Molecular Genetical Study of the Process of Transition to Stationary Phase in Yeast

• Project/Area Number: 07454217
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 植物生理
• Research Institution: (National Institute for Basic Biology) Okazaki National Research Institutes (1996); The University of Tokyo (1995)
• Principal Investigator: OHSUMI Yoshinori Division of Bioenergetics, Department of Cell Biology, National Institute for Basic Biology Professor, 基礎生物学研究所, 教授 (30114416)
• Project Period (FY): 1995 – 1996
• Project Status: Completed (Fiscal Year 1996)
• Budget Amount: ¥2,900,000 (Direct Cost: ¥2,900,000); Fiscal Year 1996: ¥2,900,000 (Direct Cost: ¥2,900,000)
• Keywords: Stationary Phase / Nutrient / Starvation / Autophagy / Yeast / APG genes / apg変異

Research Abstract

It is not known the mechanism to enter stationary phase at a molecular level. We have developed a system for monitoring autophagy by prcessing of a cytosolic from of alkaline phosphatase in the vacuole. Using it we detected induction of autophagy before reaching stationary phase. We also found that under various starvation conditions yeast cells induce bulk protein degradation by autophagy. Induction of autophagy provides a good marker to analyze the process of transition to stationary phase. During these two years, we have cloned and sequenced most of the APG genes essential for induction of autophagy. APGI encodes a novel protein kinase, but we do not know the substrates or regulation of its activity yet. We found apg13 mutation is suppressed by overexpression of APGI.Apg13p is a novel hydrophilic protein. Interestingly immunoblot analysis revealed that Apg13 is heavily phosphorylated during vegetative growth, but dephoshorylated under starvation. Other APG genes are all nobel. Chacterization of these APG gene products is now undergoing.

Research Products

• [Publications] Shirahama,K et al: "Vacuolar function in the phosphate homeostasis of the yeast Saccharomyces cerevisiae." Plant Cell Physiol. 37. 1090-1093 (1996)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Scott,S.V.et al: "Cytoplasm-to-vacuole targeting and autophagy employ the same machinery to deliver proteins to the yeast vacuole." Proc.Natl.Acad.Sci.USA. 93. 12304-12308 (1996)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kametaka,S.et al: "Structual and functional analyses of APG5,a gene involved in autophagy in yeast." Gene. 178. 139-143 (1996)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nakamura,N.et al: "Acidification of vacuoles is required for autophagic degradation in the yeast,Saccharomyces cerevisiae." J. Biochem.193 (in press). (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Funakoshi,T.et al: "Analyses of APG13 gene involved in the autophagy in yeast,Saccharomyces cerevisiae." Gene. 179 (in press). (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Matsuura,A.et al: "Apglp,a novel protein kinase required for the autophagic process in Saccharomyces cerevisiae." Gene. 179 (in press). (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 大隅良典: "生化学" 日本生化学会, 6 (1996)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Wada, Y., Ohsumi, Y., Kawai, E., and Ohsumi, M.: "Mutational analysis of Vam4/Ypt7p function in the vacuolar biogenesis and morphogenesis in the yeast, Saccharomyces cerevisiae." Protoplsma. 191. 126-135 (1996)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Shirahama, K., Yazaki, Y., Sakano, K Wada, Y.and Ohsumi, Y.: "Vacuolar function in the phosphate homeostasis of the yeast Saccharomyces cerevisiae" Plant Cell Physiol.37. 1090-1093 (1996)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Scott, S., H-Gravink, A.Morano, K,Noda, T.and Ohsumi, Y., and Klionsky, D.: "Cytoplasm-to vacuole targeting and autophagy employ the same machinery to deliver proteins to the yeast vacuole." Proc.Natl.Acad, Sci.93. 120304-12308 (1996)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kametaka, S., Matsuura, A., Wada, Y,and Ohsumi, Y.: "Structual and functional analyzes of APG5, a gene involved in autophagy in yeast." Gene. 178. 139-143 (1996)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nakamura, N., Matsuura, A., Wada, Y., and Ohsumi, Y.: "Acidification of vacuoles is required for autophagic degradation in the yeast, Saccharomyces cerevisiae." J.Biochem.121. 338-344 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Funakoshi, T., Matsuura, A., Noda, T., and Ohsumi, Y.: "Analyzes of APG13 gene involved in the autophagy in yeast, Saccaromyces cerevisiae." Gene. 179(in press). (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Matsuura, A., Wada, Y., and Ohsumi, Y: "Apglp, a novel protein kinase required for the autophagic process in Saccharomyces cerevisiae" Gene. 179(in press). (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Shirahama,K et al,: "Vacuolar function in the phosphate homeostasis of the yeast Saccharomyces cerevisiae." Plant Cell Physiol. 37. 1090-1093 (1996)
• [Publications] Scott,S.V.et al,: "Cytoplasm-to-vacuole targeting and autophagy employ the same machinery to deliver proteins to the yeast vacuole." Proc.Natl.Acad.Sci.USA. 93. 12304-12308 (1996)
• [Publications] Kametaka,S.et al,: "Structual and functional analyses of APG5,a gene involved in autophagy in yeast." Gene. 178. 139-143 (1996)
• [Publications] Nakamura,N.et al,: "Acidification of vacuoles is required for autophagic degradation in the yeast,Saccharomyces cerevisiae." J.Biochem.193(in press). (1997)
• [Publications] Funakoshi,T.et al,: "Analyses of APG13 gene involved in the autophagy in yeast,Saccharomyces cerevisiae." Gene. 179(in press). (1997)
• [Publications] Matsuura,A.et al,: "Apglp,a novel protein kinase required for the autophagic process in Saccharomyces cerevisiae." Gene. 179(in press). (1997)
• [Publications] 大隅良典: "生化学" 日本生化学会, 6 (1996)