi in Tanabe Bay. Among them the strains belonged to the genus Vibrio, Flavobacterium, Acinetobacter and Pseudomonas-Alteromonas. The fact that all of them grew in filtered natural seawater without any nutrient supplements suggested that the killing bacteria are ubiquitous in seawter and are related to the occurrence of G.mikimotoiopportunistically. Most of the killing bacteria did not affect the growth of three marine diatoms, Skeletonema costatum, Ditylum brighwelli and Thalassiosira sp., and some of them did not affect the growth of the Alexandrium catenella.
A bacterium, Flavobacterium sp.5N-3, obtained from sawater after the disappearance of a noxious G.mikimotoi red tide in Uranouchi Bay showed a drastic algicidal effect on this species in particular, while it had no effects on other red tide plankton of Skeketonem costatum, Heterosigma akashiwo, and chattonella anitiqua.
These results suggest that bacteria with species-specific inhibitory effects play an importnat role on the succession of phytoplankton community and algal blooming could be prevented by using such microbes as a "microbial pesticide". Less

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (48 results)

All Other

All Publications (48 results)

  • [Publications] I.Yoshinaga et al.: "Analysis of algicidal ranges of the bacteria killing the marine dinoflagellate Gymnodiniummikimotoi isolted from Tanabe Bay,Wakayama Pref.,Japan" Fisheries Science. 63. 94-98 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 左子 芳彦: "モノクローナル抗体およびリボソーマルRNA遺伝子による有毒微細藻の分子識別" 海洋. 29. 725-7312 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Adachi et al.: "Analysis of Gymnodiniumcatenatum Dinophyceae using sequences of the 5.8SrDNA-ITS regions and radom amplified polymorphicDNA" Fisheries Science. 63. 701-731 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Yoshikawa et al.: "Molecular cloning and uncleotide sequence analysis of the gene coding for chloroplast-type ferredoxin from the dinoflagellates Peridinium bipes and Alexandrium tamarense." Fisheries Science. 63. 692-700 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 吉永 郁生: "赤潮の動態に関わる従属栄養細菌の分子生態学的研究" 日本水産学会誌. 63. 502-505 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Shin et al.: "Phylogenetic analysis by 16SrRNA gene seqauncing of obligate oligotrophs isolated from the Northern Basin of Lake Biwa (mesotrophic lake)." Microbes and Environments. 12. 27-36 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Nagasaki et al: "The disintegration process of a Heterosigma akashiwo (Raphidophycea) red tide in northern-Hiroshima Bay,Japan,during the summer of 1994" Harmful and Toxic Algal Blooms. 7. 251-254 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Nakanishi et al.: "Detection of the red tide-causing plankton Chattonella marina using a piezoelectric immunosensor" Analytica Chimica Acta. 325. 73-80 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Sako et al.: "Phylogenetic relationship of ciguatera-causing dinoflagellate Gambierdiscus toxicus with 18S ribosomal DNA sequence comparison." Harmful and Toxic Algala Blooms. 7. 443-446 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] I.Yoshinaga et al.: "Analysis of algicidal ranges of the bacteria killing the marine dinoflagellate Gymnodiniummikimotoi isolted from Tanabe Bay,Wakayama Pref.,Japan" Fisheries Science. 63. 94-98 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Sakata et al.: "Isolation of algophorous Labyrinthura and Amoebae from marine habitas" Mem.Fac.Kagoshima Univ.45. 13-19 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Fukami et al.: "Effect of bacterial assemblages on the succession of blooming phytoplankton from Skeletonema contatum to Heterosigma akashiwo" Harmful and Toxic Algala Blooms. 7. 335-338 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Ishida et al.: "Algal Blooms,and Toxins" Progress in Microbial Ecology, 568 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Uchida et al.: "Environmental and Biological Chemistry of Dimethylsulfoniopropinate and Related Sulfonium Compounds" Plenum Press New York, 364 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Nagasaki et al.: "The disintegration process of a Heterosigma akashiwo (Raphidophycea) red tide in northern Hiroshima Bay, Japan, during the summer of 1994" Harmful and Toxic Algal Blooms. 7. 251-254 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Nakanishi et al.: "Detection of the red tide-causing plankton Chattonella marina using a piezoelectric immunosensor" Analytica Chimica Acta. 325. 73-80 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Sako et al.: "Phylogenetic relationship of ciguatera-causing dinoflagellate Gambierdiscus toxicus with 18S ribosomal DNA sequence comparison." Harmful and Toxic Algala Blooms. 7. 443-446 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] I.Yoshinaga et al.: "Analysis of algicidal ranges of thebacteria killing the marine dinoflagellate Gymnodinium mikimotoiisolted from Tanabe Bay, Wakayama Pref., Japan" Fisheries Science. 63-1. 94-98 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Sakata et al.: "Isolation of algophorous Labyrinthura and Amoebae from marine habitas" Mem.Fac.Kagoshima Univ.45. 13-19 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Fukami et al.: "Effect of bacterial assemblages on the succession of blooming phytoplankton from Skeletonema contatum to Heterosigma akashiwo" Harmful and Toxic Algala Blooms. 7. 335-338 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] I.Yoshinaga et al.: "Analysis of algicidal ranges of the bacteria killing the marine dinoflagellate Gymnodinium mikimotoi" Fisheries Science. 