| Project/Area Number |
07456003
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Institute of Molecular and Cellular Biosciences, University of Tokyo |
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Principal Investigator |
UCHIMIYA Hirofumi University of Tokyo, Institute of Molecular and Cellular Biosciences, professor, 分子細胞生物学研究所, 教授 (50142229)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 1996: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1995: ¥5,200,000 (Direct Cost: ¥5,200,000)
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| Keywords | rice / stress / salt / gene expression / mRMA / nucleotide / homeostasis / DNA / 塩ストレス / ストレス耐性 / エネルギー代謝 / 通気組織 / プロテアソーム / タンパク質分解 |
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| Research Abstract |
Developmental and some biochemical responses of rice (Oryza sativa L.) seedlings to salt stress were studied. Shoot and more critically root growth were limited when the seedlings were exposed to moderate levels of salinity (NaCl). Anatomical observations revealed that under normal growth conditions, aerenchyma formation in rice roots occurred 3 days after seed germination. Development of aerenchyma was also accompanied by well developed sclerenchyma and vascular tissues in roots. In contrast, under salt stress aerenchyma formation was not observed in roots of comparable growth stages. Detailed histological observations showed the developmental retardation of sclerenchyma and vascular elements by salt stress. Salinity stress also caused several other anatomical changes of root tissues with regard to secondary wall formation in vascular tissues and some UV-fluorescent substances observed exclusively associated with sclerenchyma and vascular tissues.
Comparison of protein profiles from salt-treated and untreated root tissues indicated that the synthesis of several polypeptides was significantly suppressed by salt stress. Appearance of these in normal tissues could be correlated with aerenchyma development. Data on further analysis using antibodies of several proteins and electron microscopy are presented.
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