| Project/Area Number |
07456020
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
園芸・造園学
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
OKUBO Hiroshi KYUSHU UNIVERSITY,FACULTY OF AGRICULTURE,ASSOCIATE PROFESSOR, 農学部, 助教授 (80150506)
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| Co-Investigator(Kenkyū-buntansha) |
OZAKI Yukio KYUSHU UNIVERSITY,FACULTY OF AGRICULTURE,ASSISTANT PROFESSOR, 農学部, 助手 (60253514)
MIYAJIMA Ikuo KYUSHU UNIVERSITY,FACULTY OF AGRICULTURE,ASSISTANT PROFESSOR, 農学部, 助手 (20182024)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥5,300,000 (Direct Cost: ¥5,300,000)
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| Keywords | Wakegi / Shallot / Intraspecific differentiation / Isozymes / DNA / フラボノイド色素 |
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| Research Abstract |
Grouping of the 228 accessions of shallot or wakegi collected from Asian countries based on morphological and flowering traits was conducted. Almost of all the collected accessions were classified into shallot or wakegi.
Discrimination of shallot from wakegi by isozyme analysis clarified that all the accessions from Japan, Korea and Taiwan were wakegi, those from Thailand, Malaysia and Bangladesh were shallot, and those from Indonesia were mixture of shallot and wakegi. Particularly, the genetic diversity in shallot and wakegi was distinct in West Java and South Sumatra, Indonesia. It is suggested that one of the places of origin of wakegi may be thought to be this area. RAPD analysis also clarified the genetic diversity of the crops in this area. Chloroplast DNA analysis clarified that most of wakegi accessions were the hybrid of Welsh onion * shallot, but that there are a few accessions derived from shallot as maternal and Welsh onion as paternal parents.
Major anthocyanins in the accessions were cyanidin and only quercetin was found as flavonol. The ratio of the flavonol to anthocyanins was different in shallot and wakegi, and the stableness of color appearance under UV lights was different by accessions. There were also diversity in response to daylength and temperature for bulb formation and in depth of bulb domancy. These physiological traits were highly related to the classification by isozyme and DNA analyzes.
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