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Analysis of early response and tolerance mechanism to Al stress in plant.

Research Project

Project/Area Number 07456040
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionOkayama University

Principal Investigator

MATSUMOTO Hideaki  Res.Inst.for Bioresources, Okayama Univ., Professor, 資源生物科学研究所, 教授 (80026418)

Co-Investigator(Kenkyū-buntansha) MA Jian Feng  Res.Inst.for Bioresources, Okayama Univ., Research Associate, 資源生物科学研究所, 助手 (80260389)
YAMAMOTO Yoko  Res.Inst.for Bioresources, Okayama Univ., Associate Professor, 資源生物科学研究所, 助教授 (50166831)
江崎 文一  岡山大学, 資源生物科学研究所, 助手 (90243500)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥6,100,000 (Direct Cost: ¥6,100,000)
KeywordsAcid soil / Aluminum stress / Injury / Tolerant mechanism / Organic acid / Exclusion / Tolerant plant / 有機酸分泌 / 耐性遺伝子 / 酵母 / 根伸長阻害 / アルミニウム毒性 / アルミニウム耐性 / リグニン / 細胞死 / 脂質過酸化 / 鉄イオン
Research Abstract

The internal detoxification mechanism of aluminum was investigated in an Al-accumulating plant, Hydrangea macrophylla, focusing on Al forms present in the cells. The leaves of Hydrangea macrophylla contained as high as 15.7 mmol Al kg ^<-1> in fresh weight, and more than two thirds of the Al was found in the cell sap. Using ^<27> Al-nuclear magnetic resonance (NMR), the dominant peak of Al was observed at a chemical shift of 11 to 12 ppm in both the intact leaves and extracted cell sap, which is in good accordance with the chemical shift for the 1 : 1 Al-citrate complex. Purification of cell sap by molecular sieve chromatography (Sephadex G-10), combined with ion-exclusion chromatography indicated that Al in fractions with the same retention time as citric acid contributed to the observed ^<27>Al peak in the intact leaves. The molar ratio of Al to citric acid in the crude and purified cell sap approximated 1. The structure of the ligand chelated with Al was further examined by mass spectrometry and ^1 H-nuclear magnetic resonance after purification, and the molecular weight, chemical shifts and coupling constants were the same as those of citric acid. Bioassay experiments showed that the purified Al complex from the cell sap did not inhibit root elongation of corn (Zea mays L.) and the viability of cells on the root tip surface was also not affected. These observations indicated that Al is bound to citric acid in the cell of hydrangea leaves. Therefore, formatin of strong Al-citrate complex, a non-toxic species of Al, is an internal detoxification mechanism of Al in hydrangea leaves.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Ma,J.F.: "Al-induced inhibition of root elongation in corn,Zea mays L.is overcome by Si addition." Plant Soil. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ma,J.F.: "Internal detoxification mecahnism of aluminum in Hydrangea macrophylla. Identification of Al form in the leaves." Plant Physiology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ezaki,B.: "Expression of a moderately anionic peroxidase is induced by aluminum treatment in tobacco cells ; Possible involvement of peroxidase isozymes in aluminum ion stress." Physiologia Plantarum. 96. 21-28 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsumoto,H.: "Response of the plant root to aluminum stress : analysis of the inhibition of the root elongation and changes in membrane function." J.Plant Research. 109. 99-105 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sasaki,M.: "Lignin deposition induced by aluminum in wheat (Triticum aestivum) roots." Physiologia Plantarum. 96. 193-198 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamamoto,Y.: "Tolerance to aluminum acquired during phosphate starvation of cultured tobacco cells." Plant Physiology. 112. 217-227 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsumoto, H., et al.: "The mechanisms of Al toxicity and tolerance in higher plants." Proc.International Sympolsium of Biotechnology and Biodiversity.Oxford and IBH Publishing Co.New Delhi.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ma, J.F., et al.: "Al-induced inhibition of root elongation in corn, Zea mays L.is overcome by Si addition." Plant Physiol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sasaki, M., et al.: "Aluminum inhibits growth and stability of cortical microtubules in wheat (Triticum aestivum) roots." Soil Sci.Plant Nutr.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sasaki, M., et al.: "Lignin deposition induced by aluminum in wheat (Triticum aestivum) roots." Physiol.Plant.96. 193-198 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ezaki, B., et al.: "Expression of a moderately anionic peroxidase is induced by aluminum treatment in tobacco cells ; Possible involvement of peroxidase isozymes in aluminum ion stress." Physiol.Plant.96. 21-28 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsumoto, H., et al.: "Response of the plant root to aluminum stress : analysis of the inhibition of the root elongation and changes in membrane function." J.Plant Res.109. 99-105 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamamoto, Y., et al.: "Tolerance to aluminum acquired during phosphate starvation of cultured tobacco cells." Plant Physiol.112. 217-227 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ma,J.F.: "Al-induced inhibition of root elongation in corn,Zea mays L.is overcome by Si addition." Plant Soil. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Ma,J.F.: "Internal detoxification mecahnism of aluminum in Hydrangea macrophylla. Identification of Al form in the leaves." Plant Physiology. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Ezaki,B.: "Exoression of a moderately anionic peroxidase is induced by aluminum treatment in tobacco cells ; Possible involvement of peroxidase isozymes in aluminum ion stress." Physiologia Plantarum. 96. 21-28 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mastumoto,H.: "Response of the plant root to aluminum stress : analysis of the inhibition of the root elongation and changes in membrane function." J.Plant Research. 109. 99-105 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sasaki,M.: "Lignin deposition induced by aluminu in wheat (Triticum aestivum) roots." Physiologia Plantarum. 96. 193-198 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamamoto,Y.: "Tolerance to aluminum acquired during phosphate starvation of cultured tobacco cells." Plant Physiology. 112. 217-227 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ezaki,B: "Cloning and sequencing of the cDNAs induced by aluminium treatment and Pi starvation in cultured tobacco cells." Physiol.Plant. 93. 11-18 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ono,K.: "Synergistic inhibiton of growth by aluminum and iron of tobacco (Nicotiana tabacum L.) cells in suspension culture." Plant Cell Physiol.36. 115-125 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sasaki,M: "Involvement of plasma membrane potential in the tolerance mechanism of plant roots to aluminium toxicity." Plant and Soil. 171. 119-124 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ezaki,B: "Expression of a moderately anionic peroxidase is induced by aluminum treatment in tobacco cells ; Possible involvement of peroxidase isozymes in aluminum ion stress." Physiol.Plant (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Matsumoto,H.: "Response of the plant root to aluminum stress : analysis of the inhibition of the root elongation and changes in membrane function." J.Plant Res.(in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Sasaki,M.: "Lignin deposition induced by aluminum in wheat (Triticum aestivum) roots." Physiol.Plant (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Kasai,M.: "Plant-Soil Interactions at Low pH : Principles and Management (Date R.A.et al.eds)" Kluwer Academic Publishers, 4(341-344) (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yamamoto,Y.: "Plant-Soil Interactions at Low pH : Principles and Management (Date R.A.et al.eds)" Kluwer Academic Publishers, 3(359-361) (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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