• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Expression and roles of growth factors in experimental myopic

Research Project

Project/Area Number 07407049
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

TOKORO Takashi  Tokyo Med & Dent.Uni, Medicine, Professor, 医学部, 教授 (20013865)

Co-Investigator(Kenkyū-buntansha) OHIDA Shin-ichiro  Tokyo Med & Dent.Uni, Dentistry, 歯学部, 助手 (10114745)
SHIMOKAWA M.toyatn  Tokyo Med & Dent.Uni, Dentistry, Ass.Prof., 歯学部, 助教授 (80014257)
SEKO Yuko  Tokyo Med & Dent.Uni, Medicine, 医学部, 助手
OHNO Kyoko  Tokyo Med & Dent.Uni, Medicine, Lecturer, 医学部, 講師 (30262174)
森田 博之  東京医科歯科大学, 医学部, 講師 (80230126)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1997: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1996: ¥3,200,000 (Direct Cost: ¥3,200,000)
KeywordsExperimental Myopia / Sclera / vetina / dopamine / growth factor / differentical display / mechanical strain / differential display / 青色光 / 視性刺激遮断 / 細膜 / 網膜色素上皮 / TGFβ / レチノイン酸
Research Abstract

(1) Unknown substances regulating ocular growth
Differential display cloning was conducted between the normal retina and the form-deprived retina. 46 bands were lsolated which were expressed more strongly in the normal retina than in the deprived retina. Following reamplification, dot blot differential hybridization and northern blot selected 3 cDNA clones. Sequence homology analysis revealed that those 3 cDNA clones have unknown sequences. Now, we are investigating about those 3 cDNA clones.
(2) Involvement of growth factors and retinoic acid
Immunohistochemical analyzes of experimental myopic chickens revealed that TGF-beta and IGF II are increased in the myopic sclera. Biochemical analyzes showed that TGF-beta is increased and bFGF is decreased in the myopic sclera and that TGF-beta is also increased in the myopic retina-chorold. RT-PCR-ELISA revealed that expression of retinoic acid receptor-beta is increased in the myopic sclera and HPLC analysis showed that retinoic acid is increase … More d in the myopic retina.
Tissue culture experiments showed that the retinal pigment epithelium (RPE) releases some growth regulating substances.
(3) Regulation of growth regulating mechanism of theRPE
-relationship to the blue light exposure-
Previous reports showed that visible light of short wave length "blue light" is toxic to the retina. But the mechanism of the toxicity has been unclear. We established an in virto model of exposure of blue light to the RPE and investigated the morphology of degeneration of the RPE by blue light in detail. We are investigating fuctional changes of the RPE by blue light exposure.
(4) Involvement of retinal dopamine in the roles of growth factors
Retinal dopamine has been reported to be decreased in the myopic eye. We demonstrated that apomorphine, dopamine agonist, inhibits growth stimulating effects of RPE on scleral cells in vitro. Now, we are investigating about roles of Muller cells. At first, we revealed that dopamine inhibits cell growth of Muller cells.
(5) Involvement of mechanical strain in the roles of growth factors
Ocular hypertension and accommodation have been reported to play a role in the ocular enlargement. We investigated the effects of mechanical strain on the biochemical changes of the scleral cells and the RPE cells in vitro. Mechanical strain affected cell growth of scleral cells and the release of growth factors from the RPE cells. Less

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Seko Y.et al.: "Expression of BFGF and TGF-Bete-2 in experimental myopia in chieks" Investigative Ophthalmology υ Visual Science. 36(3). 1183-1187 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Seko Y.et al.: "Influance of bFGF as a potent growth stiundatorand TGFβ as a growth regulafor on scleral cgebdcicttcs of suleral F.latblcuts" Ophthalanic Fes. 27(3). 144-152 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Seko Y.et al.: "In vivo and intotro asocmition of vetinoic acid with form-depbibation mayoma in the chiclc" Exp.Eys Res. 63. 443-452 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Seko Y.et al.: "Apocmplgire imgibits growth stlmulating effects on vitinell pigout epithelium in vitro" Cell Biuchem-Liumct,. 15(3). 191-196 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kuakari T.et al.: "Regional scleral chamges in form-dipvioationmyopior in checks" Exp.Eys Res. 64. 465-476 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Seko Y.et al.: "Retinoic acidincreases in theretina of the chick with form-dipvivation myoria" Ophthalmic Res. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 世古 裕子: "眼科診療Q&A 近視発生の病態" 六法出版著(in press), (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Seko Y.et al: "Expression of bFGF and TGFbeta_2 in experimental myopic in chicks" Inoest.Ophthalmol.Uis.Sci. 36(6). 1183-1187 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Seko Y.et al: "Influence of bFGF as a potent growth stimulator and TGF beta as a growth regulator on scleral chondrocytes and scleral fibroblasts in vitro" Ophthalmic Res. 27(3). 144-152 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Seko Y.et al: "In v co and in vitro association of refinoic acid with form-dipriration anyolna in the chick" Exp.Eye Res. 63. 443-452 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshino Y.et al: "Visual cliprioation myopia with translucent and black goggles" Ophthalmologia. 211. 4-7 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Seko Y.et al: "Apomorphine inhibits growth stimulating effects on retinal pigment epithelium in vitro" Cell Biochem Funct. 15(3). 191-196 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kusakari T.et al: "Regional scleral changes in form-dipviration myopia in chicks" Exp. Eye. Res. 64. 465-476 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Seko Y.et al: "Retinoic acid increases in the vetina ot the chick with form-cliprivation myopia" Ophthalnic Res. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kusakari T. et al: "Regional scleral changes in form deprivation myopia in chicks" Exp.Eye.Res.64. 465-476 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Pang.JT. et al: "Observation of ultrastructwal change in culturel retinal pigaitep theliuor following exposure to blue light" Graefes Arch.Clin.Exp.Ophthalmal.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Seko Y. et al: "Retinoic acid increnses in the retina of the thick with form diprivition onyopia" Ophthalmic Res.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 世古裕子: "眼科診療Q&A 近視発生の病態" 六法出版者(in press), (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yoshino Y.et al.: "visual deprivation myopia with translucent and black goggles" Ophthalmologica. 211. 4-7 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Seko Y.et al.: "In vivo and in vitro association of retinoic acid with form-deprivation my cyia in chick" Experimental Eye Research. 63. 443-452 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Seko Y.et al.: "Apomorphine inhibits growth-stimulaling effects of volinal pigment epithelium in vitro" Cell Biochemistry and Function. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 草刈匡世: "眼科臨床プラクティス22.「やさしい眼の生化学・分子生物学」強膜の組成と成長因子" 文光堂, 4 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yuko Seko: "Influence of bFGF as a potent growth stimulator and TGF-β as a growth regulator on scleral chondrocytes and scleral fibroblasts in vitro" Ophthalmic Res.27. 144-152 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yuko Seko: "Expression of TGF-β2 and bFGF in experimental myopia in chicks" Invest.Ophthalmol.Vis.Sci.36(6). 1183-1187 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hiroyuki Morita: "A clinical study of the development of posterior vitreous detachment in high myopia" Retina. 15(2). 117-124 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kyoko Matsui-Ohno: "The progression of lacquer cracks" Retina. (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] 山下和雄: "ヒヨコ強度近視眼における強膜伸展性の検討" 視覚の科学. 16(2). 79-83 (1995)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1996-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi