| Project/Area Number |
07454208
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
遺伝
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
HARADA Tomoko National Institute of Genetics, Department of Population Genetics, Professor, 集団遺伝研究系, 教授 (80000256)
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| Co-Investigator(Kenkyū-buntansha) |
TAJIMA Fumio University of Tokyo, Graduate School of Science, Professor, 大学院・理学系研究科, 教授 (30183065)
TAKANO Toshiyuki National Institute of Genetics, Department of Population Genetics, Research Asso, 集団遺伝研究系, 助手 (90202150)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥7,300,000 (Direct Cost: ¥7,300,000)
Fiscal Year 1996: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1995: ¥3,800,000 (Direct Cost: ¥3,800,000)
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| Keywords | DNA polymorphism / Molecular evolution / Slightly deleterious mutation / Adaptive evolution |
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| Research Abstract |
Molecular mechanism of gene evolution has been a fundamental problem in evolutionary biology for many years. One focus of controversy concerns the effect and significance of natural selection. The present study mainly put a focus on dissection of DNA changes into nonsynonymous and synonymous substitutions which occurred among closely related species in mammals and Drosophila. Amino-acid-altering substitution, or nonsynonymous substitution, is naturally considered to be more exposed to natural selection than synonymous change. Thus, the comparison between these two patterens in a gene provides a good opportunity to evaluate the effect of natural selection. Along this line, we studied the pattern of molecular evolution in mammalian and Drosophila genes. Particularly, we could reveal that the ratio of replacement to synonymous changes in the lineage leading to rodent is lower than that in the primate lineage. This is consistent with the nearly neutral mutation model, which advocates that most of amino acid changes are slightly deleterious. The variation in nonsynonymous substitution among lineages was significant in mammals after weighting the lineage effects. The ratio of replacement to synonymous substitutions also varied significantly among lineages for some of the genes studied in Drosophila. This episodic molecular evolution cannot be explained by the epistatic effect among amino acid sites. Thus, fluctuation of population size or selective constraint should be taken into consideration. Otherwise, positive Darwinian evolution may have some impact on molecular evolution. Further study on higher-order interaction will be also needed to understand the molecular evolution of the complicated genetic systems and to dissect the effects of natural selection and random genetic drift.
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