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Clinical and Experimental Studies on Growth and Metastasis of Human Osteosarcomas

Research Project

Project/Area Number 07407042
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionSapporo Medical university

Principal Investigator

ISHII Seiichi  Sapporo Medical university, Professor, 医学部, 教授 (20001000)

Project Period (FY) 1995 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥35,400,000 (Direct Cost: ¥35,400,000)
Fiscal Year 1998: ¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1997: ¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 1996: ¥11,600,000 (Direct Cost: ¥11,600,000)
Fiscal Year 1995: ¥12,000,000 (Direct Cost: ¥12,000,000)
Keywordsosteosarcoma / Erb B receptor family / ets oncogene family / MMP-9 / activation induced cell death / lymphokine activated killer cell / N-acetyl cysteine / lymphokine activated killer cell / F344ラット由来骨肉腫 / LM8マウス由来骨肉腫 / 接着分子導入細胞ワクチン / B-7ファミリー / 細胞外マトリックス分解酵素 / etsがん遺伝子ファミリー / SK-BR-3乳癌細胞株 / etsがん遺伝子 / C57BL / 6マウス線維肉腫 / ヒト胎児頭蓋由来骨芽細胞 / 接着分子 / ヒト骨内シアル酸含有蛋白 / 骨肉腫細胞株 / ヒートショック蛋白hsp90 / 抗腫瘍ワクチン / 分子生物学
Research Abstract

Research results are summarized as follows.
1. Establishment of an in vitro experimental system for evaluation of role of dexamethasone, extracellular matrix proteins, and TGF-beta in regulation of osteoblastic differentiation, using anewly developed human osteoblastic cell line, SV-HFO.
2. Detection of bone sialoprotein in bone-derived malignancies using newly developed monoclonal antibodies.
3. Experimental therapy of osteosarcoma with a combination of a chemotherapeutic reagent, MTX, and B7-transfected tumor vaccine in a rat orthotopic implantation model.
4. Demonstration of an inhibitory role of hsp9O in regulation of growth of a human osteosarcoma cell line, BOS.
5. Induction of MMP-9 and high metastatic phenotype in lines of human and mouse malignant tumor cells by transfection of ElAF, a member of eta oncogene fanily.
6. Demonstration of presence of concomitant tumor resistance, an inhibitory effect of the primary lesion on metastatic tumor growth, in a rat orthotopic tumor implantation model of osteosarcoma.
7. Demonstration of correlation between lack of erbB2 expression and poor prognosis in osteosarcoma patients.
8. Augmentation of anti-tumor immunity by inhibition of AICD, activation-induced cell death, of lymphokine-activated killer cells by pretreatmen with N-acetyl cysteine.

Report

(5 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (59 results)

All Other

All Publications (59 results)

