| Co-Investigator(Kenkyū-buntansha) |
HIRONO Ikuo Tokyo University of Fisheries, Faculty of Fisheries, Assistant Professor, 水産学部, 助手 (00270926)
TAKASHIMA Fumio Tokyo University of Fisheries, Faculty of Fisheries, Professor, 水産学部, 教授 (60041703)
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| Budget Amount *help |
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1996: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1995: ¥4,400,000 (Direct Cost: ¥4,400,000)
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| Research Abstract |
1.We have cloned and characterized a medaka transferrin cDNA.Medaka transferrin gene was mainly transcribed in liver.
2.The medaka transferrin gene is approximately 8.5 kb, and it is organized into 17 exones separated by 16 introns. The 5'-flanking region of the medaka transferrin gene contains an glucocorticoid-responsive elements, metal responsive element, and etc.
3.Three genera including 9 species of salmonid fish transferrin cDNA were cloned and characterized. There were genus some genus specific amino acid residues.
4.We have investigated that there were 3 types of transferrin phenotype and some sub-type of transferrin in coho salmon and rainbow trout by starch gel electrophoresis analysis characterization of cDNA,respectively. However, the results from starch gel analysis and characterization of cDNA were not same.
5.Restriction fragment length polymorphism of the LA-PCR products transferrin genes from coho salmon and rainbow trout revealed several digestion patterns. These differences of digestion patterns resulted of differences of nucleotide sequences of introns of transferrin genes.
6.We have cloned several immune related molecules, such as MHC class II,class B,complement C2, C3, C9, Complement regulatory plasma protein, vitoronectin, 1g light chain, natural killer enhancement factor, M * 1, transferrin, TNF inducer protein, and transferrin from medaka liver, flounder liver and leukocytes, and eel spleen cDNA library by expressed sequence tag analysis.
7.An MHC class 1 cDNA clone was isolated and characterized from pink salmon. Polymorphism of the MHC class 1 alpha 1 domains was determined using PCR.
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