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Selectivity for Incorporation of n-3 and n-6 Unsaturated Fatty Acids into Phospholipids

Research Project

Project/Area Number 07456062
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 食品科学・栄養科学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

KITO Makoto  Kyoto University, Research Institute for Food Science, Professor, 食糧科学研究所, 教授 (60027183)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥5,000,000 (Direct Cost: ¥5,000,000)
Keywordsn-3 Unsaturated fatty acids / n-6 Unsaturated fatty acids / Phospholipids
Research Abstract

The selective incorporation of n-3 and n-6 unsaturated fatty acids into cell phospholipids was investigated by changing the ratio of n-3 unsaturated fatty acids against n-6 unsaturated fatty acids in the medium where Chinese hamster V79-R cells were grown. Unsaturated fatty acids with lower degrees of unsaturation were abundantly incorporated into phosphatidylcholine independently of n-3 and n-6. Unsaturated fatty acids with highter degrees of unsaturation were more predominantly incorporated into phosphatidylethanolamine. When the difference in the number of double bond between unsaturated fatty acids was more than two. the unsaturated fatty acids with a higher degree of unsaturation was more selectively incorporated into phosphatidylethanolamine than the unsaturated fatty acids with a lower dgree of unsaturation. By the analysis of molecular species. n-3 and n-6 unsaturated fatty acids with higher degrees of unsaturation were incorporated competitively into phosphatidylethanolamine. Finally, docosahexaenoic acid was incorporated into diacyl phosphatidylethanolamine more selectively than the other unsaturaed fatty acids.
In addition. the similar selectivity was found in phosphatidylcholine and phosphatidylethanolamine of mitochondria, endoplasmic reticulum and plasma membrane, independent of the difference among organelles.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] H. Masui, R. Urade, and M. Kito: "Selective Incorporation of n-3 and n-6 Unsaturated Fatty Acids into Aminal Cell Phospholipids" Biosci. Biotech. Biochem.59. 644-647 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H. Masui, R. Urade. and M. Kito: "Selective Incorporation of Polynusaturated Fatty Acids into Organelle Phospholipids of Aminal Cells" Biosci. Biotech. Biochem.61 (印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Masui, R.Urade, and M.Kito: "Selective Incorporation of n-3 and n-6 Unsaturated Fatty Acids into Aminal Cell Phospholipids" Biosci.Biotech.Biochem.59(4). 644-647 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Masui, R.Urade, and M.Kito: "Selective Incorporation of Polynusaturated Fatty Acids into Organelle Phospholipids of Aminal Cells" Biosci.Biotech.Biochem.61 (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Masui,R.Urade.& M.Kito: "Selective Incorporation of Polyunsaturated Fatty Acids into Organelle Phospholipids of Animal Cell" Biosci.Biotech.Biochem.(印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hironori Masui: "Selective Incorporation of n-3 and n-6 Unsaturated Fatty Acids into Aminal Cell Phospholipids" Biosci. Biotech. Biochem.59. 644-647 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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