| Project/Area Number |
07456134
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
MATSUDA Hiroshi Tokyo University of Agriculture and Technology, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (80145820)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Gen Tokyo University of Agriculture and Technology, Faculty of Agriculture, Associat, 農学部, 助教授 (90158626)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | inflammatory cytokine / nerve growth factor / mast cells / nerve growth factor receptors / cytokine network / inflammatory mediators / mouse / rat / 神経生長因子 |
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| Research Abstract |
Mast cells are the primary initiating cells of immediate hypersensitivity reactions and also function as an effector cell in the process of inflammatory reaction and tissue remodeling : In the present research project, we investigated activation of mast cells by nerve growth factor (NGF) which has broader biologic activities on immunocompetent cells. The obtained results are as follows :
1) Murine mast cell lines expressed trkA receptors, unlike p75 receptors, for NGF.
2) Mast cells differentiate into two phenotypically distinct populations : connective tissue-type mast cells and mucosal mast cells. Bone marrow derived-cultured mast cells, appear to be their precursors, had both of trkA and p75 receptors and peritoneal mast cells (connective tissue-type mast cells) expressed trkA receptors, but not p75 receptors.
3) NGF suppressed apoptosis of rat peritoneal mast cells through the trkA receptor and inhibited transforming growth factor beta1-induced apoptosis.
4) NGF induced serotonin release from rat peritoneal mast cells in the presence of activated platelets.
5) NGF was able to activate mast cells through phosphatidylserine derived from platelets. These results suggest that NGF may have support survival and induce activation of mast cells. Thus, NGF may play as a regulatory cytokine in the reactions involving in mast cells.
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