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Dynamic analysis of crystal transformation of cellulose

Research Project

Project/Area Number 07406018
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 林産学
Research InstitutionThe University of Tokyo

Principal Investigator

OKANO Takeshi  Department of Biological Materials Science, Graduate School of Agriculture and Life Science, The University of Tokyo Professor, 大学院・農学生命科学研究科, 教授 (30011927)

Co-Investigator(Kenkyū-buntansha) SAMEJIMA Masahiro  Department of Biological Materials Science, Graduate School of Agriculture and L, 大学院・農学生命科学研究科, 助教授 (30162530)
WADA Masahisa  Department of Biological Materials Science, Graduate School of Agriculture and L, 大学院・農学生命科学研究科, 助手 (40270897)
KUGA Shigenori  Department of Biological Materials Science, Graduate School of Agriculture and L, 大学院・農学生命科学研究科, 助教授 (60012051)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥4,100,000 (Direct Cost: ¥4,100,000)
Keywordscrystal transformation / cellulose / ramie / poisson / crystal elastic modulus / cellulose I-alpha / rellulose I-beta / X-ray diffraction / セルロースIα / セルロースIβ / セルロースミクロフィブリル / アルカリセルロース / バクテリアセルロース / 加水分解 / セルラーゼ / イメージングプレート / 木材セルロース / 電子回折 / 酵素 / バロニア / 結晶素
Research Abstract

