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Function of actin filaments in organization of cortical microtubules of plant ce

Research Project

Project/Area Number 07454221
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionHimeji Institute of Technology

Principal Investigator

SONOBE Seiji  Himeji Institute of Technology, Faculty of Science, associate professor, 理学部, 助教授 (30197024)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥7,800,000 (Direct Cost: ¥7,800,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1995: ¥6,300,000 (Direct Cost: ¥6,300,000)
Keywordsactin filament / microtubules / Tobacco BY-2 Cells / microtubule-associated protein / actin-bindring protein / Hydrocharis / root hair / Cytoplasmic streaming / 植物細胞 / アクチン結合タンパク質 / タバコ培養細胞BY-2 / 微小管 / タンパク質精製 / 架橋タンパク質
Research Abstract

In order to clarify the function of actin filaments in the organization of cortical microtubules, we tried to identify a protein (s) which could bind to both microtubules and actin filaments. Crude cytoplasmic extract was prepared from evacuolated protoplasts of tobaco BY-2 cells and tubulin and actin were polymerized upon addition of taxol and phalloidin and the polymers were collected. After depolymerization of microtubules followed by repolymerization, microtubule-associated protein fraction were obtained by salt extraction and the fraction was further fractionated by MonoQ column. On the other hand, we also tried to purify tubulin and atin and succeeded in their purification. An monoclonal antibody specific to plant actin could be prepared. Cosedimentation experiments using purified microtubules and actin filaments demonstrated that 120 and 90 kDa polypeptides could bind to both microtubules and actin filaments. These polypeptides induced bundle formation of actin filaments. Now, we are trying to prepare antibodies against these polypeptides to investigate colocalization of them with cortical microtubules. On the other hand, organization of actin filaments in Hydorcharis root hair cells was studied. In the cortical region, actin filaments colocalized with microtubules and reconstruction of actin filaments in ordered array was inhibited in the absence of microtubules, suggesting that arrangement of actin filaments was regulated by microtubules in Hydrocharis root hair.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Yasuhara,H.: "Effects of brefeldin A on the formation of the cell plate in tobacco BY-2 cells." Eun.J.Cell Biol.66. 274-281 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yokota,E.: "Plant microtubules can be translocated by a dynein ATPase from sea urchin in vitro." Plant Cell Physiol.36. 1563-1569 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shibaoka,H.: "The use of model systems prepared from tobacco BY-2 cells for studies of the plant cytoskeleton." J.Microscopy. 181. 145-152 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Higashiyama,T.: "Identification of a novel 70kDa protein in cultures tobacco cells that is immunologically related to MAP4." Cytologia. 61. 229-233 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sonobe,S.: "Studies on the plant cytoskeleton using miniprotoplasts of tobacco BY-2 cells." J.Plant Res.109. 437-448 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasuhara, H., Sonobe, S.and Shibaoka, H.: "Effects of brefeldin A on the formation of the cell plate in tobacco BY-2 cells." Eur. J.Cell Biol. 66. 274-281 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yokota, E., Sonobe, S., Igarashi, H.and Shimmen, T.: "Plant microtubules can be translocated by a dynein ATPase from sea urchin in vitro." Plant Cell Physiol. 36. 1563-1569 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shibaoka, H., Asada, T., Yamamoto, S.and Sonobe, S.: "The use of model systems prepared from tobacco BY-2 cells for studies of the plant cytoskeleton." J.Microscopy. 181. 145-152 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Higashiyama, T., Sonobe, S., Murofushi, H., and Hasezawa, S.: "Identification of a novel 70 kDa protein in cultures tobacco cells that is immunologically related to MAP 4." Cytologia. 61. 229-233 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sonobe, S.: "Studies on the plant cytoskeleton using miniprotoplasts of tobacco BY-2 cells." J.Plant Res. 109. 437-448 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shibaoka, H.: "The use of model systems prepared from tobacco BY-2 cells for studies of the plant cytoskeleton." J. Microscopy. 181. 145-152 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Higashiyama, T.: "Identification of a novel 70 kDa protein in cultures tobacco cells that is immunologically related to MAP 4." Cytologia. 61. 229-233 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sonobe, S.: "Studies on the plant cytoskeleton using miniprotoplasts of tobacco BY-2 cells." J. Plant Res.109. 437-448 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yasuhava H.: "Effects of brefeldin A on the formation of the cell plate in tobacco BY-2 cells" Eur.J.Cell Biol.66. 274-281 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yokota E.: "Plant microtubules can be translocated by a dynein ATPase from sea urchin in vitro" Plant Cell Physiol.36. 1563-1569 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shibaoka H.: "The use of model systems prepared from tobacco BY-2 cells for studies of the plant cytoskeleton" J.Microscopy. (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] 園部誠司: "植物の分子細胞生物学・(細胞分裂におけるフラグモプラストの機能)" (株)秀潤社, 228(12) (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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