| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Research Abstract |
In the last year, we coult successfully develop a new methodology for chiral recognition in the highly structured host-guest complexation systems, which we called as "FABMS/EL (Enantiomer-Labeled) guest method", and clarify the fundamental aspects of this newly developed method. Chiral recognition ability was evaluated by the IRIS values : I [(H+G_R) ^+] /I [(H+G_<S-dn>) ^+].
In this year, (i) we applied this method to various host molecules, and (ii) we extended this method to ESI (Electrospray Ionization) mass spectrometry, that is, "ESIMS/EL guest method".
(1) As the results of "FABMS/EL guest method", it was concluded that various chiral crown ethers, carbohydrates, and antibiotics exhibit various degree of chiral recognition toward various amino acids their esters within the range of IRIS=5.0-0.2.
(2) As the results of "ESIMS/EL guest method", it was conculuded that ESIMS provides certain depressed IRIS values compared with FABMS so that cautions are required for evaluating chiral recognition ability in a quantitative fashion.
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