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Design of Temperature-Responsive Surfaces and Its Application for Aqueous Chromatography

Research Project

Project/Area Number 07408030
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionTokyo Women's Medical College

Principal Investigator

SAKURAI Yasuhisa  Tokyo Women's Medical College, Institute of Biomedical Enginering, Professor, 医学部, 教授 (20010027)

Co-Investigator(Kenkyū-buntansha) KIKUCHI Akihiko  Tokyo Women's Medical College, Institute of Biomedical Enginering, Research Assi, 医学部, 助手 (40266820)
SUZUKI Ken  Tokyo Women's Medical College, Institute of Biomedical Enginering, Research Assi, 医学部, 助手 (90216375)
OKANO Teruo  Tokyo Women's Medical College, Institute of Biomedical Enginering, Professor, 医学部, 教授 (00130237)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥27,300,000 (Direct Cost: ¥27,300,000)
Fiscal Year 1996: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1995: ¥23,700,000 (Direct Cost: ¥23,700,000)
KeywordsThermo-responsive surface / Aqueous chromatography / Poly (N-isopropylacrylamide) / Hydrophilic / Hydrophobic / Hydrophobic interaction / Steroid hormones / Polypeptides / クロマトグラフィー / 親・疎水性
Research Abstract

This project aimed to develop a novel aqueous chromatography system in which the polarity of stationary phases is controlled by external temperature changes to separate and purify solutes without changing their structure and functions. This system is quite different from reversed phase chromatography in which the polarity of stationary phases is altered by the addition of organic solvents in water with various mixing ration. In the proposed system, the surface of the aminated silica stationary phase was modified with temperature-responsive poly (N-isopropylacrylamide) (PIPAAm) with a carboxyl end. The successful modification of the surfaces with polymers were confirmed by means of titration of residual surface functional groups, X-ray photoelectron spectroscopy, and atmic force microscope. These surfaces showed temperature-responsive wettability changes, being hydrophilic at lower temperature below polymer transition temperature, and become hydrophobic above the transition temperature. … More The elution profiles of five steroids were then investigated by changing temperature using high-performance liquid chromatography column packed with PIPAAm-grafted silica using aqueous mobile phase. Retention times of hydrophobic steroids increased with increasing temperature without any change in the eluent composition. Retarded elution time of hydrophobic steroids resulted from the stronger interaction of these compounds with the PIPAAm-grafted surfaces in which the surface property becomes hydrophobic with temperature increase. As a consequence, gradient elution-like effect is achieved with a single mobile phase by controlling external temperature. We further investigated the possibility of this temperature-responsive chromatography for polypeptide separation. Polypeptides with similar number of amino acid residues were successfully separated at higher temperature where hydrophobic interaction of solutes with stationary phases are primarily influenced the separation efficacy. In conclusion, elution of target substances is controlled only by a small change in column temperature without any further modification of the aqueous mobile phase in the proposed chromatography. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] 薬師寺大二: "温度応答性高分子をグラフトした表面を用いた新しい温度制御型タンパク質クロマトグラフィー" 治療と診断のシステム 化学工学シンポジウムシリーズ55 化学工学会治療システム研究会編. 67-70 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Kanazawa: "Analysis of peptides and proteins by temperature-responsive chromatographic system using N-isopropylacrylamide polymer-modified columns" J. Pharmaceu. Biomed. Anal.(印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Kanazawa: "Temperature-responsive liquid chromatography. 2.Effects of hydrophobic groups in Nisopropylacrylamide copolymer-modified silica" Anal.Chem.69(印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Okano,N.Yamada,M Okuhara,H.Sakai,Y.Sakurai: "Mechanism of cell detachment from temperature-modulated hydrophilic-hydrophobic polymer surfaces" Biomaterials. 16. 297-303 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.G.Takei,T.Aoki,K.Sanui,N.Ogata,Y.Sakurai,T.Okano: "Temperature-modulated platelet and lymphocyte interactions with poly (N-isopropylacrylamide)-grafted surfaces" Biomaterials. 16. 667-673 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Okano,A.Kikuchi,Y.Sakurai,Y.Takei,N.Ogata: "Temperature-responsive poly (N-isopropylacrylamide) as a modulator for alteration of hydrophilic/hydrophobic surface properties to control activation/inactivation of platelets" Journal of Controlled Release. 36. 125-133 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Kanazawa,K.Yamamoto,Y.Matsushima,N.Yakai,A.Kikuchi,Y.Sakurai,T.Okano: "Temperature-responsive chromatography using poly (N-isopropylacrylamide)-modified silica" Analytical Chemistry. 68. 100-105 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hideko Kanazawa, Kazuo Yamamoto, Yoshikazu Matsushima, Nobuharu Takai, Akihiko Kikuchi, Yasuhisa Sakurai, Teruo Okano: "Temperature-responsive chromatography using poly (N-isopropylacrylamide) -modified silica" Analytical Chemistry. 68. 100-105 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Taiji Yakushiji, Kiyotaka Sakai, Akihiko Kikuchi, Takao Aoyagi, Yasuhisa Sakurai, Teruo Okano: "A novel chromatography using temperature-responsive polymer grafted ssurface for protein separation" System for Medicine and Diagnosis, Research Group of Remedy, Japanese Society for Chemical Engineering. 67-70 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hideko Kanazawa, Yuki Kashiwase, Kazuo Yamamoto, Yoshikazu Matsushima, Nobuharu Takai, Akihiko Kikuchi, Yasuhisa Sakurai, Teruo Okano: "Analysis of peptides and proteins by temperature-responsive chromatographic system using N-isopropylacrylamide polymer-modified columns" Journal of Pharmaceutical and Biomedical Analysis. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hideko Kanazawa, Yuki Kashiwase, Kazuo Yamamoto, Yoshikazu Matsushima, Akihiko Kikuchi, Yasuhisa Sakurai, Teruo Okano: "Temperature-responsive liquid chromatography. 2. Effects of hydrophobic groups in N-isopropylacrylamide copolymer-modified silica" Analytical Chemistry. 69, (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Kanazawa: "Temperature-responsive chromatography using poly(N-isopropylacrylamide)-modified silica" Anal.Chem.68. 100-105 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 薬師寺大二: "温度応答性高分子をグラフトした表面を用いた新しい温度制御型タンパク質クロマトグラフィー" 治療と診断のシステム 化学工学シンポジウムシリーズ55 化学工学会治療システム研究会編. 67-70 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Kanazawa: "Analysis of peptides and proteins temperature-responsive chromatographic system using N-isopropylacrylamide polymer-modified columns" J.Pharamaceu.Biomed.Anal.(印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Kanazawa: "Temperature-responsive liquid chromatography. 2.Effects of hydrophobic groups in N-isopropylacrylamide copolymer-modified silica" Anal.Chem.69(印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] T. Okano: "Temperature-responsive poly(N-is opropylacrylamide) as a modulator for alteration of hydrophilic/hydrophobic surface properties to control activation/inactivation of platelets" Journal of Controlled Release. 36. 125-133 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] H. Kanazawa: "Temperature-responsive chromatography using poly(N-is opropylacrylamide)-modified silica" Analytical Chemistry. 68. 100-105 (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2021-09-15  

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