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Fundamental study on gene-hyperthermia for cancer using heat sensitive gene

Research Project

Project/Area Number 07455326
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物・生体工学
Research InstitutionNagoya University

Principal Investigator

HONDA Hiroyuki  School of Engineering, Nagoya University Associate Professor, 工学部, 助教授 (70209328)

Co-Investigator(Kenkyū-buntansha) WAKABAYASHI Toshihiko  School of Medicine, Nagoya University Assistant Professor, 医学部, 助手 (50220835)
YOSHIDA Jun  School of Medicine, Nagoya University Professor, 医学部, 教授 (40158449)
KOBAYASHI Takeshi  School of Engineering, Nagoya University Professor, 工学部, 教授 (10043324)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1996: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1995: ¥4,100,000 (Direct Cost: ¥4,100,000)
Keywordshyperthermia / magnetite particle / liposome / tumor / interferon / gene
Research Abstract

Hyperthermia for cancer using magnetite particles as heating material has been studied. In the present study, hyperthermia was not performed only by heating alone but also by combination with gene expression and drug incorporation.
In order to increase the amount of magnetite adsorbed to a cancer cell, TMAG which is a lipid with positive charge was incorporated to a magnetoliposome. Polyethylene glycol coated magnetite particles were prepared and those amount it tumor tissue was observed by means of Magnetic Resonance Imaging after administrated through a tail vein. It was found that those particles can be used as a magnetic resonance contrast agent. Interferon beta gene was arranged downstream heat inducible promoter and the liposome with the gene was prepared. When the gene-encapsulated liposome was incorporated to glioma cells, the transient expression of interferon-beta was obtained. In order to enhance the killing effect by hyperthermia, the magnetoliposome prepared by us was directly administrated to the tumor tissue, which formed under the skin of a rat. Glioma tissue was observed histologically after 40 min treatment by high efficiency magnetic field with 386 Oe. As a result, it was found that almost all rat glioma cells were dead after 3 times treatment.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 鈴木基司ら: "Development of a target-directed magnetic resonance contrast agent using monoclonal antibody-conjugated magnetic particles" Brain Tumor Pathology. 13. 127-132 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 新海政重ら: "Intracellular hyperthermia for cancer using magnetite cationic liposome-in vivo study-" Japanese Journal of Cancer Research. 87. 1179-1183 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Isabelle Anne Bouhonら: "Enhancement of cytokine expression in transiently transfected cells by magnetoliposome mediated hyperthermia" Cytotechnology. 22. (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 新海政重ら: "Thermochemotherapy for canser using antitumor drugs-encapsulated magnetoliposome" Japanese Journal of Cancer Research. 88. (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Motoshi Suzuki, Hiroyuki Honda, Takeshi Kobayashi: "Development of a target-directed magnetic resonance contrast agent using monoclonal antibody-conjugated magnetic particles" Brain Tumor Pathology. 13(4). 127-132 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Masashige Shinkai, Mitsugu Yanase, Hiroyuki Honda, Toshihiko Wakabayashi, Jun Yoshida, Takeshi Kobayashi: "Intracellular hyperthermia for cancer using magnetite cationic liposome-in vivo study-" Japanese Journal of Cancer Research. 87(11). 1179-1183 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Isabelle Anne Bouhon, Masashige Shinkai, Hiroyuki Honda, Takeshi Kobayashi: "Enhancement of cytokine expression in transiently transfected cells by magnetoliposome mediated hyperthermia" Cytotechnology. 22. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Masashige Shinkai, Nobukazu Yokoi, Wataru Shimizu, Hiroyuki Honda, Takeshi Kobayashi: "Thermochemotherapy for canser using antitumor drugs-encapsulated magnetoliposome" Japanese Journal of Cancer Research. 88. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 鈴木基司ら: "Development of a target-directed magnetic resonance contrast agent using monoclonal antibody-conjugated magnetic particles" Brain Tumor Pathology. 13・4. 127-132 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 新海政重ら: "Intracellular hyperthermia for cancer using magnetite cationic liposome -in vivo study-" Japanese Journal of Cancer Research. 87・11. 1179-1183 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Isabelle Anne Bouhonら: "Enhancement of cytokine expression in transiently transfected cells by magnetoliposome mediated hyperthemia" Cytotechnology. 22. (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 新海政重ら: "Thermochemotherapy for canser using antitumor drugs-encapsulated magnetoliposome" Japanese Journal of Cancer Research. 88. (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 新海 政重ら: "Antibody-conjugated magnetolipsomes for targeting cancer cells and their application in hyperthermia" Biotechnology and Applied Biochemistry. 21. 125-137 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 鈴木 基司ら: "Preparation and characteristics of magnetite-labelled antibody with the use of poly (enthylene glycol) derivatives" Biotechnology and Applied Biochemistry. 21. 335-346 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 鈴木 基司ら: "Development of a contrast agentfor magnetic resonance imaging of tumor xenogratts using target-directed magnetic particles" Brain Tumor Pathology. (in press). (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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