Establishment of the methodology to determine nutritional status in higher plants using molecular biological approaches

• Project/Area Number: 07456037
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: THE UNIVERSITY OF TOKYO
• Principal Investigator: CHINO Mitsuo The University of Tokyo, Graduate School of Agriculrural and Life Sciences, Professor, 大学院・農学生命科学研究科, 教授 (10007677)
• Co-Investigator(Kenkyū-buntansha): FUJIWARA Toru The University of Tokyo, Graduate School of Agriculrural and Life Sciences, Assi, 大学院・農学生命科学研究科, 助手 (80242163) ; HAYASHI Hiroaki The University of Tokyo, Graduate School of Agriculrural and Life Sciences, Asso, 大学院・農学生命科学研究科, 助教授 (60180973)
• Project Period (FY): 1995 – 1996
• Project Status: Completed (Fiscal Year 1996)
• Budget Amount: ¥6,900,000 (Direct Cost: ¥6,900,000); Fiscal Year 1996: ¥2,100,000 (Direct Cost: ¥2,100,000); Fiscal Year 1995: ¥4,800,000 (Direct Cost: ¥4,800,000)
• Keywords: Molecular Biology / Plant Nutrition / Nutritional Status / Regulation of Gene Expression / Sulfur Nutrition / Metallothionein / Boron Nutrition / Transgenic Plants / 遺伝子発現 / 栄養診断 / ディフレンシャルディスプレイ / 植物栄養 / 微量要素

Research Abstract

Three major experiments were conducted under this projects.
1) A fragment cDNA corresponding to a gene of Arabidopsis regulated by boron nutrition was isolated by the method called differential display. The methods utilizes the PCR procedure to compare population of two or more mRNA populations.
2) Arabidopsis metallothionein gene, which we previously isolated in the lab, was found to respond to the elevated levels of Cu in roots but not in leaf tissues. Anaerobic stresses were also found to be involved in the regulation processes.
3) The gene encoding the beta subunit of beta conglycinin, a major seed storage protein of soybean, was found to respond to sulfur and nitrogen nutrition. A DNA fragment in the promoter responsible for the regulation was identified.

Research Products

• [Publications] T.Fujiwara et al.: "Ragulation of Soybean Seed Strage Protein Gene-A Model for Sulfur Nutrition-Regulated Gene Expression" Sulfur Nutr.and Assimilation,ed Gram J.,SPB Academic Pross.(in press). (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] M.Awazuhara et al.: "Changes in Metallothion air.gene expression in Response to Sulfur Nutrition in C&d Mutant." Sulfur Nutr.and Assimilation,ed Gram J.,SPB Academic Press.(in press). (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] M.YOKOTA-Hrai.et al.: "Effect of Nitrogen Nutrition on the Exprossion of a Soybean Seed Storage Protein Gene in Trausgeric Arobidpodsis thaliana" Sulfur Nutr.and Assimilation,ed Cram J.,SPB Academic Pross.(in press). (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Toru Fujiwara, Hirai Yokota Masami, Hoyeun Kim, Motoko Awazuhara, Chikako Honda, Akifusa Matsui, Akio Furuhashi, Nakako Shibagaki, Miho Yasumori, Hiroaki Hayashi, Mitsuo Chino, and Satoshi Naito: "REGULATION OF SOYBEAN SEED STORAGE PROTEIN GENES-A MODEL FOR SUFUR NUTRITION-REGULATED GENE EXPRESSION" Sulfur nutrition and assimilation in higher plants, Cram J ed., SPB academic Publishing, Hage. (in press). (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Masami Yokota Hirai, Toru Fujiwara, Mitsuo Chino and Satoshi Naito: "EFFCTS OF NITOGEN NUTRITION ON THE EXPRESSION OF A SOYBEAN SEED STORAGE PROTEIN GENE IN TRANSGENIC ARABIDOPSIS THALIANA" Sulfur nutrition and assimilation in higher plants, Cram J ed., SPB academic Publishing, Hague. (in press). (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Motoko Awazuhara, Eiichi Akahoshi, Toru Fujiwara, Mitsuo Chino: "CHANGES IN METALLOTHINEIN GENE EXPRESSION IN RESPONSE TO SULFUR NUTRITION IN cad MUTANTS" Sulfur nutrition and assimilation in higher plants, Cram J ed., SPB academic Publishing, Hague. (in press). (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Wei-Ming Shi et al.: "Effect of CaCN_2 treatment of Seed of Inubie on Synthesis of Protein and RNA during germination" Soil Science Plant Nutrition. 42・3. 443-449 (1996)
• [Publications] 藤原徹: "イオウ栄養に応じた遺伝子発現制御" RADIOISOTOPES. 46. 59-60 (1997)
• [Publications] M.Yokota-Hirai et al.: "Effects of nitrogen nutrition on the expression of a soybean seed storage proteuns gene in trangenic Anabidopsis" SPB Academio Publishing,Hague. (in press).