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Chemical Structures and Functions of Neurpeptides in crustaceans

Research Project

Project/Area Number 07456054
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionOcean Research Institute, The University of Tokyo

Principal Investigator

NAGASAWA Hiromichi  The University of Tokyo, Ocean Research Institute, Professor, 海洋研究所, 教授 (60134508)

Co-Investigator(Kenkyū-buntansha) WATANABE Toshiki  The University of Tokyo, Ocean Research Institute, Associate Professor, 海洋研究所, 助教授 (00272526)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1996: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1995: ¥4,800,000 (Direct Cost: ¥4,800,000)
KeywordsCrustacea / Penaeus japonicus / peptide hormone / hyperglycenic hormone / molt-inhibiting hormone / vitellogenesis-inhibiting hormone / red pigment concentrating hormone / pigment disparsing hormone / 甲殼類 / アメリカザリガニ / 神経ペプチド
Research Abstract

Seven molecular species of crustacean hyperglycemic hormone (CHH)-family peptides (Pej-SGP-I-VII), a red pigment concentrating hormone (RPCH), and two pigment dispersing hormones (PDHs) were isolated and their complete amino acid sequences determined. Like CHH-family peptides from other crustacean species, the seven CHH-family peptides consisted of 72-77 amino acid residues with six conserved Cys residues.
Six peptides (Pej-SGP-I,II,III,V,VI and VII) showed hyperglycemic activity, when injected into eyestalk-ablated prawns, while Pej-SGP-IV exhibited inhibitory activity in the release of molting hormone (ecdysteroids) by the Y organ cultured in vitro. V and VI also showed a weak molt-inhibiting activity. These peptides were subjected to another assay to assess vitellogenesis-inhibiting activity, in which the effects of these peptides on the protein synthesis were examined by using pieces of developing ovary cultured in vitro. Six peptides with hyperglycemic activity showed inhibitory activity in the protein synthesis. The inhibition was not specific to only synthesis of vitellogenin, but that of all the proteins was inhibited.
All the six peptides with hyperglycemic activity shared carboxyl-terminal amide structure. In order to clarify the impotance of this structure, we tried to synthesize a carboxyl-terminal free peptide by silkworm baculovirus expression system. Double-stranded DNAs encoding Pej-SGP-III and its analog with free carboxyl terminaus were chemically synthesized and inserted into virus DNA,which were injected into 5th instar larvae of the silkworm. But we could not detect any III or its analog in the hemolymph.
The amino acid sequence of RPCH of this species was identical to that of other species thus far obtained. Two PDHs, with three different residues to each other, were equally potent in pigment dispersing activity. Erythrophore was most sensitive to these hormones, xanthophore and melanophore were less sensitive, and leucophore was least sensitive.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Wei-Jun Yang: "Amino acid sequence of a peptide with molt-inhibiting activity from the kuruma prawn penaeus japonicus" Peptides. 17. 197-202 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiromichi Nagasawa: "Isolation and amino acid sequence of a molt-inhibiting hormone from the American crayfish,Procambarus clarkii" Biosci.Biotech.Biochem.60. 554-556 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akiko Terauchi: "Identification of a molt-inhibiting hormone from the sinus gland of the crayfish,Procambarus clarkii" Zool.Sci.13. 295-298 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsuyoshi Ohira: "Cloning and sequence analysis of a cDNA encoding a crustacean hyperglycemic hormone from the kurumaprawn penaeus japonicus" Mol.Mar.Biol.Biotech.(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wei-Jun Yang: "Amino acid sequences and activities of multiple hyperglycemic hormones from the kuruma parawn,Penaeus japonicus" Peptides. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wie-Jun Yang et al.: "Amino acid sequence of a peptide with molt-inhibiting activity from the kuruma prawn Penaeus japonicus" Peptides. 17. 197-202 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiromichi Nagasawa et al.: "Isolation and amino acid sequence of a molt-inhibiting hormone form the American crayfich, Procambarus clarkii" Biosci.Biotech.Biochem.60. 554-556 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akiko Terauchi et al.: "Identification of a molt-inhibiting hormone from the sinus gland of the crayfish, Procambarus clarkii" Zool.Sci.13. 295-298 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsuyoshi Ohira et al.: "Cloning and sequence analysis of a cDNA encoding a crustacean hyper-glycemic hormone from the kuruma prawn, Penaeus japonicus" Mol.Mar.Biol.Biotech.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wei-Jun Yang et al.: "Amino acid sequences and activities of multiple hyperglycemic hormones form the kuruma prawn, Penaeus japonicus" Peptides. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wei-Jun Yang: "Amino acid sequence of a peptide with molt-inhibiting activity from the kuruma prawn Penaeus japonicus" Peptides. 17. 197-202 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hiromichi Nagasawa: "Isolation and amino acid sequence of a molt-inhibiting hormone from the American crayfish,Procambarus clarkii" Biosci.Biotech.Biochem.60. 554-556 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Akiko Terauchi: "Identification of a molt-inhibiting hormone from the sinus gland of the crayfish,Procambarus clarkii" Zool.Sci.13. 295-298 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tsuyoshi Ohira: "Cloning and sequence analysis of a cDNA encoding a crustacean hyperglycemic hormone from the kuruma prawn Penaeus japonicus" Mol.Mar.Biol.Biotech.(in press). (1977)

    • Related Report
      1996 Annual Research Report
  • [Publications] Wei-Jun Yang: "Amino acid sequences and activities of multiple hyperglycemic hormones from the kuruma prown,Penaeus japonicus" Peptides. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Wei-jun Yang et al.: "Amino acid sequences of a hyperglycaemic hormone and its related peptides from the Kuruma prawn" Aquaculture. 135. 205-212 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Wei-jun Yang et al.: "Amino acid sequences of a peptide with molt-inhibiting activity from the Kuruma prawn Penaeus japonicus" Peptides. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hiromichi Nagasawa et al.: "Isolation and amino acid sequence of a molt-inhibiting hormone from the American crayfish, Procambarus clarkii" Biosci. Biotech. Biochem.(印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Akiko Terauchi et al.: "Isolation of a molt-inhibiting hormone from the sinus gland of the crayfish,Procambarus clarkii" Zool. Sci.(印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 長澤寛道(分担執筆): "昆虫の生化学・分子生物学" 名古屋大学出版会, 498 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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