(1) A highly oriented film of microcrystalline Cladophora cellulose was heated in helium atmosphere, resulting in the transformation of cellulose Ia to cellulose Ib. This process was followed by a position-sensitive proportional counter (PSPC) and the change in the crystal lattice was successfully recorded. While the exact molecular mechanism of the transformation from cellulose Ia to Ib is not elucidated, our present results will give an important clue to determine whether it involves the rotation of molecular chain.
(2) The use of PSPC allowed us to determine simultaneously the deformation of crystal lattice of ramie fiber in both lateral and longitudinal directions. A bundle of 100 ramie fibers was subjected to tensile stress by using the fiber-tensioning device, and a load elongation chart was obtained by recording the three major equatorial reflections with PSPC.Analysis of similarly obtained load-elongation data for the meridional reflection gave longitudinal elastic modulus and poisson ratio of cellulose crystal, 129Gpa and 0.34, respectively.
(3) Swelling of ramie fiber by aq.Sodium hydroxice was studied by the use of PSPC and imaging plate (IP), resulting in new information. When aq.NaOH was added in 50 uL aliquots of to a bundle of 400 remie fibers, the change in three major equatorial reflections could be followed simultaneously. Comparison of the change in integral intensity led to the conclusion that the change in crystal lattice does not proceed isotropically but the change in a-axis direction precedes those in other directions, i.e., the hydrogen-bonded molecular sheet in the ab plane appears to be peeled off from the crystallite.
(4) cellulose-calcium thiocyanate complex was found to form cellulose I by regenerating in water. This complex was found to be a well-defined fibrous crystal, which is remarkably different from that of alkali cellulose.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Ishikawa, M.Wada, K.Igarashi, S.Kuga, T.Okano: "Effect of cellulase treatment ontensile properties of M.Samejima,ramie fiber" Mokuzai Gakkaishi. 43. 337-341 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Nishiyama, S.Kuga, M.Wada, T.Okano: "Cellulose microcrystal film of high uniaxial orientation" Macromolecules. 30. 6395-6397 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Wada, J.Sugiyama and T.Okano: "Synchrotron-radiated x-ray and neutron diffraction study of native cellulose" Cellulose. 4. 221-232 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Hayashi, J.Sugiyama, T.Okano, M.Ishihara: "The enzymatic susceptibility of cellulose microfibrils of the algal-bacterial type and the cotton-ramie type" Carbohydrate Research. 350. 109-117 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Habu, K.Igarashi, M.Samejima, B.pettersson and K-E.L.Eriksson: "Enhanced production of cellobiose dehydrogenase in cultures of Phanerochaete chrysosporium supplemented with bovine calf serum" Biotechnol.Appl.Biochem. 26. 97-102 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Shidazaki, M.Saito, S.Kuga, T.OKANO: "Native cellulose II production by A.Xylinum under physical conolition" Cellulose. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Ishikawa, M.Wada, K.Igarashi, S.Kuga, T.Okano, M.Samejima.: "Effect of cellulase treatment on tenislie properties oframie fiber" Mokuzai Gakkaishi. 43. 337-341 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Nishiyama, S.Kuga, M.Wada, T.Okano: "Cellulose microcrystal film of high uniaxial orientation" Macromolecules. 30. 6395-6397 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Wada, J.Sugiyama, T.Okano: "Synchrotron radiated X-ray and neutron diffraction study of native cellulose" Cellulose. 4. 221-232 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Hayashi, J.Sugiyama, T.Okano, M.: "Ishhihara : Ehe Enzymatic susceptibility of cellulose microfibrils of the algal-bacterial type and the cotton-ramie type." Carbohydrate Res. 350. 109-117 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Habu, K.Igarashi, M.Samejima, B.Petterson, K-E.L Eriksson: "Enhanced production of cellobiose dehydrogenase in clutures of Phanerochaete chrysosporium supplenented with bovine calf serum" Biotechnol.Appl.Biochem.26. 97-102 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Shibazaki, M.Saito, S.Kuga, T.Okano: "Native cellulose II production by A.xylinum under Physical condition" Cellulose. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ishikawa, M.Wada, K.Igarashi, S.Kuga, T.Okano: "Effect of cellulase treatment on tensile properties of M.Samejima,ramie fiber" Mokuzai Gakkaishi. 43. 337-341 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Nishiyama, S.Kuga, M.Wada, T.Okano: "Cellulose microcrystal film of hifh uniaxial orientation" Macromolecules. 30. 6395-6397 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Wada, J.Sugiyama and T.Okano: "Synchrotron-radiated x-ray and neutron diffraction study of native cellulose" Cellulose. 4. 221-232 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] N.Hayashi, J.Sugiyama, T.Okano, M.Ishihara: "The enzymatic susceptibility of cellulose microfibrils of the algal-bacterial type and the cotton-ramie type" Carbohydrate Research. 350. 109-117 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] N.Habu, K.Igarashi, M.Samejima, B.Pettersson and K-E.L.Eriksson: "Enhanced production of cellobiose dehydrogenase in cultures of Phanerochaete chrysosporium supplemented with bovine calf serum" Biotechnol.Appl.Biochem.26. 97-102 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Shibazaki, M.Saito, S.Kuga, T.OKANO: "Native cellulose II production by A.Xylinum under physical condition" Cellulose in press. (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Shibazaki,S.Kuga T.Okano: "Mercerization and acid hydrolysis of bacterial cellulose" Cellulose. 4(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Samejima: "Research trend in cellulase and cellulose biodegradation" Mokuzai Gakkaishi. 42. 811-816 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Samejima et al.: "Absorption behavior of cellulose dehydrogenase from phanerochaete chrisosporium to crystalline and amorphous cellulose" Biotechnol.Appl.Biochem.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Samejima et al.: "The use of cellobiose dehydrogenase as a sensitive detector of cellulase a ctivity" Proceedings of Sixth IC on BPPI. 181-184 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] A.Ishikawa et al.: "Effects of cellulase treatment on tensite properties of ramie fibers" Mokuzai Gakkaishi. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Igarashi et al.: "Localization of cellobiose dehydrogenase in cellulose-grown culture of phanerochaete chrysosporium" Fungal Genetics an Biology. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Wada,T.Okano,J.Sugiyama,F.Horii: "Characterization of tension and mormally lignified wood cellsilose in populus Maxirnowiczii." Cellulose. 2. 223-233 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Wada,T.Okano,J.Sugiyama: "Characterization of cellulose in wood." Proceedings of the ISTAWF. 234-238 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Samejima et al.: "The use of cellobiose dehydrogenase as a sensitive detecter of cellulose activity." Proceedings of 6th ICBPPI. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] S.Kuga et al.: "Beta-glucan synthesis in the cotton fiber." Plant Physiol.107. 111-123 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] S.Kuga et al.: "Acid hydrolysis behavior of mierovial cellulose II." Polymer. 36. 4971-4976 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Saito,T.Okano,H.Chanzy,J.Sugiyama: "Structural study of α-chutin from the grasping spine of the arrow worm" J.of Structural Biology. 114. 218-228 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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