| Project/Area Number |
07408018
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Nagoya University |
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Principal Investigator |
HATTA Ichiro Nagoya University Department of Applied Physics Professor, 工学研究科, 教授 (70016070)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Hiroshi Nagoya University Department of Applied Physics Assistant Professor, 工学研究科, 助手 (80236314)
HIBINO Masahiro Nagoya University Department of Applied Physics Assistant Professor, 工学研究科, 助手 (00242834)
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| Project Period (FY) |
1995 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥27,700,000 (Direct Cost: ¥27,700,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1996: ¥13,400,000 (Direct Cost: ¥13,400,000)
Fiscal Year 1995: ¥11,900,000 (Direct Cost: ¥11,900,000)
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| Keywords | biomembrane / phospholipid / phase transition / X-ray diffraction / calorimetry / interaction / molecular recongnition / interdigitated structure / 分子認識 / インターディジテイテッド構造 |
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| Research Abstract |
Microscopic structure and thermodynamic approach is quite important to investigate biological functioning on biomembrane surface. From this point of view, we developed a method to perform simultaneous X-ray diffraction and differential scanning calorimetry measurements. We applied this method to studies on aqueous dispersions of phospholipid and phospholipid/phosphatidylglycerol. In the former, we obtained the results that the X-ray diffraction intensity is proportional to the transition enthalpy. In the latter, we found the existence of H_<II> structure that plays an important role in biological functioning. As examples for interaction between a phospholipid molecule and a small molecule, we studied systems of phospholipid/alcohol and phospholipid/sugar. In the former, we analyzed the interdigitated structure and proposed a micro-structural model. In the latter, depending on sugars the condition that stabilizes liquid-crystalline phase was revealed. Furthermore, as an example of interaction between a phospholipid molecule and a small molecule, we studied a system of phospholipid/water by thermal analysis and X-ray diffraction. From this study, we found the number of water molecule incorporated in the interbilayer. Furthermore, we studied the structure of a monolayer of fatty acids formed on a graphite surface by scanning tunneling microscopy with atomic resolution and this method offers possibility to carry out a study on interaction at atomic scale.
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