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Mammalian Reproductive Pheromone : Isolation and Study on Central Action

Research Project

Project/Area Number 07456122
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionThe University of Tokyo

Principal Investigator

MORI Yuji  Univ.of Tokyo, Grad.Sch.of Agri.and Life Sci., Associate Profeffor, 大学院・農学生命科学研究科, 助教授 (40157871)

Co-Investigator(Kenkyū-buntansha) TAKEUCHI Yukari  Univ.of Tokyo, Grad.Sch.of Agri.and Life Sci., Assistant Profeffor, 大学院・農学生命科学研究科, 助手 (10240730)
YAMAGUCHI Isomaro  Univ.of Tokyo, Grad.Sch.of Agri.and Life Sci., Associate Profeffor, 大学院・農学生命科学研究科, 助教授 (00012013)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥8,700,000 (Direct Cost: ¥8,700,000)
Fiscal Year 1996: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1995: ¥6,800,000 (Direct Cost: ¥6,800,000)
KeywordsPheromone / GnRH / Multipleunit activity / Neurotransmitters / Reproductive endocrinology / Hypothalamus / Olfactory system / Shiba goat / CuRH / 神経伝達物資 / 生殖内分質
Research Abstract

In this study, the neurophysiological bioassay system for mammalian reproductive pheromones has been established by means of hypothalamic multipleunit activiry (MUA) recording, by which the GnRH pulse generator activity can be monitored conveniently. The porposes of the present study were [1] to purify the pheromonal substance responsible for the "male effect" known in domestic ruminant species such as sheep and goats, and [2] reveal the central mechanism of pheromone actions which not only stimulate reproductive activities but also modify various other behaviors including feeding and exploring. Obtained results can be summarized as follows.
Recording electrodes were stereotaxically placed in the medial basal hypothalamus of ovariectomized goats, which were subcutaneously implanted with estradiol containing capsules to keep steroidal milieu constant. The specific MUA volleys, which coincided with LH pulses in the peripheral circulation, were regarded as the electrophysiological manifest … More ation of the GnRH pulse generator activity. The exposure of the tested-goat to hair sample, or its ether extract, obtained from sexually-active male goats resulted in the accleleration of the GnRH pulse generator activity, clearly indicating the existence of primer-pheromone activity. On the other hand, hair samples from castrated male did not have such a progonadal activity, which was then resumed after treating the castrated one with testosterone for several weeks. Sebaceous gland was suggested to be the potential source of androgen dependently produced male pheromones.
In addition to single (short-term) appliation, more prolonged exposure (e.g.4 hours continuously) to pheromes was also effective in terms of stimulating the reproductive neuroendocrine system. Another interesting finding was that the goat developed a state of reflactory to the pheromonal stimulation following continuous exposure for several hours. Based on the neurophysiological bioassay means developed in this study, the isolation study of pheromone (s) is proceeding. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Yoshida J.,Osada T.,Mori Y.,Ichikawa M.: "Differential binding patterns of three antibodies (VOBM1,VOBM2 and VOM2)in the rat vomeronasal organ and accessory olfactory bulb." Cell Tissue Res.281. 243-248 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mori Y.,Tanaka T.: "Electrophysiological approach to the hypothalamic GnRH pulse generator." J.Reprod.Fertil.Suppl.49. 231-243 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ichikawa M.,Matsuoka M.,Mori Y.: "Plastic effect of soiled bedding on the structure of synapses in rat accessory olfactry bulb." Synapse. 21. 104-109 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 森裕司,濱田知宏: "鋤鼻嗅覚神経系の行動学的解析" 蛋白質核酸酵素. 551. 2084-2092 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hamada T.,Nakajima M.,Takeuchi Y.,Mori Y.: "Pheromone induced stimulation of hypothalamic gonadotropin-releasing formone pulse generator activity in ovariectomized,estrogen-primed goats." Neuroendocrinology. 64. 313-319 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuoka M.,Mori Y.,Ichikawa M.: "Morphology of environmentally induced synaptic plasticity in vomeronasal system" Neuroreport. 7. 