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Molecular anatomy of functional structural expression of gap junctions.

Research Project

Project/Area Number 07407001
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKyushu University

Principal Investigator

SHIBATA Yosaburo  Kyushu Univ.Dept.Anatomy Professor, 医学部, 教授 (90037482)

Co-Investigator(Kenkyū-buntansha) NISHII Kiyomasa  Kyushu Univ.Dept.Anatomy Research Associate, 医学部, 助手 (20264020)
INAI Tetsuichiro  Kyushu Univ.Dept.Anatomy Research Associate, 医学部, 助手 (00264044)
NAKAMURA Kei-ichiro  Kyushu Univ.Dept.Anatomy Associate Professor, 医学部, 助教授 (20172398)
倉岡 晃夫  九州大学, 医学部, 助手 (30253412)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥31,900,000 (Direct Cost: ¥31,900,000)
Fiscal Year 1997: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1996: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1995: ¥24,300,000 (Direct Cost: ¥24,300,000)
Keywordsgap junction / connexin / Cx43 / Cx45 / Cx40 / Cx37 / phosphorylation / intestinal pacemaker / 小腸ペースメーカー / 血管内皮 / 小腸 / コネキシン43 / コネキシン45 / コネキシン40 / エナメル形成 / 卵胞
Research Abstract

Functional structures of gap junctions were analyzed by immunohistochemical, biochemical and molecular biological methods to examine the molecular diversity and distribution patterns of connexin protein family in various tissue and cell types as well as in different functional states.
1) During ameloblast development of the rat upper incisor, connexin 43 in undifferentiated cells once disappears just before enamel formation and reappears after maturation of secretion activity.
2) In granulosa cells of developing rat ovary, gap junctions are composed of Cx43 and Cx45 in various phosphorylation states. While Cx45 remains constant, Cx43 increases during maturations but disappears soon after ovulation. In addition, Cx40 detected in RNA blot in ovary is localized only in blood vessel wall.
3) In rat mammary glands, Cx26 and Cx32 are colocalized between secretory acinar cells and Cx43 is localized between myoepithels. They gradually increase during pregnancy. Cx32 and Cx43 are induced dramatica … More lly just after delivery, and reach a maximum at the early lactation period. There seems to bea gap between mRNA and protein expression levels as well as the additional shift from P1 to P2 phosphorylation state of Cx43.
4) In rat endocardium and blood vessels, endothelial cells can be classified as Cx40 dominant or Cx37 dominant type according to their connexin expression patterns.
5) In rat trachea Cx37 is expressed in smooth muscle cells after 3weeks after birth and increases thereafter and colocalized with Cx43, but not with Cx40.
6) In guinea pig, Cx43 is localized between fibroblast like cells in the outer layr of circular smooth muscles and deep muscular plexus of the small intestine and between submuscular plexus in the large intestine. These Cx43 containing cells are also c-Kit positive and interconnect the interstitial cells with intestinal pacemaker activity.
This research project has successfully elucidated the diverse aspects of gap junction functions in the light of composite protein connexin family with specific physiological properties ; Gap junction protein expressions are characteristically regulated during developmental stages, in different functional states and between different tissues. They are controlled by various mechanisms such as expression levels or phosphorylation states. Finally, specific connexin molecules are identified in specialized functional cells such as pacemaker cells in heart and intestinal movement. Less

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (61 results)

All Other

All Publications (61 results)