63. 94-98 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.adachi et al.: "Analysis of Gymnodinium catenatum Dinophycea using seauences of the 5.8S rDNA-ITS regions and random amplified polymorphic DNA" Fisheries Science. 63(5). 701-707 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Yoshikawa et al.: "Molecular cloning and nucleotide sequence analysis of the gene coding for chloroplast-type ferredoxin from the dinoflagellates Peridinium bipes and Alexandrium tamarense" Fisheries Science. 63(5). 692-700 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Shin et al: "Phylogenetic analysis by 16S rRNA gene sequencing of obligate oligotrophs isolated from the Northern Basin of Lake Biwa (mesotrophic lake)" Microbes and Environments. 12(2). 27-36 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Ishida et ak: "Algala Blooms and Toxins" Progress in Microbial Ecology. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Uchida et al.: Environmental and Bioligical Chemistry of Dimethylsulfoniopropionate and Related Sulfonium Compounds. Plenm Press New York, 364 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] I.Yoshinaga et al.: "Analysis of algicidal ranges of thebacteria killing the marine dinoflagellate Cymnodinium mikimotoi isolted from Tanade Bay,Wakayama Pref.,Japan" Fisheries Science. 63. 94-98 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 左子芳彦: "モノクローナル抗体およびリボソーマルRNA遺伝子による有毒微細藻の分子識別" 海洋. 29. 725-731 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Adachi et al: "Analysis of Gymnodinium catenatum Dinophyceae using sequences of the 5.8S rDNA-ITS regions and random amplified polymorphic DNA" Fisheries Science. 63.5. 701-731 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Yoshikawa et al.: "Molecular cloning and nucleotide sequence analysis of the gene coding for chloroplast-type ferredoxin from the dinoflagellates Peridinium bipes and Alexandrium tamarense." Fisheries Science. 63.5. 692-700 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 吉永郁生: "赤潮の動態に関る従属栄養細菌の分子生態学的研究" 日本水産学会誌. 63.4. 502-505 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Shin et al.: "Phylogenetic analysis by 16S rRNA gene seqauncing of obligate oligotrophs isolated from the Northern Basin of Lake Biwa (mesotrophic lake)." Microbes and Environments. 12.2. 27-36 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Ishida et al: "Algal Blooms,and Toxins" Progress in Microbial Ecology, 568 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Nagasaki et al.: "The disintegration process of a Heterosigma akashiwo (Raphidophyes) red tide in northern Hiroshima Bay,Japan,during the summer of 1994" Harmful and Toxic Algal Blooms. 7. 251-254 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Nakanishi et al.: "Detection of the red tide-causing plankton Chattonella marina using a piezoelectric immunosensor" Analytica Chimica Acta. 325. 73-80 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Sako et al.: "Phylogenetic relationship of ciguatera-causing dinoflagellate Gambierdiscus toxicus with 18S ribosomal DNA sequence comparison." Harmful and Toxic Algal Blooms. 7. 443-446 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Sako et al.: "Detection of the toxic dinoflagellates Alexandrium species by flow cytometry using a monoclonal antibody." Harmful and Toxic Algal Blooms. 7. 463-466 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Yoshida et al.: "Purification and properties of paralytic shellfish poisoning toxins sulforansferase from toxic dinoflagllate Gymnosinium catenatum." Harmful and Toxic Algal Blooms. 7. 499-502 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] I.Yoshinaga et al.: "Analysis of algicidal ranges of thebacteria killing the marine dinoflagellate Gymnodiniummikimotoi isolted from Tanabe Bay,Wakayama Pref.,Japan" Fisheries Science. 63・1. 94-98 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] A.Uchida et al.: "Plenum Press New York" Environmental and Biological Chemistry of Dimethylsulfoniopropionate and Related Sulfonium Compounds, 364 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nagasaki, K. et al.: "Virus-like particles in unicellular apochlorotic microorganisms in the coastal water of Japan" Fisheries Science. 61. 235-239 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nagasaki, K. et al.: "Viral infection in Heterosigam akashiwo (Raphidophyceae) : a possible termination mechanism of the noxiousredtide" Harmful Marine Algal Blooms. 639-644 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoshinaga, I. et al.: "Lysis of Gymnodinium nagasakiense by marine bacteria" Harmful Marine Algal Blooms. 687-694 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Imai, I. et al.: "Algicidal marine bacteria isolated from Northern Hiroshima Bay, Japan" Fisheries Science. 61. 628-636 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoshinaga, I. et al.: "Distribution and fluctuation of bacteria inhibiting the growth of a marine red tide phytoplankton Gymnodinium mikimotoi in Tanabe Bay (Wakayama Pref., Japan)" Fisheries Science. 61. 780-786 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 吉永郁生: "田辺湾から分離した有害赤潮藻Gymnodinium mikimotoi殺滅細菌E401株の分類学的性状およびその殺滅機作" 環境科学総合研究所年報. 14. 47-58 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 石田祐三郎: "水産学シリーズ99 赤潮と微生物-環境と微生物農薬を求めて" 恒星社厚生閣, 126 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ishida, Y. et al: "Molecular Approaches to Food Safety Issues Involving toxic Microorganisms" Alaken Inc., 532 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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