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Moleculer mechanisms of cell differentiation and morphogenesis during early animal development

Research Project

Project/Area Number 07408023
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionThe University of Tokyo

Principal Investigator

ASASHIMA Makoto  The University of Tokyo, Graduate School of Arts and Sciences, Professor, 大学院・総合文化研究科, 教授 (00090564)

Co-Investigator(Kenkyū-buntansha) FUKUI Akimasa  The University of Tokyo, Graduate School of Arts and Sciences, Research assistan, 大学院・総合文化研究科, 助手 (80262103)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥35,600,000 (Direct Cost: ¥35,600,000)
Fiscal Year 1997: ¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1996: ¥10,300,000 (Direct Cost: ¥10,300,000)
Fiscal Year 1995: ¥17,900,000 (Direct Cost: ¥17,900,000)
Keywordsneurogenesis / head structune / kidney formation / activin / organogenesis / vitellogenin / cell soating out / embriyonic induction / 卵形成 / 腎形成 / XIRB / 初期発生 / 血球分化 / 神経誘導 / ツメガエル / ビテロゲニン受容体 / 形成体 / 内胚葉分化 / 胴尾部構造 / 神経形成遺伝子 / 植物極化遺伝子
Research Abstract

We got many important results about the morphogenesis and cell differentiation using the amphibian embryos. (1) After the treatment of activin A on animal caps, we made subtraction methods to clone the neurogenesis-related genes. And we cloned and analyzed the new genes such as Xran, XHMG-2, XIRF-6, XFRP,XGS,XNLRR-1. (2) In vitro system, we succeeded to make the head structure and trunk-tail structures. These structures were made with sandwith methods, in which the activin-treated ectoderm was combined. Depend on the pre-culture time, the induced structures were different. (3) Some kinds of genes which were related with pronephros (kidney) formation were cloned and analyzed. These genes were Na-K-ATPase alpha-subunit, CIRP,XFKBP,and the expression patterns of these genes are examined. (4) Incorporations of activin, follistatin and vitellogenin were examined using the labeling of these substance with ^<125>I and gold colloid particles. (5) Treatment with high concentration of activin to animal cap induced the beating heart, and expressed many kinds of endodermal marker genes. (6) Vitellogenin receptor during oogenesis was also cloned and analyzed. Above these data were very important to understand the cell differentiation and morphogenesis at the molecular level during animal development.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (38 results)

All Other

All Publications (38 results)

