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Molecular studies of structures and functions of cytoskeleton and cell-cell junctions in blistering mechanisms for pemphigus an pemphigoid as a model system

Research Project

Project/Area Number 07407025
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionGIFU UNIVERSITY

Principal Investigator

KITAJIMA Yasuo  Gifu University, School of Medicine, Professor, 医学部, 教授 (70111797)

Co-Investigator(Kenkyū-buntansha) ESAKI Chikako  Gifu University, Univ.Hospital, Assistant, 医学部附属病院, 助手 (70283306)
AOYAMA Yumi  Gifu University, Univ.Hospital, Assistant, 医学部附属病院, 助手 (90291393)
SEISHIMA Mariko  Gifu University, Univ.Hospital, Lecturer, 医学部附属病院, 講師 (00171314)
井上 稲子  岐阜大学, 医学部附属病院, 助手 (60262771)
長田 和子  岐阜大学, 医学部附属病院, 助手 (30252133)
中谷 明美  岐阜大学, 医学部附属病院, 助手 (80242725)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥32,300,000 (Direct Cost: ¥32,300,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1995: ¥24,900,000 (Direct Cost: ¥24,900,000)
KeywordsKeratinocytes / Cell-cell junction / Cell-matrix junction / Desmosome / Hemidesmosome / Integrin / bullous diseases / Hyper keratosis / 細胞一基質間接着
Research Abstract

Epidermal keratinocytes response to the extracellular signals leading to control cell-cell and cell-matrix junctional adhesions, so as to control normal keratinization. The purposes of this research project are to elucidate molecular mechanisms for the control of structures and functions cytoskeletons and adhesion molecules in terms of signal transduction, by studying autoimmune blistering diseases, congenital epidermolysis bullosa and keratinization disorders as a model system. This approach may provide us with a mutual benefits between basic aspects of cytoskeleton and cell junctions and clinical aspects of bullous and keratinizing disorders.
Until last year, we had made a certain progress in clarifying the molecular mechanisms for blister formation in Pemphigus vulgaris (PV), which is an autoimmune blistering skin disease characterized by autoantibodies to a specific desmosomal constituent, desmoglein (Dsg) 3. We showed an involvement of calcium- and protein kinase C (PKC)-mediated i … More ntracellular signaling which leads to plasminogen activator (muPA) secretion and muPA receptor expression in response to pemphigus vulgaris (PV)-IgG-binding (J Invest Dermatol 104 : 33-37,1995,105 : 482-487,1997). This year, we showed that involvement of PV-PLC in PV-IgG-induced Biphasic increase in diacylglycerol, which is supposed to cause activation of PKC (J Invest Dermatol 109 : 650-655,1997), and that PV-IgG binding alone induced the phosphorylation of Dsg 3 and plakoglobin (PG). Although Dsg3 and PG were coprecipitated by PV-IgG-immunoprecipitation when treated with normal IgG,PG was not coprecipitated with Dsg3 when stimuated with PV-IgG,suggesting that PV-IgG-binding to Dsg causes the dissociation of Dsg from PG.We propose that these aberrant signal transduction responses to PV-IgG,which lead to Dsg3 and PG phosphorylation and their dissociation, may impair the desmosomal integrity from the inside of the cell, while PV-IgG-induced PKC signaling, linked with uPA secretion and its receptor expression on the cell surface, may mediate to dissociate preexisting desmosomes from the outside of the cell (J Clin Invest revised submitted). We also showed that 180kDa bullous pemphigoid antigen (BPAG2) is phosphorylated with TPA and this phosphorylation is always associated with translocation of BPAG2 (230 kDa) from cytosol to plasma membrane (J Invest Dermatol revised submitted). Less

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (40 results)

All Other

All Publications (40 results)

