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Molecular Mechanisms of Stopping and Starting the Cell Cycle

Research Project

Project/Area Number 07408022
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionTokyo Institute of Technology

Principal Investigator

KISHIMOTO Takeo  Fac.Bioscience & Biotechnology, Tokyo Institute of Technology, Professor, 生命理工学部, 教授 (00124222)

Co-Investigator(Kenkyū-buntansha) OKUMURA Eiichi  JSPS Postdoctoral Fellow, 生命理工学部, 学振特別研究員
OHSUMI Keita  Fac.Bioscience & Biotechnology, Tokyo Institute of Technology, Assist.Prof., 生命理工学部, 助手 (20221822)
TACHIBANA Kazunori  Fac.Bioscience & Biotechnology, Tokyo Institute of Technology, Assist.Prof., 生命理工学部, 助手 (60212031)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥24,900,000 (Direct Cost: ¥24,900,000)
Fiscal Year 1997: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 1996: ¥7,100,000 (Direct Cost: ¥7,100,000)
Fiscal Year 1995: ¥13,500,000 (Direct Cost: ¥13,500,000)
Keywordscell cycle control / meiosis / fertilization / cdc2 kinase / Cdc25 phosphatase / Weel kinase / Mos / MAP kinase / 細胞周期 / 減数分裂周期 / G2 / M / G1 / S移行 / 核外移出 / Junキナーゼ / M期開始 / S期抑制 / cdc25フォスファターゼ / S期開始
Research Abstract

Meiotic cell cycle in oocytes is characterized by the arrest and release at G2-phase of immature oocytes, lack of S-phase in interkinesis period and the second arrest after the completion of meiosis. To elucidate the molecular mechanisms underlying these characteristics, we have found the followings in the project.
1. G2-phase arrest and its release : G2-phase arrest in immature starfish oocytes is supported by Weel-like kinase, but not Weel itself. At the release, both phosphorylation of Cdc25 phosphatase and suppression of Weel-like kinase occur independently of cdc2 kinase activity, resulting in the initial activation of cdc2 kinase.
2. Lack of S-phase in interkinesis period : We have developed from Xenopus oocyte extracts a cell-free system, "meiotic extracts", which mimics meiotic M/M transition. Successful N/N transition depends on both Mos, whose downstream is known MAP kinase and unknow pathway, and low protein levels of Weel.
3. GI-phase arrest in mature eggs : In mature starfish eggs, MAP kinase activity is necessary and sufficient for the G1-phase arrest and suppression of S-phase. As an upstream of MAP kinase, we have isolated starfish Mos for the first time in invertebrate oocytes which do not arrest at the second meiotic metaphase. Comparison among Xenopus, mouse and starfish oocytes reveals that a conserved role of Mos might be to ensure the successful entry into meiosis II, thus preventing parthenogenetic activation.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (42 results)

All Other

All Publications (42 results)

