Which is the origin of the Phytoplasma, Plants or Insects?
| Project/Area Number |
07456026
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物保護
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NUMBA Shigetou The University of Tokyo, Graduate School of Agriculture and Agricultural Life Sciences, Professor, 大学院・農学生命科学研究科, 教授 (50189221)
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| Co-Investigator(Kenkyū-buntansha) |
魯 暁云 日本学術振興会, 特別研究員
ISHIKAWA Yukio The University of Tokyo, Graduate School of Agriculture and Agricultural Life Sc, 大学院・農学生命科学研究科, 助教授 (60125987)
LU Xiaoyun Japan Society of the Promotion of Science, Research fellow
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥7,900,000 (Direct Cost: ¥7,900,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1995: ¥6,500,000 (Direct Cost: ¥6,500,000)
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| Keywords | Phytoplasma / 16rRNA gene / CTAB / Bootstrap / Macrosteles striifrons / Scleroracus flavopictus |
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| Research Abstract |
We have analyzed phytplasma in terms of molecular evolutionism, and studied in order to establish systems of genome analysis and molecular diagnosis. First, we gathered various separated roots of phytoplasma and refined the genome by the CTAB method. Then we amplified 16SrRNA genes by PCR and determined the whole base arrangemant by direct sequences. Further, we refered the newest base data of 16SrRNA genes in microbes including phytoplasma in other countries, using data base of the large computer, and compared the results to the base arrangement data we had before. Therefore, We have constructed the molecular genealogical family tree by large analysis program "ODEN" and proceeded "Bootstrap" to analyze reliablility. Under the analyzed data, we have established the classifying system of phytoplasma. Further, we have applyed the classification of "species" to phytoplasma by the above data and system, designed and composed the unique primer. Also we have tried to classify the unknown separated roots of phytoplasma in the various area of Asia.
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Report
(3 results)
Research Products
(12 results)
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[Publications] Sato,M.,Namba,S.,Katsuhara,M,Kawakita,H.,Mitsuhashi,W & Kawabe,S:"PCR(Polymerase Chain Reaction)Detection of Phytoplasema in Phloem Sap Collected by Laser Stylectomy" 日本植物病理学会報. 62・2. 177-180 (1996)
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