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Structure of chemiluminescence substance contained in fermented foods and the scavenging mechanism of active oxygen radical

Research Project

Project/Area Number 07456057
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 食品科学・栄養科学
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

OKUBO Kazuyoshi  Tohoku University, Faculty of Agriculture, Professor, 農学部, 教授 (00005612)

Co-Investigator(Kenkyū-buntansha) IGARASHI Kiharu  Yamagata University, Faculty of Agriculture, Professor, 農学部, 教授 (00111336)
MURAMOTO Kouzi  Tohoku University, Faculty of Agriculture, Professor, 農学部, 教授 (90157800)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1995: ¥5,200,000 (Direct Cost: ¥5,200,000)
KeywordsFermented foods / Soybean / Leguminous plant / Radical scavenger / Chemiluminescence / XYZ system / Active oxygen species / DDMPサポニン
Research Abstract

The chemiluminescence (CL) of catechins were measured in the presence of active oxygen species and acetaldehyde (MeCHO). (-) -Epigallocatechin (EGC) exhibited the strongest CL among flavonoids, anthocyanins and catechins. The CL intensity, P,was dependent on the concentrations of active oxygen species, X,such as H_2O_2, catalytic species, Y,such as EGC and receptive species, Z,such as MeCHO as follows ; p=k [X] [Y] [Z] (k ; photon constant). From the results by HPLC and antioxidative effect against lipid autoxidation, the XYZ system is suggested to be a scavenging mechanism of active oxygen species. New substances isolated from soybean fermented foods showed storong charavteristics as Y and Z,which have storong antioxidative sctivity.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (46 results)

All Other

All Publications (46 results)