  • [Publications] Kinoshita, K.et al.: "Effect of activin and lithium onisolated Xenopus animal blastomeres andresponse alteration at the midblastula transition" Development. 121. 1581-1589 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ariizumi, T., et al: "Haed and trunk-tail organizing effects of the gastrula ectoderm of Cynops pyrrhogaster after treatment with activin A" Roux' Archives Develop.Biol.204. 427-435 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Okabayashi, K., et al.: "cDNA Cloning and Expression of the leavis Vitellogenin receptor" B.B.R.C.224. 406-413 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ariizumi, T., et al.: "Activin treated urodele ectoderm:A model experomental system for cardiogenesis" Int.J.Develop.Biol.40. 715-718 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takabayasi, K., et al.: "Conversion of ectderm into a neural fate by ATH-3,a ventabrate basic helix-loop-helix gene homologous to Drosophil proneural gene atonal." EMBO Journal. 16. 384-395 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nishinakamura, R., et al: "Xenopus FK 506-Binding Protein Homolog Induces a Secondary Axis in Frog Embryos,Which Is Inhibited by CoexistingBMP 4 Signaling" Biochem.Biophys.Res.Commun.239. 585-591 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 浅島 誠: "図解 生物科学講座(3)「発生生物学」" 朝倉書店, 182 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Asashima et.al.: "Taniguchi Symposium on Developmental Biology IX" SEIKO Inc.ed.by T.S.Okada, 101 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kinoshita, K.et al.: "Effect of activin and lithium on isolated Xenopus animal blastomeres and response alteration at the midblastula transition" Development. 121. 1581-1589 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ariizumi, T., et al.: "Head and trunk-tail organizing effects of the gastrula ectoderm of Cynops pyrrhogaster after treatment with activin A" Roux'Archives Develop.Biol.204. 427-435 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Okabayashi, K., et al.: "cDNA Cloning and Expression of the leavis Vitellogenin receptor" B.B.R.C.224. 406-413 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ariizumi, T., et al.: "Activin treated urodele ectoderm : A model experomental system for cardiogenesis" Int.J.Develop.Biol.40. 715-718 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takabayashi, K., et al.: "Conversion of ectoderm into a neural fate by ATH-3, a vertebrate basic helix-loop-helix gene homologous to Drosophila proneural gene" The EMBO journal. 16 (2). 384-395 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nishinakamura, R., et al.: "Xenopus FK 506-Binding Protein Homolog Induces a Secondary Axis in Frog Embryos, Which Is Inhibited by Coexisting BMP 4 Signaling" B.B.R.C.239. 585-591 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Asashima: Biological Science Series (3) 「Developmental Biology」. Asakura-shoten, (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Asashima et.al.: Taniguchi Symposium on Developmental Biology IX. SEIKO Inc.ed.by T.S.Okada, 101 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hatada,S., et al.: "A novel gene encoding a ferredoxin reductase-ralated protein expressed in the neuroetderm in Xenopus neurula." Gene. 194/2. 297-299 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tonegawa,A., et al.: "Mesodermal subdivision along the mediolateral axis inchicken controlled by different concentrations of BMP-4." Development. 124. 1975-1984 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takakura,N., et al.: "PDGFRa expression during mouse embryogenesis : Immunolocalization analyzed by whole mount immunostaining using the monoclonal anti-mouse-PDGFRa antibody APA5." J.Hist.Chem.Cytochem.45. 883-892 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tamura,K., et al.: "Retinoic acid changes the proximodistal developmental competence and affinity of distal cells in the developing chick limb bud." Developmental Biology. 188. 224-234 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ohuchi,H., et al.: "The mesenchymal factor,FGF10,initiates and maintains the outgrowth of thechick limb bud through interaction with FGF8,an apical ectodermal factor" Development. 124(11). 2235-2244 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Okabe,M.et al.: "Two-step induction of chordotonal organ precursors in Drosophila embryogenesis." Development. 124. 1045-1053 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Asashima et.al.: "Taniguchi Symposium on Developmental Biology IX" SEIKO Inc.ed.by T.S.Okada, 101 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Okano,H.et al.: "The regulatory mechanisms of neural development : roles of cell-autonomous and non cell-autonomous cues on cell fate decisions" Frontiews of Neural Development Springer-Verlag, 168 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Okabayashi,K.,et al.: "cDNA Cloning and Expression of the Xenopus leavis Vitellogenin receptor" B.B.R.C.224. 406-413 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Uochi,T.,et al.: "Sequential Gene Expression during pronephric Tubule Formation in vitro in Xenopus Ectoderm" Develop.Growth Differ.38(6). 625-634 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ariizumi,T.,et al.: "Activin treated urodele ectoderm:A model experomental system for cardiogenesis" Int.Develop.Biol.40. 715-718 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tiedemann,H.,et al.: "Determination,induction and pattern formation in early amphibian embryos" Develop.Growth Differ.38. 233-246 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Uchiyama,H.,et al.: "Occurrence of Immunoreactive Activin/Inhibin β B in Gonadotrophs,Thyrotrophs,and Somatotrophs of the Xenopus Pituitary" General and Comparative Endocrinology. 102. 1-10 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takabayashi,K.,et al.: "Conversion of ectderm into a neural fate by ATH-3,a ventabrate basic helix-loop-helix gene homologous to Drosophila proneural gene atonal" EMBO Journal. 16. (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 浅島 誠: "図解 生物科学講座(3)「発生生物学」" 朝倉書店, 182 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 浅島 誠 他: "英語論文セミナー「現代の発生生物学」" 講談社サイエンティフック, 168 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kinoshita,K.,Asashima,M.: "Effect of activin and lithium on isolated Xenopus animal blastomeres and response alteration at the midblastula transition" Development. 121. 1581-1589 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yokota,C.,Mukasa,T.,Higashi,M.,Odaka,A.,Muroya,K.,Uchiyama,H.,Elo,Y.,Asashima,M.,Momoi,T.: "Activin Induces the Expression of the Xenopus Homologue of Sonic Hedgehog dunig Mesoderm Formation in Xenopus Explants" B.B.R.C.207. 1-7 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nakano,H.,Uchiyama,H.,Fukui,A.,Sugino,H.Asashima,M.: "Inhibin /Activin β A-subunit monomer has Xenopus mesoderm-inducing activity" Hormone Research. 44. 15-22 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yamagishi,T.,Nishimatsu,S.,Nomura,S.,Asahima,M.,Murakami,K.,Ueno,N.: "Expression of BMP-2,4 Genes during Early Development in Xenopus" Zoological Science. 12. 355-358 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sekiguchi,K.,Yokota,C.,Kaname,T.,Q-Wen-Fan,Kadomatsu,K.,Asashima,M.,Muramatsu,T.: "Restricted expression of Xenopus midkine gene during early development" J.Biochemistry. 118. 94-100 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ariizumi,T.,Asashima,M.after treatment with activin A: "Haed and trunk-tail organizing effects of the gastrula ectoderm of Cynops pyrrhogaster" Roux′Archives Develop.Biol.204. 427-435 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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