  • [Publications] Hisanaga,S.,et al.: "Porcine brain neurofilament-H tail domain kinases..." Cell Motil.Cytoskel.31. 283-297 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Okumura,E.,et al.: "Initial triggering of M-phase in starfish..." J.Cell Biol.132. 125-135 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kishimoto,T.: "Starfish maturation-promoting factor." Trends Biochem.Sci.21. 35-37 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tachibana,K.,et al.: "MAP kinase links the fertilization signal..." EMBO J.16. 4333-4339 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Okano-Uchida,T.,et al.: "In vivo Regulation of cyclin A/Cdc2 and..." Develop.Biol.197. 39-53 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kishimoto,T.: "Cell cycle arrest and release in starfish oocytes..." Semin.Cell Develop.Biol.9. 549-557 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 岸本健雄(分担): "細胞機能と代謝マップII. 細胞の動的機能" 東京化学同人(日本生化学会編), 292 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kishimoto,T.(分担): "Encyclopedia of Reproduction,Vol.3" Academic Press,San Diego(Eds.E.Knoloil or J.D.Neill), 850 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hisanaga, S., Uchiyama, M., Hosoi, T., Yamada, K., Honma, N., Ishiguro, K., Uchida, T., Dahl, D., Ohsumi, K.and Kishimoto, T.Porcine brain neurofilament-H tail domain kinases: "its idetification as CDK5/p26 complex, and comparison with CDC2/cyclin B kinase." Cell Motil.Cytoskel.31. 283-297 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Okumura, E., Sekiai, T., Hisanaga, S., Tachibana, K.and Kishimoto, T.: "Initial triggering of M-phase in starfish oocytes : A possible novel component of MPF besides cdc2 kinase." J.Cell Biol.132. 125-135 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kishimoto, T.: "Starfish maturation-promoting facctor. Trends Biochem." Sci.21. 35-37 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tachibana, K., Machida, T., Nomura, Y.and Kishimoto, T.: "MAP kinase links the fertilization signal transduction pathway to the G1/S-phase transition in starfish eggs." EMBO J.16. 4333-4339 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ookata, K., Hisanaga, S., Sugita, M., Okuyama, A., Murofushi, H., Kitazawa, H., Chari, S., Bulinski, J.C.and Kishimoto, T.: "MAP4 is the in vivo substrate for cdc2 kinase in HeLa cells---Identification of an M-phase specific and a cell cycle-independent phosphorylation site in MAP4---." Biochemistry. 36. 15873-15883 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kishimoto, T.and Okumura, E.: "In vivo Regulation of the entry into M-phase : initial activation and unclear translocation of cyclin B/Cdc2. in Progress in Cell Cycle Res. (Meijer, L., Guidet, S.and Philippe, M.eds.)" Plenum Press, New York. vol.3. 241-249 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Okano-Uchida, T., Sekiai, T., Lee, K., Okumura, E., Tachibana, K.and Kishimoto, T.: "In vivo Regulation of cyclin A/Cdc2 and cyclin B/Cdc2 through meiotic and early cleavage cycles in starfish." Develop.Biol.197. 39-53 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shimada, A., Ohsumi, K.and Kishimoto, T.: "An indirect role for cyclin B-Cdc2 in inducing chromosome condensation in Xenopus egg extracts." Biol.Cell.90. 519-530 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kishimoto, T.: "Cell cycle arrest and release in starfish oocytes and eggs." Semin.Cell Develop.Biol.9. 549-557 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kishimoto, T.: "Oocyte maturation and spawning in starfish." in Encyclopedia of Reproduction (Knobil, E.and Neill, J.D.eds.). vol.3. 481-488 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Tachibana et al.: "MAP kinase links the fertilization signal....." EMBO J.16. 4333-4339 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] N.Monma et al.: "Phosphorylation of retinobl'astoma protein at....." Neurosci.Lett.235. 45-48 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Itoh et al.: "Phosphorylation states of microtubule-associated....." Biochemistry. 36. 12574-12582 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Ookata et al.: "MPA4 is the in vivo substrate for cdc2 kinase...." Biochemistry. 36. 15873-15883 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Okano et al.: "In vivo regulation of cyclin A/Cdc2 and....." Develop.Biol.(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 永井由貴子・岸本健雄: "サイクリン分解の制御機構" 蛋白質・核酸・酵素. 42. 2225-2232 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Kishimoto and E.: "Progress in Cell Cycle Researce,vol.3" Okumura(分担)Plenum Press(New York), 241-299 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 岸本健雄(分担): "細胞機能と代謝マップII(日本生化学会編)" 東京化学同人 (印刷中), (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kishimoto,T.: "Starfish maturation-promoting factor" Trends Biochem.Sci.21. 35-37 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Okumura,E.,et al.: "Initial triggering of M-phase in starfish…" J.Cell Biol.132. 125-135 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Uchida,K.,et al.: "Isolation and characterization of the cDNA…" Plant Cell Physiol.37. 825-832 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Honma,N.,et al.: "Two types of apoptotic cell death of rat…" J.Neurochem.67. 1856-1865 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tachibana,K.,et al.: "MAP Kinase links the fertilization signal…" EMBO J.(印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 岸本健雄: "Cdc2キナーゼはどのようにしてM期を実現するのか" 蛋白質・核酸・酵素. 41. 1839-1846 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 岸本健雄: "英語論文セミナー「現代の細胞生物学」(分担)" 講談社, 168 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 岸本健雄: "生物学辞典 第4版(分担)" 岩波書店, 2027 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ookata,k.,et al.: "Cyclin B interaction with microtupule-associated...." J.Cell Biol.128. 849-862 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ohsumi,K.,et al.: "Dependence of removal of sperm-specific proteins...." Develop.Growth Differ.37. 329-336 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hisanaga,S.,et al.: "Porcine brain neurofilament-H tail domain Kinase:.." Cell Motil.Cytoskel.31. 283-297 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tsurumi,C.,et al.: "Degradation of c-Fos by the 26S proteasome is....." Mol.Cell.Biol.15. 5682-5637 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Okumura,E.,et al.: "Initial triggering of M-phase in starfish oocytes:..." J.Cell Biol.132. 125-135 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kishimoto,T.: "Starfish maturation-pronoting factor." Trends Biochem.Sci.21. 35-37 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 岸本健雄・山下茂: "細胞周期の分子生物学" (監訳)メディカル・サイエンス・インターナショナル, 248 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 岸本健雄(分担): "バイオサイエンス用語ライブラリー「細胞周期」" 羊土社, 166 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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