  • [Publications] Iba,K.: "Transforming growth factor-β_1 downregulates dexamethasone-induced tetranectin gene expression during the in vitro mineralization of the human osteoblastic cell line SV-HFO" Federation of European Biomechanical Societies. 373. 1-4 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takada,J.: "Pretreatment with low doses of norethindrone potentiates the osteogenic effects of fluoride on human osteosarcoma cells" Journal of Bone and Mineral Research. 10. 1512-1522 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Iba,K.: "Extracellular matrix components induce differentiation of the human osteoblastic cell line SV-HFO in a soluble factor-dependent manner" Journal of Bone and Mineral Research. 14. 214-221 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Iba,K.: "Phase dependent effects of transforming growth factor β_1 on osteoblastic markers of human osteoblastic cell line SV-HFO during mineralization" Bone. 19. 363-369 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wada,T.: "A preliminary report of neoadjuvant chemotherapy NSH-7 study in osteosarcoma : a preoperative salvage chemotherapy based on clinical tumor response and the use of granulocyte colony-stimulating factor" Oncology. 53. 221-227 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takada,J.: "Dexamethasone enhances the osteogenic effects of fluoride in human TE85 osteosarcoma cells in vitro" Calcified Tissue International. 58. 355-361 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kaya,M.: "A single ets-related transcription factor,EIAF,confers invasive phenotype on human cancer cells" Oncogene. 12. 221-227 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Chiba,T.: "Fas-mediated apoptosis is modulated by intracellular glutathione in human T cells" Eur.J.Immunol.26. 1164-1169 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 早川 満: "頸椎原発骨肉腫の1剖検例" 整形外科. 47. 57-60 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 石川清一: "特集-悪性骨軟部腫瘍と機能再建術-悪性軟部腫瘍治療の進歩-" 関節外科. 15. 23-25 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 長森正史: "B7-1及びB7-2を導入したマウス骨肉腫細胞に対する抗腫瘍免疫の比較検討" 日整会誌. 71. S1368 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 常見健雄: "原発巣存在下の化学療法が肺転移結節形成に及ぼす影響" 日整会誌. 71. S1041 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hayakawa,M.: "B7-1-transfected tumor vaccine counteracts chemotherapy-induced immunosupression and prolongs the survival of rats bearing highly metastatic osteosarcoma cells" International Journal of Cancer. 71. 1091-1102 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hasegawa,T.: "Histological and immunohistochemical diversities, and proliferative activity and grading in osteosarcomas" Cancer Detection and Prevention. 21. 280-287 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nuka,S.: "All-trans retinoic acid inhibits dexamethasone-induced ALP activity and mineralization in human osteoblastic cell line SV HFO." Cell Struct.22. 27-32 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Watabe,T.: "The Ets-1 and Ets-2 transcription factors activate the promoters for invasion-associated urokinase and collagenase genes in response to epidermal growth factor." Internat.J.Cancer. 77. 128-137 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 山本英順: "N-acetylcysteineによる活性化末梢T細胞のアポトーシスの抑制とTNF産生の増強" 札幌医誌. 67. 89-97 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 鍋田裕樹: "癌ワクチン" 治療学. 32. 18-21 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 和田卓郎: "上腕骨近位部悪性骨肉腫瘍に対する患肢温存術-血管柄付き腓骨移植を用いた再建法-" 関節外科. 17. 148-153 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 薄井正道: "腫瘍 図説手の臨床" 石井清一編 メジカルビュー社,東京, 8 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Iba, K.: "Transforming growth factor-beta_1 downregulates dexamethasoneinduced tetranectin gene expression during the in vitro mineralization of the human osteoblastic cell line SV-HFO" Federation of European Biomechanical Societies. 373. 1-4 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takada, J.: "Pretreatment with low doses of norethindrone potentiates the osteogenic effects of fluoride on human osteosarcoma cells" Journal of Bone and Mineral Research. 10. 1512-1522 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Iba, K.: "Extracellular matrix components induce differentiation of the human osteoblastic cell line SV-HFO in a soluble factordependent manner" Journal of Bone and Mineral Research. 14. 214-221 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Iba, K.: "Phase dependent effects of transforming growth factor beta_1 on osteoblastic markers of human osteoblastic cell line SV-HFO during mineralization" Bone. 19. 363-369 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wada, T.: "A preliminary report of neoadjuvant chemotherapy NSH-7 study in osteosarcoma : a preoperative salvage chemotherapy based on clinical tumor response and the use of granulocyte colonystimulating factor" Oncology. 53. 221-227 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takada, J.: "Dexamethasone enhances the osteogenic effects of fluoride in human TE85 osteosarcoma cells in vitro" Calcified Tissue International. 58. 355-361 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kaya, M.: "A single ets-related transcription factor, EIAF, confers invasive phenotype on human cancer cells" Oncogene. 12. 221-227 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Chiba, T.: "Fas-mediated apoptosis is modulated by intracellular glutathione in human T cells" Eur.J.Immunol.26. 1164-1169 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hayakawa, M.: "B7-1 transfected tumor vaccine counteracts chemotherapyinduced immunosupression and prolongs the survival of rats bearing highly metastatic osteosarcoma cells" International Journal of Cancer. 71. 1091-1102 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hasegawa, T.: "Histological and immunohistochemical diversities, and proliferative activity and grading in osteosarcomas" Cancer Detection and Prevention. 21. 280-287 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nuka, S.: "All-trans retinoic acid inhibits dexamethasone-induced ALP activity and mineralization in human osteoblastic cell line SV HFO." Cell Struct.22. 27-32 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Watabe, T.: "The Ets-1 and Ets-2 transcription factors activate the promoters for invasion-associated urokinase and collagenase genes in response to epidermal growth factor." Internat.J.Cancer. 77. 128-137 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nuka,S.: "All-trans retinoic acid inhibits dexamethasone-induced ALP activity and mineralization in human osteoblastic cell line SV HFO." Cell Struct.22. 27-32 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Watabe,T.: "The Ets-1 and Ets-2 transcription factors activate the prmoters for invasion-associated urokinase and collagenase genes in responce to epidermal growth factor" Internat.J.Cancer. 77. 128-137 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 山本英順: "N-acetylcysteineによる活性化末梢T細胞のアポトーシスの抑制とTNF産生の増強" 札幌医誌. 67. 89-97 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 鍋田裕樹: "癌ワクチン" 治療学. 32. 89-97 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 和田卓郎: "上腕骨近位部悪性骨腫瘍に対する患肢温存術-血管柄付き腓骨移植を用いた再建法-" 関節外科. 17. 148-153 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 薄井正道: "腫瘍 図説手の臨床" 石井清一編 メジカルビュー社,東京, 8 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 長森正史: "B7-1及びB7-2を導入したマウス骨肉腫細胞に対する抗腫瘍免疫の比較検討" 日本整形外科学会雑誌. 71. S1368 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 常見健雄: "原発巣存在下の化学療法が肺転移結節形成に及ぼす影響" 日本整形外科学会雑誌. 71. S1041 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hayakawa M.: "B7-1-transfected tumor vaccine counteracts chemotherapy-induced immunosupression and prolongs the survival of rats bearing highly metastatic osteosarcoma cells." International Journal of Cancer. 71. 1091-1102 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hasegawa T.: "Histological and immunohistochemical diversities,and proliferative activity and grading in osteosarcomas." Cancer Detection and Prevention. 21. 280-287 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Watabe T.: "The Ets-1 and Ets-2 transcription factors activate the promoters for invasion-associated urokinase and collagenase genes in response to epidermal growth factor." International Journal of Cancer.