  • [Publications] M.Shimoyamada et al.: "Partition of siybean saponins between butanol and water" Food Sci.Technol.lnt.1. 18-21 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y, Yoshiki et al.: "Chemiluminescence of soybean saponins in the presence of active oxygen specier" Plant Science. 116. 125-129 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Yoshikoshi et al.: "Prevention of hydrogen peroxide damage by soybean saponins to mouse fibroblast cells," Planta Medica. 62. 252-255 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] R.Amarowicz et al.: "Hemolytic activity of the fractions separated from the rapeseed ethanolic extract" Nahrung. 40. S.155-157 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Yoshiki et al.: "Chemiluminescence mechanism of catechins in the presence of active oxygen" J.Biolumin.Chemilumin.11. 131-136 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Yoshikoshi et al.: "Soybean βg and gallic acid prevent damage of hydrogen peroxide to mouse fibroblast cells" Planta,Medica. 62. 193-288 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shimoyamada, M., et al: "Partition of siybean saponins between butanol and water" Food Sci.Technol.Int.1 (2). 18-21 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshiki, Y., et al.: "Chemiluminescence of soybean saponins in the presence of active oxygen species" Plant Science. 116. 125-129 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshikoshi, M., et al: "Prevention of hydrogen peroxide damage by soybean saponins to mouse fibroblast cells" Planta Medica. 62 (3). 252-255 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Amarowicz, R., et al.: "Hemolytic activity of the fractions separated from the rapeseed ethanolic extract" Nahrung. 40, Nr.3. 155-157 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshiki, Y., et al.: "Chemiluminescence mechanism of catechins in the presence of active oxygen" J.Biolumin.Chemilumin.11. 131-136 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshikoshi, M., et al: "Soybean betag and gallic acid prevent damage of hydrogen peroxide to mouse fibroblast cells" Planta medica. 62 (3). 193-288 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okubo, K: "Health-enhancing ingredients of soybean in food : oxygen radical scavenging characteristics of DDMP saponins and the related substances" Agri-Food Quality, An Interdisciplinary approach, eduted by G.R.Fenwick et al., The Royal Society of Chemistry. 351-359 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshiki, Y., et al.: "Hydrophilic oxygen radical scavengers in leguminous seeds and derived foods" Agri-Food Quality, An Interdisciplinary approach, eduted by G.R.Fenwick et al., The Royal Society of Chemistry. 360-363 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshiki, Y., et al.: "Chemiluminescence of phenolic compounds in tne presence of active oxygen species and acetaldehyde" Agri-Food Quality, An Interdisciplinary approach, eduted by G.R.Fenwick et al., The Royal Society of Chemistry. 364-3637 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okubo, K.and Yoshiki, Y.: "Oxygen-radical-scavenging activity of DDMP-conjugated saponins and physiological role in leguminous plant" Saponins Used in Food and Agriculture, edited by George R.Waller and Kazuo Yamasaki, Pleum Press, New York. (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshiki, Y., et al.: "Chemiluminescence of oxygen radical scavengers such as DDMP saponins in the presence of radicals and aldehyde" ibid.231-239 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshiki, Y., et al.: "Photon emisson by natural radical scavengers in the presence of active oxygen species" Proceedings of the International Conference on Foods Factors, edited by H.Ohigashi et al., Springer-Verlag Tokyo. 313-317 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] R.Amarowicz, R.B.et al.: "Fourier transform infrared (FTIR) spectra of soybean saponin Ab and Bb" Nahrung. 40. 342-343 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M. Shimoyamada et al.: "Partition of siybean saponins between butanol and water" Food Sci. Technol. Int.1. 18-21 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y, Yoshiki et al.: "Chemiluminescence of soybean saponins in the presence of active oxtgen species" Plant Science. 116. 125-129 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M. Yoshikoshi et al.: "Prevention of hydrogen peroxide damage by soybean saponins to mouse fibroblast cells," Planta Medica. 62. 252-255 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] R. Amarowixz et al.: "Hemolytic activity of the fractions separated from the rapeseed ethanolic extract" Nahrung. 40. S. 155-157 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y. Yoshiki et al.: "Chemiluminesence mechanism of catechins in the presence of active oxygen" J. Biolumin. Chemilumin.11. 131-136 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M. Yosjikoshi et al.: "Soybean βg and gallic acid prevent damage of hydrogen peroxide to mouse fibroblast cells" Planta Medica. 62. 193-288 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Yoshiki et al.: "Saponin conjugated with 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one from Dolichos lablab and the SOD-like activity." Phytochemistry. 38. 229-231 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Kanazawa et al.: "Protective effects of soy protein on the peroxidizability of lipoproteins in cerebrovascular deseases" J.Nutrition. 125. 639S-646S (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Yoshiki et al.: "Chemiluminescence of benzoic acid cinnamic acid,and flavonoids in the presence of aldehyde and hydroben peroxide or hydroxyl radical by Fenton reaction" Phytochemistry. 39. 225-229 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] C.Tsukamoto et al.: "Factors affecting isoflavone content in soybean seeds:Changes in contents of isoflavones,saponins and composition of fatty acids at different temperatures during seed development," J.Agric.Food Chem.,. 43. 1184-1192 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Yoshiki and K.Okubo: "Oxygen radical scavenging activity of DDMP(2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one)conjugated saponin in soybean seed." Biosci.Biotech.Biochem.,. 59. 1556-1557 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Yoshiki et al.: "Chemiluminescence of anthocyanins in the presence of aldehyde and tert-butyl hydroperoxide" J.Biolumin.Chemilumin.10. 335-338 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] J.H.Kim et al.: "Genetic studies on saponin content by Vr-Wr graphical analysis in soybean(Glycinr max)" Korea Soybean Digest. 12. 74-81 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] J.H.Kim et al.: "Analysis of GCA and SCA for saponin content in soybean hybrid population" Korea Soybean Digest. 12. 82-88 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Ohtsuki et al.: "A 96-kDa glycyrrhizin-binding protein(gp96)from soybeans scts as a substrate for casein kinase lland it is highly related to lipoxygenase 3." J.Biochem.,. 118. 1145-1150 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Yoshikoshi et al.: "Metabolism and nonabsorption of soybean hypocotyl saponins in the rat model." Acta Alimetaria. 24. 355-364 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Shimoyamada et al.: "Partition of siybean saponins between butanol and water" Food Sci.Technol.lnt.1. 18-21 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] R.Amarowicz et al.: "Hemolytic activity of the fractions from the rapeseed ethanolic extract," Fat Sci.Technol.(印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Yoshikoshi et al.: "Prevention of hydrogen peroxide damage by soybean saponins to mouse fibroblast cells," Planta med.62(印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Yoshiki et al.: "Chemiluminescence mechanism of catechins in the presence of active oxygen" J.Biolumin.Chemilumin.(印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Yoshiki et al.: "Chemiluminescence of soybean saponins in the presence of active oxygen species" Plant Science. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 大久保一良: "種子のバイオサイエンス" 学会出版センター, 462 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 大久保一良: "植物の世界45号、ダイズ、デイコ、アズキ分担" 朝日新聞社, 2 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 大久保一良: "機能性食品の設計-活性酸素消去機能分担" 学会出版センター, 6 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 吉城由美子・大久保一良: "がん予防食品の設計、天然ラジカルスカベンジャー分担" シ-・エム・シ-社, 10 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 大久保一良・吉城由美子: "医食同源-ダイズサポニンの抗酸化機能分担" 共立出版(印刷中), (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] S.Kudou,S.and K.Okubo: "CRC Handbook of Plant and Fungal Toxiccants:lsoflavones" CRC(印刷中), (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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