| Project/Area Number |
07407056
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
AMAGASA Teruo Tokyo Medical and Dental University, Dentistry, Professor, 歯学部, 教授 (00014332)
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| Co-Investigator(Kenkyū-buntansha) |
FUJII Eiji Tokyo Medical and Dental University, Dentistry, Assistant, 歯学部, 助手 (20221541)
IWAKI Hiroshi Tokyo Medical and Dental University, Dentistry, Lecturer, 歯学部, 講師 (70107308)
TSUCHIDA Nobuo Tokyo Medical and Dental University, Dentistry, Professor, 歯学部, 教授 (60089951)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥20,100,000 (Direct Cost: ¥20,100,000)
Fiscal Year 1997: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1996: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥16,700,000 (Direct Cost: ¥16,700,000)
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| Keywords | Comparative Genomic Hybridization(CGH) / Oral (Squamous Cell) Carcinoma / Chromosome / DNA Copy Number / Tumor Malignancy / Lymph Node Metastasis / Comparative Genomic Hybridization (CGH) / Comparative Genomic / Hybridization (CGH) |
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| Research Abstract |
1. To find and map chromosomal regions with amplifications and deletions in oral cancsers by CGH,we performed CGH on 17 oral squamous cell carcinomas cell lines and compared to those obtained by Southern hybridization. Recurrent loci for amplification were observed on 8q22-26,3q25-27,7p12,11q13,13q33,14q, 15q and 20q12. Deletions were seen on 3p, 8p, 5q21-22,7q31 and 18q21. There was a strong concordance for amplification detected by the CGH and Southern hybridization methods.
2. When the original tumors and the cell lines were compared, their profiles were essestialy similar with one exception. Further, there was no region that commonly changed in the cell lines, but not in the original tumors, suggesting that the DNA copy number changes observed in the cell lines mostly represent those of the original tumors.
3. We correlated clinical and histopathological results to tomor DNA copy number changes. The increases on chromosomes 6p23-25 and 8q24 were specificaly observed in the groups of WHO Grade II and Grade III,whereas the deletions on chromosomes 11p11-13 and 18q21 observed in the same groups. Comparison of DNA copy number changes to TNM classification indicated that the deletions on chromosomes 1p36 and 10q25-26 might be related to tumor progression.
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