TOKUYAMA Tastuaki Nihon University, department of Agricultural andBiological Chemistry, 生物資源科学部, 教授 (90059684)
OGAWA Hitoshi Tamagawa University, Faculty of Agriculture, Lecturer, 農学部, 講師 (50241047)
WAKABAYASHI Ko Tamagawa University, Faculty of Agriculture, Professor, 農学部, 教授 (00220830)
|Budget Amount *help
¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1996: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥3,000,000 (Direct Cost: ¥3,000,000)
(1)In spite that it is difficult to prepare 1,3,5-triazines having different substituents, 2,4-bis(trichloromethy1)-6-trifluoromethy 1-1,3,5-triazine and 2-methy 1-4-trichloromethy 1-6-trifluoromethy 1-1,3,5-triazine were synthesized by the condensation reaction between corresponding imidoy 1-amidines and acid anhydrides. Other many of 1,3,5-triazines were synthesized using trimerization reaction and co-trimerization reactions.
(2)1,3,5-Triazines having fluorine substituents showed weak nitrification inhibitory activity but 2-amino-4-tribromomethy 1-6-trichloromethy 1-1,3,5-triazine very strongly suppressed the growth of Nitrosomonas europaea ATCC25978 (pI_<50>=7.12). It was 65 times stronger than that of authentic nitrification inhibitor, MAST (pI_<50>=5.31).
(3)We made a new ammonia bicarbonate (ABC) medium for the culture of N.europaea ATCC25978. In this medium N.europaea grew 3 times faster than in Lewis & Pramer medium.
(4)Attempt to isolate new nitrifying bacteria using ABC medium from soil is not sucessful yet.
(5)In the bioassay using N.europaea ATCC25978, N.sp.TK794, Nitorobacter agilis ATCC14123, and N.sp TH21., synthesized 1,3,5-triazines inhibited the growth of genus Nitorosomonas.
(6)In the cell free level assay of O_2-consumption by N.europaea using ammonium sulfate as substrates, the target enzyme of synthesized 1,3,5-triazines is found to be an ammonia-monooxygenase.