  • [Publications] K.Nakamura et al.: "Specific localization of gap junction protein,connexin45,in the deep mus-cular plexus of dog and rat small intestine." Cell Tissue Res.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Inai et al.: "Immunohistochemical localization of connexin43 in the enamel organ of the rat upper incisor during ameloblast development." Arch.Histol.Cytol.60. 297-306 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Fujimoto et al.: "Dynamics of connexins,E-cadherin and alpha-catenin on cell membranes during gap junction fromation." J.Cell Sci.110. 311-322 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Takihara et al.: "Tageted disruption of the mouse homologue of the drosophila polyhomeotic gene leads to altered anteroposterior patterniog and neural crest defects." Development. 124. 3673-3682 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Iida et al.: "Small GTP-binding protein,Rab6,is associated with secretory granules in atrial myoctes." Am.J.Physiol.272. C1594-C1601 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.M.Rahman et al.: "Expression and localization of annexin V and annexin VI during limb bud formation in the rat fetus." Anat.Embryol.195. 31-39 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] I.Yamanaka et al.: "Changes in the phosphorylation states of connexin43 in myoepitelial cells of lactating rat mammary glands." Eur.J.Cell Biol.72. 166-173 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Okuma et al.: "Colocalization of connexin 43 and connexin 45 but absence of connexin 40 in granulosa cell gap junctions of rat ovary." J.Reprod.Fertil.107. 255-264 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Iida et al.: "Identification of rab12 as a secretory granule-associated small GTP-binding protein in atrial myocytes." Circ.Res.78. 343-347 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] B.C.Shin et al.: "Immunolocalization of GLUT1 and connexin 26 in the rat placenta." Cell Tissue Res.285. 83-89 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] B.C.Shin et al.: "Connexin 43 and the glucose transporter,GLUT1,in the ciliary body of the rat." Histochem.Cell Biol.106. 209-214 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Iida: "Secretory pathway of atrial natriuretic peptide" Kaibogaku Zasshi. 70. 228-231 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Kojima et al.: "Induction and regulation of connexin26 by glucagon in primary cultures of adult rat hepatocytes." J.Cell Sci.108. 2771-2780 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Kuraoka et al.: "Characterization of isolated guinea pig liver gap junctions." Prog.Cell Res. 4. 111-114 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Manabe et al.: "Classification of ion channels in the luminal and abluminal membranes of guinea-pig endocardial endothelial cells." J.Physiol.484. 41-52 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Sekiguchi et al.: "Apoptosis is induced in BHK cells by the tsBN462/13 mutation in the CCG1/TAFII250 subunit of the TFIID basal transcription factor." Exp.Cell Res.218. 490-498 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Ito et al.: "Expression of tissue-type plasminogen activator and its inhibitor couples with development of capillary network by human microvascular endothelial cells on Matrigel." J.Cell.Physiol.162. 213-224 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Kamura et al.: "An abnormal fibrinogen Fukuoka II (Gly-B beta 15-->Cys) characterized by defective fibrin lateral association and mixed disulfide formation." J.Biol.Chem.270. 29392-29399 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Shibata et al.: "Gap junction formation and regulation in cultured adult rat and guinea pig cardiac muscle cell." Prog.Cell Res.4. 151-154 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] L.Wang et al.: "Annexin V is localized in association with Z-line of rat cardiac myocytes." Cardiovasc.Res.30. 363-371 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Yokoyama et al.: "A giant nucleopore protein that binds Ran/TC4." Nature. 376. 184-188 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Nakamura, A.Kuraoka, M.Kawabuchi, Y.Shibata.: "Specific localization of gap junction protein, connexin45, in the deep muscular plexus of dog and rat small intestine" Cell Tissue Res. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Inai, K.Nakamura, K.Kurisu, Y.Shibata.: "Immunohistochemical localization of connexin43 in the enamel organ of the rat upper incisor during ameloblast development" Arch.Histol.Cytol. 60. 297-306 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Fujimoto, A.Nagafuchi, S.Tsukita, A.Kuraoka, A.Ohokuma, Y.Shibata.: "Dynamics of connexins, E-cadherin and alpha-catenin on cell membranes during gap junction formation." J.Cell Sci. 110. 311-22 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Takihara, D.Tomotsune, M.Shirai, Y.Katohfukui, K.Nishii, M.A.Motaleb, M.Nomura, R.Tsuchiya, Y.Fujita, Y.Shibata, T.Higashinakagawa, K.Shimada.: "Targeted disruption of the mouse homologue of the drosophila polyhomeotic gene leads to altered anteroposterior patterning and neural crest defects." Development. 124. 3673-3682 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Iida, S.Tanaka, Y.Shibata.: "Small GTP-binding protein, Rab6, is associated with secretory granules in atrial myocytes" Am.J.Physiol. 272. C1594-601 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.M.Rahman, H.Iida, Y.Shibata: "Expression and localization of annexin V and annexin VI during limb bud formation in the rat fetus" Anat.Embryol. 195. 31-9 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] I.Yamanaka, A.Kuraoka, T.Inai, T.Ishibashi, Y.Shibata: "Changes in the phosphorylation states of connexin43 in myoepithelial cells of lactating rat mammary glands" Eur.J.Cell Biol. 72. 166-73 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Okuma, A.Kuraoka, H.Iida, T.Inai, K.Wasano, Y.Shibata: "Colocalization of connexin 43 and connexin 45 but absence of connexin 40 in granulosa cell gap junctions of rat ovary" J.Reprod.Fertil. 107. 255-64 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Iida, L.Wang, K.Nishii, A.Ookuma, Y.Shibata: "Identification of rab12 as a secretory granule-associated small GTP-binding protein in atrial myocytes" Circ.Res. 78. 343-7 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] B.C.Shin, T.Suzuki, T.Matsuzaki, S.Tanaka, A.Kuraoka, Y.Shibata, K.Takata: "Immunolocalization of GLUT1 and connexin 26 in the rat placenta" Cell Tissue Res. 285. 83-9 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] B.C.Shin, T.Suzuki, S.Tanaka, A.Kuraoka, Y.Shibata, K.Takata: "Connexin 43 and the glucose transporter, GLUT1, in the ciliary body of the rat" Histochem.Cell Biol. 106. 209-14 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Iida: "[Secretory pathway of atrial natriuretic peptide]" Kaibogaku Zasshi. 70. 228-31 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Kojima, T.Mitaka, Y.SHibata, Y.Mochizuki: "Induction and regulation of connexin26 by glucagon in primary cultures of adult rat hepatocytes" J.Cell Sci. 108. 2771-80 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A,Kuraoka, T.Inai, K.Nishii, I.Yamanaka: "S.Y.Characterization of isolated guinea pig liver gap junctions. (Eds. Y Kanno, Shimadzu, Y Shibata), Elsevier, Amsterdam" 4. 111-114 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Manabe, H.Ito, H.Matsuda, A.Noma, Y.Shibata.: "Classification of ion channels in the luminal and abluminal membranes of guinea-pig endocardial endothelial cells" J.Physiol. 484. 41-52 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Sekiguchi, T.Nakashima, T.Hayashida, A.kuraoka, S.Hashimoto, N.Tsuchida, Y.Shibata, T.Hunter, T.Nishimoto: "Apoptosis is induced in BHK cells by the tsBN462/13 mutation in the CCG1/TAFII250 subunit of the TFIID basal transcription factor" Exp.Cell Res.218. 490-8 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Ito, M.Ryuto, S.Ushiro, M.Ono, A.Sugenoya, A.Kuraoka, Y.Shibata, M.Kuwano: "Expression of tissue-type plasminogen activator and its inhibitor couples with development of capillary network by human microvascular endothelial cells on Matrigel." J.Cell.Physiol.162. 213-24 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Kamura, H.Tsuda, Y.Yae, S.Hattori, S.Ohga, Y.Shibata, S.Kawabata, N.Hamasaki.: "An abnormal fibrinogen Fukuoka II (Gly-B beta 15-->Cys) characterized by defective fibrin lateral association and mixed disulfide formation." J.Biol.Chem. 270. 29392-9 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Shibata, A.Miyahara, T.Okayama, A.Kuraoka, H.Iida: "Gap junction formation and regulation in cultured adult rat and guinea pig cardiac muscle cell. (Eds. Y Kanno, Shimadzu, Y Shibata)" Elsevier, Amsterdam. 