2347-2352 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mori Y.,Tanaka T.,Hamada T.,Takeuchi Y.: "Neural Control of Reproduction Physiology and Behavior" 学会出版センター(in press), (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshida J., Osada T., Mori Y., Ichikawa M.: "Differential binding patterns of three antibodies (VOBM1, VOBM2 and VOM2) in the rat vomeronasal organ and accessory olfactory bulb" Cell Tissue Res.281. 243-248 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mori Y., Tanaka T.: "Electrophysiological approach to the hypothalamic GnRH pulse generator" J.Reprod.Fert.Suppl.49. 231-243 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ichikawa M., Matsuoka M., Mori Y.: "Plastic effect of soiled bedding on the structure of synapses in rat accessory olfactry bulb" Synapse. 21. 104-109 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hamada T., Nakajima M., Takeuchi Y., Mori Y.: "Pheromone-induced stimulation of hypothalamic gonadotropin-releasing hormone pulse generator activity in ovariectomized, estrogen-primed goats" Neuroendocrinology. 64. 313-319 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mori Y., Tanaka T., Hamada T., Takeuchi Y.: "Electrophysiological correlated of GnRH secretion" Anim.Reprod.Sci.42. 537-550 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuoka M., Mori Y., Ichikawa M.: "Morphology of environmentally induced synaptic plasticity in vomeronasal system" Neuroreport. 7. 2347-2352 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mori Y., Tanaka T., Hamada T., Takeuchi Y.: "External and internal signals regulating GnRH secretion in ruminant species" Neural control of reproduction. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hamada T.,Nakajima M.,Takeuchi Y.,Mori Y.: "Pheromone induced stimulation of hypothalamic gonadotropin releasing hormone pulse generator activity in ovariectomized,estrogen primed goats." Neuroendocrinology. 64. 313-319 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mori Y.,Tanaka T.,Hamada T.,Takeuchi Y.: "Electrophysiological correlates of GnRH secretion." Anim.Reprod.Sci.42. 537-550 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 森裕司,濱田知宏: "鋤鼻嗅覚神経系の行動学的解析." 蛋白質核酸酵素. 551. 2084-2092 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Okada M.,Hamada T.,Takeuchi Y.,Mori Y.: "Timing of proceptive and receptive behavior of female goats in relation to the preovulatory LH surge." J.Vet.Med.Sci.58. 1085-1089 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsuoka M.,Mori Y.,Ichikawa M.: "Morphology of environmentally induced synaptic plasticity in vomeronasal system." Neuroreport. 7. 2347-2352 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takeuchi Y.,Nagabukuro H.,Kizumi O.,Mori Y.: "Lipopolysaccharide induced suppression of the hypothalamic gonadotropin releasing hormone pulse generator in ovariectomized goats." J.Vet.Med.Sci.59. 93-96 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mori Y.,Tanaka T.,Hamada T.,Takeuchi Y.: "Neural Control of Reproduction:Physiology and Behavior" 学会出版センター(in press), (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ichikawa M,Matsuoka M,Mori Y: "Plastic effect of soiled bedding on the structure of synapses in rat accessory olfactry bulb." Synapse. 21. 104-109 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Mori Y,Tanaka T: "Electrophysiological approach to the hypothalamic GnRH pulse generator" J.Reprod. Fert Suppl.49. 231-243 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoshida J,Osada T,Mori Y,Ichikawa M: "Differential binding pattrrns of three antibodies (VOBM1,VOBN2 and VOM2) in the rat vomeronasal organ and accessory olfactory bulb." Cell Tissue Res. 281. 243-248 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kanai Y,Yamasaki J,Takeuchi Y,Koike N,Fujiyama M,Mori Y: "Electrophysiological recording of hypothalamic GnRH pluse genetator activity in castrated male goat." J. Reprod. Develop. 41. 57-62 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tanaka T,Ozawa T,Hoshino K,Mori Y20GB05:Changes in the gonadotropin-releasing hormone pulse generator activity during the estrous cycle in the goat.: Neuroendocrindogy. 62. 553-561 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Zuccolilli GO,Mori Y: "Cranial and cerebral-ventricular landmarks for accurate Stereotaxic approach to hypothalamic nuclei of the goat brain." J.Vet.Med SCi. 57. 453-457 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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