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Molecular recognition mechanism on biomembrane surface in primary biological functioning

Research Project

Project/Area Number 07408018
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionNagoya University

Principal Investigator

HATTA Ichiro  Nagoya University Department of Applied Physics Professor, 工学研究科, 教授 (70016070)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Hiroshi  Nagoya University Department of Applied Physics Assistant Professor, 工学研究科, 助手 (80236314)
HIBINO Masahiro  Nagoya University Department of Applied Physics Assistant Professor, 工学研究科, 助手 (00242834)
Project Period (FY) 1995 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥27,700,000 (Direct Cost: ¥27,700,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1996: ¥13,400,000 (Direct Cost: ¥13,400,000)
Fiscal Year 1995: ¥11,900,000 (Direct Cost: ¥11,900,000)
Keywordsbiomembrane / phospholipid / phase transition / X-ray diffraction / calorimetry / interaction / molecular recongnition / interdigitated structure / 分子認識 / インターディジテイテッド構造
Research Abstract

Microscopic structure and thermodynamic approach is quite important to investigate biological functioning on biomembrane surface. From this point of view, we developed a method to perform simultaneous X-ray diffraction and differential scanning calorimetry measurements. We applied this method to studies on aqueous dispersions of phospholipid and phospholipid/phosphatidylglycerol. In the former, we obtained the results that the X-ray diffraction intensity is proportional to the transition enthalpy. In the latter, we found the existence of H_<II> structure that plays an important role in biological functioning. As examples for interaction between a phospholipid molecule and a small molecule, we studied systems of phospholipid/alcohol and phospholipid/sugar. In the former, we analyzed the interdigitated structure and proposed a micro-structural model. In the latter, depending on sugars the condition that stabilizes liquid-crystalline phase was revealed. Furthermore, as an example of interaction between a phospholipid molecule and a small molecule, we studied a system of phospholipid/water by thermal analysis and X-ray diffraction. From this study, we found the number of water molecule incorporated in the interbilayer. Furthermore, we studied the structure of a monolayer of fatty acids formed on a graphite surface by scanning tunneling microscopy with atomic resolution and this method offers possibility to carry out a study on interaction at atomic scale.

Report

(5 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (38 results)

All Other

All Publications (38 results)

