| Project/Area Number |
07456030
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物保護
|
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| Research Institution | Kobe University |
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Principal Investigator |
OHKAWA Hideo Kobe University, Faculty of Agriculture, Professor, 農学部, 教授 (90233048)
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| Co-Investigator(Kenkyū-buntansha) |
IMAISHI Hiromasa Kobe University, Faculty of Agriculture, assistant, 農学部, 助手 (50223318)
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| Project Period (FY) |
1995 – 1997
|
| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥4,600,000 (Direct Cost: ¥4,600,000)
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| Keywords | Herbicide Resistance / Chloroplast / Transgenic tobacco / P450 Monooxygenase / チトクロームP450 / 光合成電子伝達系 / ラットP4501A1 / クロロプラスト輸送 / トランスジェニック・タバコ / 薬物代謝 / RuBisCO スモ-ルサブユニット・プロモーター / 7-エトキシクマリンO-脱エチル化 |
|---|
| Research Abstract |
The transgenic tobacco plants expressing rat P4501A1 and its fused enzyme with yeast P450 reductase in chloroplants were generated by genetic engineering. P4501A1 and its fused enzyme were coupled with photosynthetic electron transport chains in the chloroplants to exhibite an enhanced monooxygenase activity under light irradiation. The transgenic tobacco plants metabolized the herbicide chlorotoluron through N-demethylation and m-methyl hydroxylation to exhibite herbicide resistance. Particularly, m-methyl hydroxylation seemed to be important for herbicide resistance.
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