4. 151-154 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] L.Wang, M.M.Rahmam, H.Iida, T.Inai, S.Kawabata, S.Iwanaga, Y.SHibata: "Annexin Vis localized in association with Z-line of rat cardiac myocytes" Cardiovasc.Res.30. 363-71 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Yokoyama, N.Hayashi, T.Seki, N.Pnte, T.Ohba, K.Nishii, K.Kuma, T.Hayashida, T.Miyata, U.Aebi, et al: "Agiant nucleopore protein that binds Ran/TC4" Nature. 376. 184-8 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Inai et al.: "Immunohistochemical localization of connexin43 in the enamel organ of the rat upper incisor during ameloblast development." Arch.Histol.Cytol.60. 297-306 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Fujimoto et al.: "Dynamics of connexins,E-cadherin and alpha-catenin on cell membranes during gap junction formation." J.Cell Sci.110. 311-322 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Takihara et al.: "Targeted disruption of the mouse homologue of the drosophila polyhomeotic gene leads to altered anteroposterior patterning and neural crest defects." Development. 124. 3673-3682 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Iida et al.: "Small GTP-binding protein,Rab6,is associated with secretory granules in atrial myocytes." Am.J.Physiol.272. C1594-C1601 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.M.Rahman et al.: "Expression and localization of annexin V and annexin VI during limb bud formation in the rat fetus." Anat.Embryol.195. 31-39 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] I.Yamanaka et al.: "Changes in the phosphorylation states of connexin43 in myoepithelial cells of lactating rat mammary glands." Eur.J.Cell Biol.72. 166-173 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] I.Yamanaka et al.: "Changes in the phosphorylation states of connexin43 in myoepithelial cells of lactating rat mammary glands." European Journal of Cell Biology. 72. 166-173 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] A.Okuma et al.: "Colocalization of connexin 43 and cnnexin 45 but absence of connexin 40 in granulosa cell gap junctions of rat ovary" Journal of Reproduction and Fertility. 107. 255-264 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.M.Rahman et al.: "Expression and localization of annexinV and annexinVI during limb bud formation in the rat fetus" Anatomy and Embryology. 195. 31-39 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] B.-C.Shin et al.: "Immunolocalization of GLUT1 and connexin26 in the rat placenta." Cell and Tissue Research. 285. 83-89 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] B.-C.Shin et al.: "Connexin43 and glucose transporter GLUT1 in the ciliary body of the rat." Histochemistry and Cell Biology. 106. 209-214 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Iida H and Y.Shibata: "Identification of rat12 as a secretory granule-associated small GTP-binding protein in atrial myocytes." Circulation Research. 78. 343-347 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 柴田洋三郎(分担): "解剖学者が語る人体の世界" 日本解剖学会編、風人社, 3 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y. Shibata et al: "Gap Junction formation and regulation in cultured adult rat and guinea pig cardiac muscle cells." Progress in Cell Research. 4. 151-154 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Bo-Chul Shin et al: "Immunolocalization of glutl and connexin26 in the rat placenta." Cell and Tissue Research. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] T. Kojima et al: "Induction and regulation of Cx26 by glucagon in primary culture of adult rat hepatocytes." Journal of Cell Science. 108. 2771-2780 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] L. Wang et al: "Annexin V is localized in association with Z line of rat cardiac myocytes." Cardiovascular Research. 30. 363-371 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K. Manabe et al: "Classification of ion chanels in luminal and abluminalmembrane of guinea pig endocardial endothelia." Journal of physiology. 484. 41-52 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] A. Kuraoka et al: "Characterization of isolated guinea pig liver gap junctions." Progress in Cell Research. 4. 111-114 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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