  • [Publications] K.Tsuchida: "^2H NMR Study on Two Motional States of Acyl Chains in Pβ' Phase of Phospholid" J.Phys.Soc.Jpn.67・4. 1473-1476 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] J.F.Nagle: "Comment on“Growth of Molecular Superlattice in Fully Hydrated Dipalmitoylphosphatidylcholine during Subgel Phase Formation Process"" Eur.Phys.J.B1. 399-400 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Adachi: "Phase Transition in a Phosphatidylcholine Membrane Induced by Halogenated Alcohol" J.Phys.Soc.Jpn.67・9. 3147-3150 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Hibino: "Microscopic Origin of the Odd-Even Effects in Monolayer of Fatty Acids Formed on a Graphite Surface by Scanning Tunneling Microscopy" J.Phys.Chem.B. 102・23. 4544-4547 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] I.Hatta: "Diagnosis of phase shift in a temperature-modulated calorimetric method" J.Thermal Analysis. 54. 577-584 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Tsuchida: "^2H NMR Study on Two Motional States of Acyl Chains in Pbeta' Phase of Phospholid" J.Phys.Soc.Jpn.67-4. 1473-1476 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] J.F.Nagle: "Comment on "Growth of Molecular Superlattice in Fully Hydrated Dipalmitoylphosphatidylcholine during Subgel Phase Formation Process"" Eur.Phys.J.B1. 399-400 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Adachi: "Phase Transition in a Phosphatidylcholine Membrane Induced by Halogenated Alcohol" J.Phys.Soc.Jpn.67-9. 3147-3150 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Hibino: "Microscopic Origin of the Odd-Even Effects in Monolayr of Fatty Acids Formed on a Graphite Surface by Scanning Tunneling Microscopy" J.Phys.Chem.B.102-23. 4544-4547 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] I.Hatta: "Diagnosis of phase shift in a temporature-modulated calorimetric method" J.Thermal Analysis. 54. 577-584 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Tsuchida: "^2H NMR Study on Two Motional States of Acyl Chains in Pβ' Phase of Phospholid" J.Phys.Soc.Jpn.67・4. 1473-1476 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] J.F.Nagle: "Comment on “Growth of Molecular Superlattice in Fully Hydrated Dipalmitoylphosphatidylcholine during Subgel Phase Formation Process"" Eur.Phys.J.B1. 399-400 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Adachi: "Phase Transition in a Phosphatidylcholine Membrane Induced by Halogenated Alcohol" J.Phys.Soc.Jpn.67・9. 3147-3150 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Hibino: "Microscopic Origin of the Odd-Even Effects in Monolayer of Fatty Acids Formed on a Graphite Surface by Scanning Tunneling Microscopy" J.Phys.Chem.B.102・23. 4544-4547 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] I.Hatta: "Diagnosis of phase shift in a temperature-modulated calorimetric method" J.Thermal Analysis. 54. 577-584 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] G.Nechev: "Defects in Langmuir-Blodgett Film of Cadmium Arachidate Detected with Atomic Force Microscopy" Jpn.J.Appl.Phys.36・[5A]. L580-L582 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] C.Wolf: "Phase transition sequence between fluid liquid-crystalline and interdigitated lamellar gel phases in mixed-chain diacyl phsphatidylcholine" Chem.Phys.Lipids. 87. 111-124 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Takahashi: "On exothermic transformation from metastable gel phase to stable crystalline phase of fully hydrated dimyristoylphosphatidylethanolamine in heating scan" Chem.Phys.Lipids. 89. 83-89 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Kodama: "Interlamellar waters in dimyristoylphosphatidylethanolamine-water system as studied by calorimetry and X-ray diffraction" Biochim.Biophys.Acta. 1329. 61-73 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Takahashi: "Behavior of ice-water transition in dimyristoyl-phosphatidylethanolmine-water system" Thermochimica Acta. 303. 93-99 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Takahashi: "Trehalose-Induced Destabilization of Interdigitated Gel Phase in Dihexadecylphosphatydylcholine" Biophys.J.73. 3030-3038 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Takahashi: "Cubic Phases in Hybrated 1:1 and 1:2 Dipalymitoyl-phosphatidylcholine-Dipalmitoylglycerol Mixtures" Biophys.J.70・3. 1407-1411 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Takahashi: "Effects of cholesterol on the lamellar and the inverted hexagonal phases of dielaidoylphosphatidylethanolamine" Biochim.Biophys.Acta. 1289. 209-216 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] I.Hatta: "AC calorimetric aspect of dynamic differential scanning calorimetry" Thermochim.Acta. 272. 49-52 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Hibino: "Molecular motion at domain boundaries in fatty acid monolayers on graphite observed by scanning tunneling microscopy" Thin Solid Films. 273. 272-278 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] I.Hatta: "High Precision Heat Capacity Measurement by Dynamic Differential Scanning Calorimetry" Jpn.J.Appl.Phys.35・7A. L858-L860 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Hibino: "Scanning tunnelling microscopy study on dynamic structural formation in mixed fatty-acid monolayers at liquid/graphite interface" Thin Solid Films. 281-282. 594-597 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] I.Hatta: "Simultapeous differential scanning calorimetry and time-resolved x-ray diffraction of lipid-water system. I.Relationship between chain melting and endothermic heat at the main transition of a dipalmitoylphosphatidylcholine-water system" Chem.Phys.Lipids. 76. 115-121 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] I.Hatta: "Phase transitions of quartz studied by AC calorimetry" Thermochimica Acta. 245. 301-308 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] P.J.Quinn: "Characterization of Complexes Formed in Fully Hydrated Dispersions of Dipalmitoyl Derivatives of Phosphatidylcholine and Diacylglycerol" Biophysical Journal. 68. 1374-1382 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Adachi: "Interdigitated Structure of Phospholipid-Alcohol Systems Studied by X-Ray Diffraction" Biopysical Journal. 68. 1850-1855 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 八田一郎: "脂質膜と低分子およびポリペプチドの相互作用" 生物物理. 35. 29-31 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 八田一郎: "ダイナミックDSCから見た相転移現象の研究" 熱測定. 22. 92-94 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Adachi: "Cholesterol effects on ripple structure studied by small-angle neutron and X-ray diffraction" Phisica B. 213&214. 760-762 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Hibino: "Atomic images of saturated and unsaturated fatty acids at the Liquid/graphite interface and the difference of tunneling currents between them observed by scanning tunneling microscopy" Jpn.J.Appl.Phys.34. 610-614 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Hibino: "Molecular Arrangements of Fatty Acids and Cholesterol at Liquid/Graphite Interface Observed by Scanning Tunneling Microscopy" Jpn.J.Appl.Phys.34. 3354-3359 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Hibino: "Ordered Structure Form by Biologically Related Molecules" Jpn.J.Appl.Phys.34. 3930-3936 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Takahashi: "Structural and Thermotropic Properties of Calcium-Dimyristoylphosphatidic Acid Complexes at Acidic and Neutral pH Conditions" Biophysical Journal. 69. 1464-1472 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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