    • Related Report
      1997 Annual Research Report
  • [Publications] Kousuke Iba: "Transforming growth factor-β_1 downregulates dexamethasone-induced tetranectin gene expression during the in vitro mineralization of the human osteoblastic cell line.SV-HFO" Federation of European Biomechemical Societies. 373. 1-4 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Junichi Takada: "Pretreatment with low doses of norethindrone potentiates the osteogenic effects of fluoride on human osteosarcoma cells" Journal of Bone and Mineral Research. 10. 1512-1522 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kousuke Iba: "Extracellular matrix components induce differentiation of the human osteoblastic cell line SV-HFO in a soluble factor-dependent manner" Journal of Bone and Mineral Research. 14. 214-221 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kousuke Iba: "Phase-dependent effects of transforming growth factor β_1 on osteoblastic markers of human osteoblastic cell line SV-HFO during mineralization" Bone. 19. 363-369 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takuro Wada: "A preliminary report of neoadjuvant chemotherapy NSH-7 study in osteosarcoma : apreoperative salvage chemotherapy based on clinical tumor response and the use of granulocyte colony-stimulating factor" Oncology. 53. 221-227 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Junichi Takada: "Dexamethasone enhances the osteogenic effects of fluoride in human TE85 osteosarcoma cells in vitro" Calcified Tissue International. 58. 355-361 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mitsunori Kaya: "A single ets-related transcription factor,EIAF,confers invasive phenotype on human cancer cells" Oncogene. 12. 221-227 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takayuki Chiba: "Fs-mediated apoptosis is modulated by intracellular glutathione in human T cells" Eur.J.Immunol.26. 1164-1169 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 早川 満: "頸椎原発骨肉腫の1剖検例" 整形外科. 47. 57-60 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 石井清一: "特集-悪性骨軟部腫瘍と機能再建骨-悪性骨軟部腫瘍治療の進歩-" 関節外科. 15. 23-25 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 川上篤志: "90kDa 熱ショック蛋白質の細胞増殖における役割" 札幌医学雑誌. 63. 1-7 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fujisaki Toshihide: "In vitro chemosensitivity assay for human osteosarcoma using tumor xenografts" Clinical Orthopaedics. 313. 279-285 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Inoue Atsushi: "70-kDa heat shock cognate protein interacts directly with the N-terminal region of the retinoblastoma gene product pRb" J.Biological Chemistry. 270. 22571-22576 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Iba Kousuke: "Glucocorticoids induce mineralization coupled with bone protein expression without influence on growth of a human osteoblastic cell line" Cell Structure and Function. 20. 319-330 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kaya Mitsunori: "A single ets-related transcription factor,E1AF, confers invasive phenotype on human cancer cells." Oncogene. 12. 221-227 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 石井清一: "悪性骨軟部腫瘍治療の進歩" 関節外科. 15. 23-25 (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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