| Project/Area Number |
07407040
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Shinshu University |
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Principal Investigator |
TAKAOKA Kunio Shinshu Univ., Dept. of Orthopaedic Surgery, Professor and Chairman, 医学部・整形外科学, 教授 (30112048)
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| Co-Investigator(Kenkyū-buntansha) |
OKUBO Kousaku Osaka Univ., Dept. of Molecular and Cellular Biology, Assistant Professor, 細胞工学センター, 助教授 (40233069)
YOSHIMURA Yasuo Shinshu Univ., Dept. of Orthopaedic Surgery, Assistant Professor, 医学部・附属病院整形外科, 助手 (10303439)
SHIMIZU Tominaga Shinshu Univ., Dept. of Orthopaedic Surgery, Assistant Professor, 医学部・整形外科学, 助手 (40283270)
橋本 淳 大阪大学, 医学部, 助手 (40237938)
谷川 浩隆 信州大学, 医学部, 助手 (40262711)
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| Project Period (FY) |
1995 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥18,100,000 (Direct Cost: ¥18,100,000)
Fiscal Year 1998: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1997: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1996: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1995: ¥8,000,000 (Direct Cost: ¥8,000,000)
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| Keywords | osteoblast / genes / proteins / bone formation / gene expression |
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| Research Abstract |
It is a clinically important issue to reveal it clear, what genes are expressed sequentially in osteoblastic process in osteoblastic cell line according to extrinsic and intrinsic signals. By comparing lists of 3'-directed partial cDNA sequences (gene signatures) randomly collected from various tissues, genes uniquely expressed individual tissue can be identified. A full length cDNA clone, corresponding to one such gene signature, unique to human osteoblast and adipose tissue, was isolated. This cDNA clone encodes a 845-amino acid protein that is almost identical to the mouse adipocyte transcription factor AEBP1 except that it has additional 105 amino acids in the N terminus. A northern hybridization showed that in the differentiating murine osteoblastic cell line, AEBP1 is also expressed but that it is shut off in the final calcification phase, suggesting a transcriptional repressive effect on genes for bone formation.
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