| Project/Area Number |
07407002
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Kyushu University |
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Principal Investigator |
AKAIKE Norio Kyushu University Fac.of Medicine, Professor, 医学部, 教授 (30040182)
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| Co-Investigator(Kenkyū-buntansha) |
HARATA Nobutoshi Kyushu University Fac.of Medicine, Assistant Professor, 医学部, 助手 (90264043)
NODA Mami Kyushu University Fac.of Medicine, Assistant Professor, 医学部, 助手 (80127985)
NABEKURA Junichi Kyushu University Fac.of Medicine, Associate Professor, 医学部, 助教授 (50237583)
尾野 恭一 九州大学, 医学部, 講師 (70185635)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥18,100,000 (Direct Cost: ¥18,100,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1995: ¥13,100,000 (Direct Cost: ¥13,100,000)
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| Keywords | lateral geniculate nucleus neuron / retrograde tracer DiI / excitatory amino acid response / inhibitory amino acid response / optic nerve denervation / neurotrauma / aging / intracellular chloride / シナプス・ブートン標本 / 自発性シナプス後電流 / 抑制性シナプス後電流 / GABA / 興奮性アミノ酸受容体 / オートレセプター / GABA_B受容体 / マグネシウムブロック |
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| Research Abstract |
In order to clarify the factors contributing to the activities of dorsal lateral geniculate nucleus (LGNd) neurons, we analyzed the physiological properties of excitatory and inhibitory amino acid receptors of the neurons freshly dissociated from animals with or without optic nerve denervation.
(1) The LGNd neurons identified by injection of a retrograde tracer, DiI,were prepared for the electrophysiological study using nystatin perforated patch recording mode under voltageclamp condition.
(2) The effect of excitatory amino acids on the neurons was stronger in the order of KA>NMDA>AMPA>Glu.
(3) A neurotransmitter candidate at retino-geniculate synapses, NAAG,mainly acted at NMDA receptors with a weak KA-like action on non-NMDA receptors.
(4) The values of relative permeability of Ca^<2+> for Cs^+ (Pc_a/Pc_S) were 9.57 and 0.16 for NMDA-and KA-induced responses, respectively.
(5) Intracellular C1^- concentration increased with aging : i. e. 35 mM for 0-2-day-old rat neuron, 22 mM for 2-week-old one, and 15 mM for 6-month-old one. Consequently, GABA depolarizes the neurons of immature rat.
(6) Denervation induced the rise of intracellular C1^- concentration and also decreased Mg^<2+>blockade of NMDA response.
(7) It was found in the study using 'synaptic bouton' preparation that NMDA,AMPA,KA receptors existed on GABAergic nerve endings. THese receptores facilitated tha GABA release from its nerve terminals. Both GABA_B and mGlu receptors also exist on GABA ergic nerve endigs and act as autoreceptors.
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