  • [Publications] Osada K, Kitajima Y, et al: "Pemphigus IgG activates and translocates protein kinase C from the cytosol to the particalate / cytoskeleton fractions in human keratinocytes" Journal of Investigative Dermatology. 108. 482-487 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Seishima M, Kitajima Y, et al: "Pemphigus IgG induces expression of urokinase plasminogen activator receptor on the cellsurface of cultured keratinocytes" Journal of Investigative Dermatology. 109. 650-655 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Satoh S, Kitajima Y, et al: "A vesicular variant case of bullous pemphigoid with autcantibodies against new basement membrane 20he 205-and 150-kDa proteins as well as BPAG2" British Journal of Dermatology. 137. 768-773 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kitajima Y, et al: "Internalization of the 180kDa bullous pemphigoid antigen asimmune complexes in basal keratinougtes : an important earlyeuent in blister formation in bullous pemhigoid" British Journal of Dermatology. 138. 71-76 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sato M, Kitajima Y, et al: "Precise ultrastructural localization of in vivo deposited IgG antibodies in fresh porilesional skin of patient with bullous pemphigoid" British Journal of Dermatology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hirako Y, Kitajima Y, et al: "Cleavage of BP180 (Type XVII collagen) yield a 120 kDa collagenous extracellular polypeptide" Journal of Biological Chemistry. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kitajima Yasuo: "Dermatology at the Millennium,Proceeclings of the 19th World Congress of Dermatology" Parthenon Publishing (in press),

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kitajima Yasuo: "Symposium on Skin : Interface of aliving system" Elsevier (in press),

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Osada K,Kitajima Y,et al: "Pemphigus IgG activates and translocates protein kinase C from the cytosol to the particulate/cytoskeleton fractions in human keratinocytes" Journal of Investigative Dermatology. 108. 482-487 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Seishima M,Kitajima Y.et al: "Pemphigus IgG induces expression of urokinase plasminogen activator receptor on the cell surface of cultured keratinocytes" Journal of Investigative Dermatology. 109. 650-655 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Satoh S,Kitajima Y,et al: "A vesicular variant case of bullous pemphigoid with autoantibodies against new basement membrane zone 205-and 150-kDa proteins as well as BPAG2" British Journal of Dermatology. 137. 768-773 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kitajima Y,et al: "Internalization of the 180kDa bullous pemphigoid antigen as immune complexes in basal keratinocytes : an important early event in blister formation in bullous pemphigoid" British Journal of Dermatology. 138. 71-76 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Satoh M,Kitajima Y,et al: "Precise ultrastructural localization of in vivo deposited IgG antibodies in fresh perilesional skin of patient with bullous pemphigoid" British Journal of Dermatology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hirako Y,Kitajima Y,et al: "Cleavage of BP180 (Type XVII collagen) yields a 120 kDa collagenous extracellular polypeptide" Journal of Biological Chemistry. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kitajima Yasuo: Dermatology at the Millennium, Proceedings of the 19th World Congress of Dermatology. Parthenon Publishing (in press),

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kitajima Yasuo: Symposium on Skin : Interface of a Living system. Elsevier (in press),

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Osada K, Kitajima Y, et al.: "Pemphigus IgG activates and translocates protein kinase C from the cytosol to the particalate/cytokeleton fractions in human keratinocytes" Journal of Investigative Dermatology. 108. 482-487 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Seishima M, Kitajima Y, et al.: "Pemphigus IgG induces expression of urokinase plasminogen activator receptor on the cell surface of cultured keratincytes" Journal of Investigative Dermatology. 109. 650-655 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Satoh S, Kitajima Y, et al.: "A vesicular variant case of bullous pemphigoid with auto antibodies against new basement membrane zone 205-and 150-kva proteins as well as BPAG 2" British Journal of Dermatology. 137. 768-773 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kitajima Y, et al.: "Internal ization of the 180kva bullous pemphigoid antigen as immune complexes in basal keratinocytes:an important early event in blistertormation in bullous pemphigoid" British Journal of Dermatology. 138. 71-76 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Satoh M, Kitajima Y, et al.: "Precise ultrastructural Iocalization of in vivo deposited IgG antibodies in fresh perilesional skin of patient with bullous pemphigoid" British Journal of Dermatology. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Hirako Y, Kitajima Y, et al.: "Cleavage of BP180(Type XVII collagen)yield a 120kDa collagenous extracellular polylesptide" Journal of Biological Chemistry. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Kitajima Yasuo: "Dermatology at the Millennium,Proceedings of the 19th world Congress of Dermatology" Parthenon Publishing (in press),

    • Related Report
      1997 Annual Research Report
  • [Publications] Kitajima Yasuo: "Symposium Skin;Interface of a Living system" Elsevier (in press),

    • Related Report
      1997 Annual Research Report
  • [Publications] Kitajima Y: "Adhesion molecules in the pathophysiology of bullous diseases" Europian Journal of Dermatology. 6. 399-405 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nojiri M,Kitajima Y,et al: "Immunofluorescence and immuroblat studies of herpetiform pemphigus" Europian Journal of Dermatology. 6. 406-408 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ban M,Kitajima Y,et al: "Kindler's syndrom with recurrence of bullae in the fifth decade" British Journal of Dermatology. 135. 503-504 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Seishima M,Kitajima Y,et al: "Expression of activin Air human keratirocytes at early stages of cultivation" FEBS Letters. 398. 120-124 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 北島康雄: "先天性表皮水疱症" 皮膚科の臨床. 38(1). 53-60 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Osada K,Kitajima Y,et al: "Pemphigus IgG activates and translocates protein kinase C from the cytosol to the particulate/cytoskeletor fractions in human keratinocytes" Journal of Investigative Dermatology. 108(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 北島康雄: "別冊・医学のあゆみ:接着分子の基礎と臨床" 医歯薬出版, 5 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 北島康雄: "New皮膚科学" 南江堂(印刷中), (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kitajima Y,et al: "A henidesnosomal transmexlxane cellogeous molecule the 180-k Dabullous pimphigoid artiger(BPA2),IS phaspharylated with 12-O-tetradecanoylpwboH3-acetate in a human squauous cell carcinoma cell line(DJM-1)" Epithelial Cell Biol:. in press.

    • Related Report
      1995 Annual Research Report
  • [Publications] Hashimoto T.,Kitajima Y.,et al: "Requlation of translocation of the desmoyokin/AHNAK protein to the plasma menbrau in kerationocytes by protein kinase C" Exp Cell Res. 217. 258-266 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kakoi H.,Kitajima Y.,et al: "Cytokeratin expression patterns by one-and-two dimertioral electrophoresis in pars flaccida cholesteatoma and parstersa cholesteatsma" Acta Oto-Laryngologica. 115. 804-810 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Seishima M.,Kitajima Y.,et al: "Penphigus IgG,bat rot bullous penphigaid IgG,causes atrarsiert increasein intracellular corcium and inositol 1,4,5-trisphosphate in DJM-cells,a squamous cell carcinoma line" J Invest Dermatol. 104. 33-37 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Esaki C,Kitajima Y.,et al: "Pharmacologic exiderce for imvolvement of phospholipase C in pemphigus IgG-inclmed irositosl 1,4,5-tnisphosphate gereration,intracellular calcium increease,and plasminoger activator secretion in DJM-1 cells,a squamous cellcarciramaline" J Invest Dermatol. 105. 329-333 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Aoyama Y.,Kitajima Y.,et al: "Involvement of protein kinase C in bradykinin-induced intracellnlar calcium increase in primary cultureat human Kerativacytes" J Dermatol Sci. 9. 111-116 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 北島康雄: "分子医科学シリーズ細胞・構造と機能" メジカルビュー社, 26 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 北島康雄: "今日の皮膚疾患治療指針:in press" 医学書院,

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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