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Host defense mechanism in human periodontitis

Research Project

Project/Area Number 07407054
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionNiigata University

Principal Investigator

HARA Kohji  Niigata University School of Dentistry, Professor, 歯学部, 教授 (20018419)

Co-Investigator(Kenkyū-buntansha) TAI Hideaki  Niigata University Dental Hospital, Associate, 歯学部・附属病院, 助手 (30272826)
KOBAYASHI Tetsuo  Niigata University School of Dentistry, Associate, 歯学部, 助手 (00215344)
青柳 昌子  新潟大学, 歯学部・附属病院, 助手 (40251839)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥29,500,000 (Direct Cost: ¥29,500,000)
Fiscal Year 1997: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1995: ¥20,700,000 (Direct Cost: ¥20,700,000)
KeywordsPeriodontitis / Gingival Crericular Fluid / RT-PCR / in situ hybridization / complement Receptor / TIMP / 接着分子 / コラゲナーゼ インヒビター / T細胞レセプター
Research Abstract

I.In this study we attempted to determine the mRNA levels of complement receptor type 1 and 3 (CR1, CR3) on PMNs in GCF by using reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization (ISH). Both CR1 and CR3 mRNA levels relative to b-actin were significantly lower in GCF-PMNs than in PB-PMNs (GCF-CR1 : 32.75 (]SY.+-。[) 22.93% PB-CR1 : 65.30 (]SY.+-。[) 43.25% p<0.005, GCF-CR3 : 9.09 (]SY.+-。[) 5.34% PB-CR3 : 30.14 (]SY.+-。[) 18.80% p<0.005). In ISH,a greater majority of PB-PMNs showed positive CR1 and CR3 mRNA expressions, while only a few PMNs showed positive signals in GCF.Our data in this study suggests that increased expressions of CR on the PMN cell surface appear to be unrelated to de novo synthesis.
DNA binding activity of NF-kB and AP-1 was examined in neutrophils stimulated with LPS purified from P.gingivalis, a major pathogenic bacteria of periodontitis lesion. Porphyromonas gingivalis LPS enhanced the activity reaching a Peak at a concentration of 5 … More 00ng/ml in the absence of serum. The NF-kB activation stimulated with 10ng/ml of P.gingivalis LPS was approximately 44% suppressed by treatment of neutrophils with anti-CD14 antibody under the presence of serum. Increase in the steady-state IL-8 mRNA level was concomitantly observed by stimulation of neutrophils with 500ng/ml of P.gingivalis LPS under the absence of serum. These results indicate that P-gingivalis LPS activates NF-kB and AP-1 both in serum-dependent and -independent manners, followed by increased IL-8 transcription in neutrophils, and suggesting a role for P.gingivalis LPS in IL-8 synthesis by neutrophils in inflamed gingiva and GCF.
II.This study presents the exact cell types and localization of tissue inhibitors of metalloproteinases (TIMPs) production sites in periodontal diseased gingiva by means of in situ hybridization. Gingival tissue specimens were fixed, embedded and hybridized in situ with specific digoxigenin-labeled cRNA probes (386 and 496 bp). TIMP-1 and -2 mRNAs were expressed on macrophages, mononuclear cells, capillary endothelial cells and some fibroblasts throughout the gingival tissue. In periodontitis, TIMP-1 and -2 mRNA-expressing cells showed significantly different localization. TIMP-1 mRNA was broadly observed in the gingival connective tissue while TIMP-2 mRNA was predominantly expressed in the connective tissue adjacent to the pocket epithelium (P<0.01). Fewer TIMPs mRNA were observed in minimal gingivitis than in periodontitis, especially in the middle zone of gingival tissue. Thus, TIMP-1 and TIMP-2 mRNA was detected differentially and site-specifically in periodontal diseased gingival tissue. Less

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] 杉田 典子: "Characterization of Complement Receptor Type1 and Typ3 mRNA Expression on Plymorpno nuclear Lencocyte (Neutrophils) in Gigival Crericular Fluid From Pericdontitis-aftected Patient" Arch oral Biology. 42. 113-120 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 久保田 健彦: "In sifu hybridization study on tissue inhibitors of metallopratenase (TIMP) mRNA-expressing cells in numar inflamed gingival fissue" Journal of Periodontal Research. 32. 467-472 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 杉田 典子: "Activation of trans cription Factors and IL-8 Expression in Nentrophils Stimulated with hipopoly saccharide from porphyromonas gingiralis" Inflammation. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Sugita, Y.Matsuki, H.Yoshie and K.Hara.: "Characterization of Complement Receptor Type 1 and Type 3 mRNA Expression on Polymorphonuclear Leucocytes (Neutrophils) in Gingival Crevicular Fluid from Periodontitis-affected Patients" Archs.oral Biol.vol.42. 113-120 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Kubota, Y.Matsuki, T.Nomura and K.Hara: "In situ hybridization study on tissue inhibitors of metalloproteinase (TIMP) mRNA-expressing cells in human inflamed gingival tissue." Journal of Periodontal Research. vol.32. 467-472 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Sugita, A.Kimura, Y.Matsuki, T.Yamamoto and K.Hara: "Activation of transcription Factors and IL-8 Expression in Neutrophils Stimulated with Lipopoysaccharide from porphyromonas gingivalis." Inflammation. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 杉田 典子: "charaterization of Complement Receptor Type 1 and Type 3 mRNA Expression on poly morphonuclear Leucucyte (Neutroplils) in Gimgioal Crericulor Fluid from Perioclontitis affected Ptient" Arch oral Biology. 42. 113-120 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 久保田 健彦: "In situ hybridization study on tissue inhibitors, of metalloprotenose (TIMP) mRNA - expressing cells in human inflamed gingiral tissue" Journal of Periodontal Research. 32. 467-472 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 杉田 典子: "Actiration of transcription Factors and IL-8 Expression in Neutrophils Sfimulated with Lippolysacharide from porphy romonas gingivalis" Inflammation. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 杉田典子: "Charaterization of CR1 and CR3 mRNA Expressions on Polymorphonuclear Leukocytes in Gingival Crericular Fluid from Periodonfitis-affected Patients" Archs oral Biology. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 宮崎朗: "Loss of Fcr roceptor and impaired phagucytosis polymorphonuclear leukocytes in gingival crevicular fluid" Journal of Periodontal Research. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 久保田健彦: "In situ hybridization study on fissue inhibitors of Metalloproteinase (TIMPs) mRNA-expressing cells in human inflamed gingival fissue" Journal of Periodontal Research. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 久保田健彦: "Expression of mRNA for Matrix Metalloproteinase and Tissue Inhibitors of Metalloproteinase in Periodontitis-Affocted Human Gingival Tissue" Archs oral Biology. (予定). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 中島貴子: "Biased T cell receptor Vgene usage in tissues with periodontal disease" Journal of Periodontal Research. 31. 2-10 (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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