| Project/Area Number |
07456033
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
蚕糸・昆虫利用学
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
NAGATA Masao The University of Tokyo, Graduate School of Agriculture and Agricultural Life Sciences, Associate Professor, 大学院・農学生命科学研究科, 助教授 (70107407)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OZAKI Masataka The University of Tokyo, Graduate School of Agriculture and Agricultural Life Sc, 大学院・農学生命科学研究科, 助手 (00270893)
SHIMADA Toru The University of Tokyo, Graduate School of Agriculture and Aagricultural Life S, 大学院・農学生命科学研究科, 助教授 (20202111)
KOBAYASHI Masahiko The University of Tokyo, Graduate School of Agriculture and Agricultural Life Sc, 大学院・農学生命科学研究科, 教授 (60162020)
|
|---|
| Project Period (FY) |
1995 – 1996
|
| Project Status |
Completed (Fiscal Year 1996)
|
| Budget Amount *help |
¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 1996: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥5,700,000 (Direct Cost: ¥5,700,000)
|
|---|
| Keywords | Silkworm / Virus / Infection / Nuclear Polyhedrosis Virus / NPV |
|---|
| Research Abstract |
1.We detected differences in cytopathological changes with infection of BmNPV between two cultivated cell lines, BmN and l30 from the silkworm. The analyzes showed that differences existed in infectivity, multiplication rate and activity the virus and cell lethality between two cell lines. Apoptosis, which did not occur in BmN,was observed in l30 cells.
2.A few cells survived in l30 cells after infection of high concentration of BmNPV.The cell lines cloned from them had various cytological and pathological characters, but their resistance to the virus was not high.
3.Polyhedral protein synthesis did not correlate to particle replication of NPV,when amounts of plyhedral and virus particle proteins were measured under various conditions of infection with cell density and multiplicity of infection.
4.Injury effects of the larval hemolymph on cultivated cells was observed and lipophorin was identified to be one of the injury factors.
5.Ecdysteroid feeding to larvae at low concentrations had no effect on multiplication of NPV and cytoplasmic polyhedrosis virus.
|
