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Culture of the immunocompetent cells of aquatic animals and cell growth factors

Research Project

Project/Area Number 07456092
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionNIHON UNIVERSITY

Principal Investigator

WATANABE Tasuku  Nihon university, School of Bioresourse Science, Associate Professor, 生物資源科学部, 助教授 (30095517)

Co-Investigator(Kenkyū-buntansha) HIROSE Euichi  Nihon university, School of Bioresourse Science, Lecturer, 生物資源科学部, 講師 (30241772)
TOMANA Makoto  Nihon university, School of Bioresourse Science, Assistant Professor, 生物資源科学部, 助手 (10246853)
MORITOMO Tadaaki  Nihon university, School of Bioresourse Science, Lecturer, 生物資源科学部, 講師 (20239677)
ASAHINA Kiyoshi  Nihon university, School of Bioresourse Science, Associate Professor, 生物資源科学部, 助教授 (10147671)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1996: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1995: ¥3,900,000 (Direct Cost: ¥3,900,000)
Keywordscarp / red sea bream / colonial ascidian / B lymphocyte / macrophage / IgM / granulocyte / chemotactic factor
Research Abstract

The carp hematopoietic cells of pronephros can proliferate and form colonies in the presence of homologous carp serum. After 3-5 days of culture, colonies consisting of small round cells were observed. The number of cells in the colonies increased steadily. Colonies were formed on an adherent cell layr. The proliferated cells in the colonies were separated from the colony into the culture medium. These floating cells were constantly produced for more than one month. Morphologically, the majority of the floating cells had features of immature type of cells which were mainly round cells with basophilic cytoplasm and a round nucleus. Hidrocortisone enhanced the growth of granulocytes, whereas B lymphocytes proliferated predominantly in the absence of this hormone. Such a method contributes to the investigation of the roles of fish polymorpho-nuclear phagocytes and lymphocytes in host defense. Fish phagocytes is consisted of monocytes/macrophages and granulocytes. We tried to culture the r … More ed sea bream and obtained transferable cultured cells. The cells had phagocytotic activity against Latex beads and had acid phosphatase and nonspecific esterase activities. Electron microscopically, the cells had slender microvilli, micropinocytotic vesicle-like vacuoles, and microfibrils, similar to red sea bream resident peritoncal macrophages. The cells had receptors for red sea bream IgM on cell surface. The cultured cells migrated to FMLP and the supernatants of the cultured cells. The results suggest that resident peritoneal macrophages produce and release chemotactic factor-line substances in abdominal cavity. In a colonial ascidian, physical stimulations induce strong luminescence in the tunic. Three types of tunice cells are morphologically discriminated as morula-like tunic cells, tunic phagocytes, and tunic granulocytes. Tunic phagocytes contain round granules, clear vacuoles, and occasionally phagosomes. Tunic granulocytes characterized by a number of elliptical granules and they occasionally contain phagosomes and round granules that are similar in structure to tunic phagocytes. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Euichi Hirose: "Regenevation of the tunic cuticle in the compound ascidian, Botrylloides simodensis." Developmental and Comparative Immunology. 19. 143-151 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Euichi Hirose: "Microfilament contraction promotes rounding of tnnic slices : An integumentory detensesystem in the colonial ascidian." Biological Bulletin. 189. 29-35 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tasuku Watanabe: "Resident peritoneal cells of red sea bream Pagrusmajor" Fisheries Science. 61. 937-941 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tadaaki Moritomo: "Culture of carp hematopoietic cells" Fish Pathology. 31. 59-63 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Euichi Hirose: "Fine structure of tunic cells and distribution of bacteria in the tunic of the luminescent ascidian clavelina miniata" Zoological Science. 13. 519-523 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] E.Hirose: "Reguration of the tunic cuticle in the compound ascidian, Botrylloides simodensis" Developmental and Comparative Immunology. 31. 143-151 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] E.Hirose: "Microfilament contraction promotes rounding of tunic slices : An integumentory defense system in the colonial ascidian" Biological Bulletin. 189. 29-35 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Watanabe: "Resident peritoneal cells of red sea bream Pagrus major" Fisheries Science. 61. 937-941 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Moritomo: "Culture of carp hematopoietic cells" Fish Pathology. 31. 59-63 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] E.Hirose: "Fine structure of tunic cells and distribution of bacteria in the tunic of the luminescent ascidian Clavelina miniata" Zoological Science. 13. 519-523 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Euichi Hirose: "Regeneration of the tunic cuticle in the compound Ascidian,Botrylloides simodensis." Developmental and Comparative Immunology. 19. 143-151 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Euichi Hirose: "Microtilament contraction promotes rounding of tunic slices : An integumentary detense system in the colonial Ascidian" Biological Bulletin. 189. 29-35 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tasuku Watanabe: "Resident Peritoneal cells of red sea bream Pagrus major" Fisheries Science. 61. 937-941 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tadaaki Moritomo: "Culture of carp hematopoietic cells" Fish Pathology. 31. 59-63 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Euichi Hirose: "Fine structures of tunic cells and distridution of dacteria in the tunic of the luminescent ascidian clavelind miniata" Zoological Science. 13. 519-523 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Euichi Hirose: "Regeneration of the tunic cuticle in the compound Ascidiam, Borrylloides simodensis" Developnental and Comparative Immunology. 19. 143-151 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Euichi Hirose: "Microfilament Comtraction Pronotes Rounding of Tunic Slices: An Integunentary Defense System in the Colonial Ascidian" Biological Bulletin. 189. 29-35 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tasuku Watanabe: "Resident Peritoneal Cells of Red Sea Bream Pagrus major" Fisheries Science. 61. 937-941 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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